Mus musculus

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Description

Mus musculus.png
  • Mus musculus is a small mammal of the order Rodentia, characteristically having a pointed snout, small rounded ears, and a long naked or almost hairless tail. It is one of the most numerous species of the genus Mus. Although a wild animal, the house mouse mainly lives in association with humans (Modified from Wikipedia).
  • Common Name: Mouse
  • NCBI Taxonomy

Dextran Sodium Sulfate Experimental Colitis

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Tbp[1] TATA-box-binding protein
  • Mouse dextran sodium sulfate experimental colitis
NM_013684
  • F:ACCGTGAATCTTGGCTGTAAAC
  • R:GCAGCAAATCGCTTGGGATTA
86 60.8~61.3 SYBR
Eef2[1] Eukaryotic Translation Elongation Factor 2
  • Mouse dextran sodium sulfate experimental colitis
NM_007907.2
  • F:TGTCAGTCATCGCCCATGTG
  • R:CATCCTTGCGAGTGTCAGTGA
123 62.1~62.2 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jean-Eric Ghia
  • Email: Jean-Eric.Ghia@umanitoba.ca
  • Institution: Immunology, University of Manitoba, Winnipeg, MB, Canada

Citation Statistics

Cited by 3 (Based on Google Scholar [2017-09-01])

Mammary Glands

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
HPRT[2] Hypoxanthine guanine phosphoribosyl transferase
  • Mouse mammary glands
NM_013556
  • F:CCTAAGATGAGCGCAAGTTGAA
  • R:CCACAGGACTAGAACACCTGCTAA
86 60 SYBR
RPL13A[2] Ribosomal protein L13A
  • Mouse mammary glands
NM_009438
  • F:CCTAAGATGAGCGCAAGTTGAA
  • R:CCACAGGACTAGAACACCTGCTAA
168 60 SYBR
GAPDH[2] Glyceraldehyde-3-phosphate dehydrogenase
  • Mouse mammary glands
NM_008084
  • F:TGTGTCCGTCGTGGATCTGA
  • R:TTGCTGTTGAAGTCGCAGGAG
150 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Y.L. Wang
  • Email: ylwang2001@yahoo.cn
  • Institution: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China

Citation Statistics

Cited by 11 (Based on Google Scholar [2017-09-01])

Uterus in the Peri-Implantation Period

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
RPLP0[3] Ribosomal protein, large, P0
  • Early pregnancy, pseudopregnancy, delayed implantation
  • Activation, artificial decidualization
  • Hormonal treatment model
NM_007475
  • F:GGACCCGAGAAGACCTCCTT
  • R:GCACATCACTCAGAATTTCAATGG
85 60 SYBR
GAPDH[3] Glyceraldehydes-3-phosphate dehydrogenase
  • Early pregnancy, pseudopregnancy, delayed implantation
  • Activation, artificial decidualization
  • Hormonal treatment model
NM_008084
  • F:TCACTGCCACCCAGAAGA
  • R:GACGGACACATTGGGGGTAG
186 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: AiHua Wang
  • Email: pflin2001n@163.com
  • Institution: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China

Citation Statistics

Cited by 28 (Based on Google Scholar [2017-09-01])

Different Tissues & Ageing

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Rpl13a[4] Ribosomal protein L13a
  • Cerebral cortex during ageing
  • Cortex during ageing
  • Hippocampus during ageing
NM_009438.5
  • F:TACGCTGTGAAGGCATCAAC
  • R:GGGAGGGGTTGGTATTCATC
96 60 SYBR
GAPDH[4] Glyceraldehyde-3-phosphate dehydrogenase
  • Cerebral cortex during ageing
  • Cortex during ageing
  • Cerebellum during ageing
NM_008084.3
  • F:TGCGACTTCAACAGCAACTC
  • R:ATGTAGGCAATGAGGTCCAC
143 60 SYBR
Ppib[4] Peptidylprolyl isomerase (Cyclophilin) B
  • Striatum during ageing
  • Cerebellum during ageing
NM_011149.2
  • F:CAGCAAGTTCCATCGTGTCA
  • R:GATGCTCTTTCCTCCTGTGC
85 60 SYBR
Hprt[4] Hypoxanthine guanine phosphoribosyl transferase
  • Hippocampus during ageing
  • Cerebellum during ageing
NM_013556.2
  • F:CTTTGCTGACCTGCTGGATT
  • R:TATGTCCCCCGTTGACTGAT
121 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: B.D.Roussel
  • Email: broussel@cyceron.fr
  • Institution: INSERM, INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP CYCERON, University Caen Basse Normandie, boulevard Becquerel, 14074 Caen, France

