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  • Chloromonas (Cr.), is used as a model organism for molecular biology, is a genus of green algae consisting of unicellular flagellates, found in stagnant water, damp soil, freshwater, seawater, and even in snow. It is a unicellular biflagellate in the green algal order Volvocales, is traditionally distinguished from its close relative, Chlamydomonas (Cd.), by lacking pyrenoids in its chloroplasts. Chloromonas has more species identified from cold habitats, and many of these taxa reside in snow[1][2].
  • NCBI Taxonomy

Freezing Acclimation

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
Size [bp] Tm [℃] Detection
RPL19[1] 60S ribosomal protein L19
  • Freezing stress
150 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods


  • Name: Chenlin Liu
  • Email:
  • Institution: The First Institute of Oceanography, Marine Ecological Center, State Oceanic Administration, Qingdao 266061, China

Citation Statistics

Cited by 23 (Based on Google Scholar [2017-09-01])


  1. 1.0 1.1 Liu C, Wu G, Huang X, et al. Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation[J]. Extremophiles, 2012, 16(3): 419-425.
  2. Barsalobres-Cavallari C, Severino F, Maluf M, Maia I. Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions. BMC Mol Biol 10(1):1.