Camellia sinensis

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Description

Camellia sinensis.png
  • Camellia sinensis is the most consumed beverage in the world aside from water, and has many beneficial health effects. It is an important cash crop, and is grown commercially in about 30 different countries. It is manufactured by drying fresh tea leaves and contains characteristic polyphenolic compounds, (–)-epigallocatechin-3-gallate (EGCG), (–)-epigallocatechin (EGC), (–)-epicatechin-3-gallate (ECG) and (–)-epicatechin (EC). These compounds are commonly known as catechins[1][2].
  • Common Name: Green tea
  • NCBI Taxonomy

Various Experimental Treatments

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
CsPTB1[1] Polypyrimidine tract-binding protein
  • Sampled at different time of a day
  • Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots
  • Cold treatment
GAAC01052498.1
  • F:TGACCAAGCACACTCCACACTATCG
  • R:TGCCCCCTTATCATCATCCACAA
107 60 SYBR
CsEF1[1] Elongation factor 1 alpha
  • Sampled at different time of a day
  • Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots
  • Cold treatment
KA280301.1
  • F:TTGGACAAGCTCAAGGCTGAACG
  • R:ATGGCCAGGAGCATCAATGACAGT
110 60 SYBR
CsSAND1[1] SAND family protein
  • Sampled at different time of a day
  • Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots
  • Cold treatment
KM057790
  • F:TCCAATTGCCCCCTTAATGACTCA
  • R:GTAAGGGCAGGCAAACACCAGGTA
109 60 SYBR
CsCLATHRIN1[1] Clathrin adaptor complex subunit
  • Sampled at different time of a day
  • Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots
  • Cold treatment
KA291473.1
  • F:TAGAGCGGGTAGTGGAGACCTCGTT
  • R:TACCAAAGCCGGCTCGTATGAGATT
129 60 SYBR
CsUBC1[1] Ubiquitin-conjugating enzyme
  • Sampled at different time of a day
  • Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots
  • Cold treatment
KA281185.1
  • F:TGCTGGTGGGGTTTTTCTTGTTACC
  • R:AAGGCATATGCTCCCATTGCTGTTT
124 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Bin Xiao
  • Email: xiaobin2093@sohu.com
  • Institution: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China

Citation Statistics

Cited by 31 (Based on Google Scholar [2017-09-01])

Leaf development & Hormonal Treatment

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
CsTBP[3] TATA-box binding protein gene
  • Leaf development and metabolism
  • Hormonal stimuli
AT5G09810 (Arabidopsis homolog gene)
  • F:GGCGGATCAAGTGTTGGAAGGGAG
  • R:ACGCTTGGGATTGTATTCGGCATTA
166 85 SYBR
CsTIP41[3] Tap42-interacting protein of 41 kDa gene
  • Leaf development and metabolism
AT4G34270 (Arabidopsis homolog gene)
  • F:TGGAGTTGGAAGTGGACGAGACCGA
  • R:CTCTGGAAAGTGGGATGTTTGAAGC
176 87 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jing Zhuang
  • Email: zhuangjing@njau.edu.cn
  • Institution: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

Citation Statistics

Cited by 18 (Based on Google Scholar [2017-09-01])

Biotic Stress & Hormonal Treatment

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
miR159a[4] miR159a
  • Bud to the fifth leaf
  • Methyl jasmonate (MeJA) Treatment
  • Salicylic acid (SA) Treatment
  • Abscisic acid (ABA)
NA
  • F:CGCGCTTGGATTGAAGGG
  • R:NA
NA 60 SYBR
5S rRNA[4] 5S rRNA
  • Different organs
NA
  • F:GCGATCATACCAGCACCAATG
  • R:CAACACAAGGACTTCCTAGGG
NA 60 SYBR
miR6149[4] miR6149
  • Leaves were attacked by Ectropis oblique and U4
NA
  • F:GCGCGATACGCACCTGAAT
  • R:NA
NA 60 SYBR
miR5368n[4] miR5368n
  • Methyl jasmonate (MeJA) Treatment
  • Salicylic acid (SA) Treatment
  • Abscisic acid (ABA) Treatment
NA
  • F:GCGGAGATACCACTCTGG
  • R:NA
NA 60 SYBR

Molecular Types

  • miRNA

Evaluation Methods

Contact

  • Name: Chaoling Wei
  • Email: weichl@ahau.edu.cn;
  • Institution: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China

Citation Statistics

Cited by 3 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Hao X, Horvath D P, Chao W S, et al. Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze)[J]. International journal of molecular sciences, 2014, 15(12): 22155-22172.
  2. Yang C S, Landau J M. Effects of tea consumption on nutrition and health[J]. The Journal of nutrition, 2000, 130(10): 2409-2412.
  3. 3.0 3.1 Wu Z J, Tian C, Jiang Q, et al. Selection of suitable reference genes for qRT-PCR normalization during leaf development and hormonal stimuli in tea plant (Camellia sinensis)[J]. Scientific reports, 2016, 6.
  4. 4.0 4.1 4.2 4.3 Song H, Zhang X, Shi C, et al. Selection and verification of candidate reference genes for mature microRNA expression by quantitative RT-PCR in the tea plant (Camellia sinensis)[J]. Genes, 2016, 7(6): 25.

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