Citation Statistics

Cited by 6 (Based on Google Scholar [2017-09-01])

Lung Development

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Tuba1a[5] Alpha 1a tubulin
  • Different stages of lung development
NM_011653
  • F:CCGCGAAGCAGCAACCAT
  • R:CCAGGTCTACGAACACTGCC
227 60.39~61.43 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Guillermo Barreto
  • Email: guillermo.barreto@mpi-bn.mpg.de
  • Institution: LOEWE Research Group Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research, Parkstraße 1, 61231 Bad Nauheim, Germany

Citation Statistics

Cited by 7 (Based on Google Scholar [2017-09-01])

Epithelial & Nonepithelial Cells

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
GUSB[6] Beta-glucuronidase
  • Eepithelial cells isolated from the mouse intestine
  • Eepithelial cells isolated the IEC6 cell line
NM_010368
  • F:CCGATTATCCAGAGCGAGTATG
  • R:CTCAGCGGTGACTGGTTCG
197 55–60 SYBR
TBP[6] TATA-box binding protein
  • Eepithelial cells isolated from the mouse intestine
  • Eepithelial cells isolated the IEC6 cell line
NM_008907
  • F:CAAACCCAGAATTGTTCTCCTT
  • R:ATGTGGTCTTCCTGAATCCCT
131 55–60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Yongping Su
  • Email: suyp2003@yahoo.com.cn
  • Institution: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China

Citation Statistics

Cited by 55 (Based on Google Scholar [2017-09-01])

Preimplantation Embryos

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
h2afz[7] H2A histone family, member Z
  • B6D2F-1 and C57BL/6 strains
  • Blastocyst embryonic stages cultured in KSOM medium
  • KOSOM and CZB cultures of B6D2F-1 and C57BL/6 strain-derived embryos
  • Zygote stage embryos
  • 2-cell, 4-cell, 8-cell stage embryos cultured in M16 medium
NM_016750
  • F:GTGGACTGTATCTCTGTGAA
  • R:GGTTGGTTGGAAGGCTAA
89 60 SYBR
sptbn[7] Pectrin beta, non-erythrocytic 1
  • ICR strain-derived embryos
NM_175836
  • F:TCTAATGGTTACTTGCTTGT
  • R:CAATAGTTACAGTGACAGAGA
101 55 SYBR
wrnip[7] Werner helicase interacting protein 1
  • M16 culture of B6D2F-1
  • C57BL/6 strain-derived embryos
  • 2-cell, 4-cell stage embryos cultured in CZB medium
  • Blastocyst stage embryos cultured in M16 medium
NM_030215
  • F:ATGAGTAGGATGCTTGTA
  • R:TAACCACCTCCATCTATG
130 56 SYBR
ywhaz[7] 14-3-3 protein zeta/delta (14-3-3ζ)
  • Zygote & molular embryonic stages cultured in KSOM medium
  • Molular stage embryos cultured in CZB medium
NM_001253807
  • F:CAGTAGATGGAGAAAGATTTGC
  • R:GGGACAATTAGGGAAGTAAGT
92 60 SYBR
tgfb1[7] Transforming growth factor, beta 1
  • 2-cell embryonic stages cultured in KSOM medium
NM_011577
  • F:TATACTGAGACACCTTGG
  • R:GTGATAGTCCTGAATAATTTG
83 55 SYBR
18s[7] 18S rRNA
  • 4-cell, 8-cell embryonic stages cultured in KSOM medium
  • Zygote embryos cultured in M16 medium
X00686
  • F:CGCCGCTAGAGGTGAAATTCT
  • R:CGAACCTCCGACTTTCGTTCT
102 60 SYBR
actb[7] β-actin
  • 8-cell stage embryos cultured in M16 medium
NM_007393
  • F:ATCTTCCGCCTTAATACT
  • R:GCCTTCATACATCAAGTT
98 56 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jin-Hoi Kim
  • Email: jhkim541@konkuk.ac.kr
  • Institution: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea

Citation Statistics

Cited by 4 (Based on Google Scholar [2017-09-01])

