Caenorhabditis elegans
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Contents
Description
- Caenorhabditis elegans, a free-living nematode that feeds on soil microorganisms, is the most abundant soil-dwelling invertebrate that occupied a key position in terrestrial ecosystem by influencing energy transfer and nutrient cycling. It is a simple multicellular eukaryote with its genome first completely sequenced and its cell lineage well described. As a simple and welldefined genetic model, C. elegans has gained increasing popularity among scientists to study the molecular mechanism of emerging materials[1][2].
- Common Name: Worm,Nematode
- NCBI Taxonomy
Nanotoxicity
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| tba-1[1] | Tubulin, alpha family member |
|
NA |
|
NA | 60 | SYBR |
| Y45F10D.4[1] | Putative iron-sulfur cluster assembly enzyme |
|
NA |
|
NA | 60 | SYBR |
| pmp-3[1] | Peroxisomal membrane protein related |
|
NA |
|
NA | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- Comparative ΔCt method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Yanqiong Zhang
- Email: Panx@ecu.edu
- Institution: Department of Biology, East Carolina University, Greenville, North Carolina, United States of America
Citation Statistics
Cited by 69 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3 Zhang Y, Chen D, Smith M A, et al. Selection of reliable reference genes in Caenorhabditis elegans for analysis of nanotoxicity[J]. PloS one, 2012, 7(3): e31849.
- ↑ Murray AR, Kisin E, Leonard SS, Young SH, Kommineni C, et al. Oxidative stress and inflammatory response in dermal toxicity of single-walled carbon nanotubes. Toxicology 257: 161–171.
