Difference between revisions of "Anguilla anguilla"
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* Universal reference gene | * Universal reference gene | ||
Revision as of 14:35, 22 June 2017
Contents
Description
- The European eel (Anguilla anguilla) is a catadromous teleost, which exhibits a complex life cycle with spawning in the ocean and growing up in continental waters. After spawning in the Sargasso Sea, within the North Atlantic gyre, eel larvae follow the Atlantic currents towards continental waters of Europe and North Africa, where they metamorphose into glass eels.
- After reaching coastal waters, estuaries, lagoons, rivers and lakes, they turn into yellow (sexually immature) eels and remain for several years in continental waters until they undergo a pre-pubertal secondary metamorphosis called silvering and transform into silver eels. The silver eels migrate back to spawning grounds, where they eventually mate and die.
- Eel landings have decreased rapidly throughout Europe since the 1960s (ICES, 2014). The same applies for the coastal waters of Estonia, where eel landings have declined by over 90% compared to the beginning of the 2000s. However, eel landings in freshwaters have remained steady for the past 5 years (averaging 15 tonnes/year; ICES, 2015), as these populations rely completely on stocking.[1] [2].
Silvering
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| L13[1] | Ribosomal protein L13 |
|
eeel2_c1081 |
|
180 | 60 | SYBR |
| ARP[1] | Acidic ribosomal protein |
|
AY763793 |
|
107 | 60 | SYBR |
Moleculer Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- Ref-Finder method && Related Reference
- ΔCt approach method && Related Reference
Contact
- Name: Elena Fabbri
- Email: silvia.franzellitti@unibo.it
- Institution: University of Bologna, Department of Biological, Geological, and Environmental Sciences, via Selmi 3, 40100 Bologna Italy
Citation Statistics
Cited by 3 (Based on Google Scholar [2017-06-16])
References
- ↑ 1.0 1.1 1.2 Franzellitti S, Kiwan A, Valbonesi P, Fabbri E (2015) Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla). Sci Rep 5, 16966.
- ↑ Silm M, Bernotas P, Haldna M, Jarvalt A, Noges T (2017) Age and growth of European eel, Anguilla Anguilla (Linnaeus, 1758), in Estonian lakes. Journal of Applied Ichthyology 33, 236-241.