Embryonic Stem Cells

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Pgk1[8] Phosphoglycerate kinase 1
  • ES, TS and XEN stem cells
  • In vitro differentiation of embryonic and trophectoderm stem cells
NM_008828
  • F:CTGACTTTGGACAAGCTGGACG
  • R:GCAGCCTTGATCCTTTGGTTG
110 60 SYBR
Sdha[8] Succinate dehydrogenase complex, subunit A
  • ES, TS and XEN stem cells
  • In vitro differentiation of embryonic and trophectoderm stem cells
BC011301
  • F:GCTCCTGCCTCTGTGGTTGA
  • R:AGCAACACCGATGAGCCTG
134 60 SYBR
Tbp[8] TATA box binding protein
  • ES, TS and XEN stem cells
  • In vitro differentiation of embryonic and trophectoderm stem cells
NM_013684
  • F:GAAGAACAATCCAGACTAGCAGCA
  • R:CCTTATAGGGAACTTCACATCACAG
127 60 SYBR
Ywhaz[8] Tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • In vitro differentiation of embryonic and trophectoderm stem cells
NM_013684
  • F:TTGATCCCCAATGCTTCGC
  • R:CAGCAACCTCGGCCAAGTAA
88 60 SYBR
Hk2[8] Hexokinase 2
  • In vitro differentiation of embryonic and trophectoderm stem cells
NM_011740
  • F:CCCTGTGAAGATGTTGCCCAC
  • R:TGCCCATGTACTCAAGGAAGT
251 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Michael C. Golding
  • Email: mgolding@cvm.tamu.edu
  • Institution: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America

Citation Statistics

Cited by 42 (Based on Google Scholar [2017-09-01])

Obesity Model

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
B2M[9] Beta 2 Microglobulin
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
NM_009735
  • F:GGTCTTTCTGGTGCTTGTCTCA
  • R:GTTCGGCTTCCCATTCTCC
103 83.5 SYBR
18S[9] 18S Ribosomal RNA
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
NR_003278
  • F:GGCCGTTCTTAGTTGGTGGAGCG
  • R:CTGAACGCCACTTGTCCCTC
133 85 SYBR
HPRT1[9] Hypoxanthine phosphoribosyl transferase
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
NM_013556
  • F:CAGTCCCAGCGTCGTGATTA
  • R:GGCCTCCCATCTCCTTCATG
167 81.5 SYBR
RPlP0[9] Ribosomal Protein large P0
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
NM_007475
  • F:GATGGGCAACTGTACCTGACTG
  • R:CTGGGCTCCTCTTGGAATG
136 87.5 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Petra Matoušková
  • Email: matousp7@faf.cuni.cz
  • Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Citation Statistics

Cited by 26 (Based on Google Scholar [2017-09-01])

Testis Development

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Ppia[10] Peptidylprolyl isomerase (Cyclophilin) A
  • Testis development
NM_008907
  • F:TGTGCCAGGGTGGTGACTT
  • R:CGTTTGTGTTTGGTCCAGCAT
144 60 SYBR
Gapdh[10] Glyceraldehyde-3-phosphate dehydrogenase
  • Testis development
NM_008084
  • F:TGTGTCCGTCGTGGATCTGA
  • R:TTGCTGTTGAAGTCGCAGGAG
150 60 SYBR
Actb[10] β-Actin
  • Testis development
NM_007393
  • F:CATCCGTAAAGACCTCTATGCCAAC
  • R:ATGGAGCCACCGATCCACA
171 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Wen Zhen Lin
  • Email: linwenzhen@msn.com
  • Institution: Department of Biochemistry and Molecular Biology, School of Preclinical Sciences, Guangxi Medical University, Nanning 530021, Guangxi, China

Citation Statistics

Cited by 10 (Based on Google Scholar [2017-09-01])

Intestinal Epithelial Cells

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Ppib[11] Peptidylprolyl isomerase (Cyclophilin) B
  • Univeral reference gene in the differentiated fraction
NM_011149.2
  • F:CACCAATGGCTCACAGTTCTT
  • R:ATGACATCCTTCAGTGGCTTG
156 60 SYBR
Ppia[11] Peptidyl-prolyl cis–trans isomerase (Cyclophilin) A
  • Proliferating intestinal compartments and IEC6 cells
NM_008907.1
  • F:CCACTGTCGCTTTTCGCCGC
  • R:TGCAAACAGCTCGAAGGAGACGC
109 60 SYBR
Gapdh[11] Glyceraldehyde-3-phosphate dehydrogenase
  • Univeral reference gene in the differentiated fraction
XM_003945995.1
  • F:AGCAAGGACACTGAGCAAGAG
  • R:AGCAAGGACACTGAGCAAGAG
152 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Maria Sirakov
  • Email: maria.sirakov@ulb.ac.be
  • Institution: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies, Belgium

