Downloads

ICG Sequence Download

To promote the study on identification and characterization of potential internal control gene candidates from newly sequenced genomes or transcriptomes, sequence set of the experimental verified internal control genes ( in FASTA Format) are provided for users to freely Download.

Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources (e.g., NCBI accession number) were obtained from the related peer-reviewed literature.
You can get access to the sequences directly with linux command (for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa).
The potential internal control gene candidates from the users' own data set are proposed to be identified through BLASTN search, against the curated sequences.
By the way, for primer design of potential identified internal control genes, an example of parameters for PRIMER 3.0 is as followings :

(a) Product size: 100-250 bp;

(b) Primer size: 19-25 bp;

(c) GC content : 45-55 %;

(d) Primer Tm : 56-62°C;