Difference between revisions of "Actinobacillus suis"
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| Line 80: | Line 80: | ||
*'''Name''': Janet I MacInnes | *'''Name''': Janet I MacInnes | ||
*'''Email''': macinnes@uoguelph.ca | *'''Email''': macinnes@uoguelph.ca | ||
| − | *''' | + | *'''Institution''': Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, N1G 2W1 Guelph, Ontario, Canada |
===Citation Statistics=== | ===Citation Statistics=== | ||
Revision as of 08:45, 17 August 2017
Contents
Description
- Actinobacillus suis is a Gram negative facultative anaerobe which is a frequent member of the normal microbiome of swine tonsils of the soft palate. It is also an important pathogen in pigs of all ages, where it can cause septicaemia and sequelae such as meningitis, arthritis, and pleuropneumonia [1][2].
- Common Name: Unknown
- NCBI Taxonomy
Different Growth Conditions
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| pyk[1] | Pyruvate kinase |
|
AFU19347 |
|
NA | 60 | SYBR |
| rpoB[1] | DNA-directed RNA polymerase subunit β |
|
AFU20083 |
|
NA | 60 | SYBR |
| proS[1] | Prolyl-tRNAsynthetase |
|
AFU19772 |
|
NA | 60 | SYBR |
| gyrA[1] | DNA gyrase subunit A |
|
AFU18440 |
|
NA | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- ΔCt approach method && Related Reference
- BestKeeper method && Related Reference
- Ref-Finder method && Related Reference
Contact
- Name: Janet I MacInnes
- Email: macinnes@uoguelph.ca
- Institution: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road East, N1G 2W1 Guelph, Ontario, Canada
Citation Statistics
Cited by 4 (Based on Google Scholar [2017-08-10])
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Bujold AR, MacInnes JI. Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis. BMC Res Notes. 2015 Mar 18;8:86. doi: 10.1186/s13104-015-1045-8. PubMed PMID: 25884823; PubMed Central PMCID: PMC4369107.
- ↑ Kernaghan S, Bujold AR, MacInnes JI. The microbiome of the soft palate of swine. Anim Health Res Rev. 2012;13:110–20.