Citation Statistics

Cited by 5 (Based on Google Scholar [2017-09-01])

Skeletal Muscle & Different Genotypes

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Rpl27[12] Ribosomal protein L27
  • R129 strain
NM_011289
  • F:AAGCCGTCATCGTGAAGAACA
  • R:CTTGATCTTGGATCGCTTGGC
143 65 SYBR
Rer1[12] Retention in endoplasmic reticulum 1 protein
  • R129 strain
  • C57BL/6j strain
  • C57BL/10 strain
NM_026395
  • F:GCCTTGGGAATTTACCACCT
  • R:CTTCGAATGAAGGGACGAAA
137 62 SYBR
Rpl41[12] Ribosomal protein L41
  • R129 strain and Rpl7l1
  • C57BL/6j strain
NM_018860
  • F:GCCATGAGAGCGAAGTGG
  • R:CTCCTGCAGGCGTCGTAG
113 65 SYBR
Rpl7l1[12] Ribosomal protein L7-like 1
  • C57BL/6j strain
  • C57BL/10 strain
NM_025433
  • F:ACGGTGGAGCCTTATGTGAC
  • R:TCCGTCAGAGGGACTGTCTT
110 65 SYBR
Aldoa[12] Aldolase A, fructose-bisphosphate
  • C57BL/10 strain
NM_001177307
  • F:ACATTGCTGAAGCCCAACAT
  • R:ACAGGAAAGTGACCCCAGTG
136 62 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Kathryn N. North
  • Email: Kathryn.north@mcri.edu.au
  • Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Citation Statistics

Cited by 12 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 Eissa N, Hussein H, Wang H, et al. Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis[J]. PloS one, 2016, 11(5): e0156289.
  2. 2.0 2.1 2.2 Han L Q, Yang G Y, Zhu H S, et al. Selection and use of reference genes in mouse mammary glands[J]. Genet Mol Res, 2010, 9(1): 449-56.
  3. 3.0 3.1 Lin P F, Lan X L, Chen F L, et al. Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period[J]. PloS one, 2013, 8(4): e62462.
  4. 4.0 4.1 4.2 4.3 Bruckert G, Vivien D, Docagne F, et al. Normalization of Reverse Transcription Quantitative PCR Data During Ageing in Distinct Cerebral Structures[J]. Molecular neurobiology, 2016, 53(3): 1540-1550.
  5. Mehta A, Dobersch S, Dammann R H, et al. Validation of Tuba1a as appropriate internal control for normalization of gene expression analysis during mouse lung development[J]. International journal of molecular sciences, 2015, 16(3): 4492-4511.
  6. 6.0 6.1 Wang F, Wang J, Liu D, et al. Normalizing genes for real-time polymerase chain reaction in epithelial and nonepithelial cells of mouse small intestine[J]. Analytical biochemistry, 2010, 399(2): 211-217.
  7. 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Jeong J K, Kang M H, Gurunathan S, et al. Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)[J]. BMC research notes, 2014, 7(1): 675.
  8. 8.0 8.1 8.2 8.3 8.4 Veazey K J, Golding M C. Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells[J]. PloS one, 2011, 6(11): e27592.
  9. 9.0 9.1 9.2 9.3 Matoušková P, Bártíková H, Boušová I, et al. Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity[J]. PLoS One, 2014, 9(1): e86033.
  10. 10.0 10.1 10.2 Gong Z K, Wang S J, Huang Y Q, et al. Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development[J]. Molecular genetics and genomics, 2014, 289(6): 1157-1169.
  11. 11.0 11.1 11.2 Sirakov M, Borra M, Cambuli F M, et al. Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells[J]. Molecular biotechnology, 2013, 54(3): 930-938.
  12. 12.0 12.1 12.2 12.3 12.4 Thomas K C, Zheng X F, Suarez F G, et al. Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle[J]. PloS one, 2014, 9(2): e88653.

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