Difference between revisions of "Apis mellifera"
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| − | ==Description== | + | =='''Description'''== |
| − | [[File: | + | [[File:Apis mellifera.png|right|200px|link=Apis mellifera]] |
| − | * | + | * '''''Apis mellifera''''' is the most important managed pollinator species in the world, its pollination services are estimated at contributing $14 billion annually to the economy. Honeybees are critical for agricultural ecosystems, but are exposed to many biotic stressors including bacteria, viruses and fungi. Honey bee is also a highly social insect and represents a key model for social behavior studies. It is also the first hymenopteran insect to have its genome fully sequenced<ref name="ref1"/><ref name="ref2"/>. |
| − | + | * <font color=blue>'''Common Name:'''</font> '''Honeybee''' | |
| + | * [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=7460 <font color=blue>'''NCBI Taxonomy'''</font>] | ||
=='''''Bacterial Challenge'''''== | =='''''Bacterial Challenge'''''== | ||
| − | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
| Line 12: | Line 13: | ||
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
| − | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
! Detection | ! Detection | ||
|- | |- | ||
| − | |align="center"| | + | |align="center"| Act<ref name="ref1"/> |
| − | |align="center"| | + | |align="center"| Actin |
| | | | ||
| − | * | + | * Head |
| + | * Bacterial challenge with ''Escherichis coli'' | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB023025 '''AB023025'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB023025 '''AB023025'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 32: | Line 34: | ||
|align="center"| GAPDH | |align="center"| GAPDH | ||
| | | | ||
| − | * | + | * Head |
| + | * Bacterial challenge with ''Escherichis coli'' | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_393605 '''XM_393605'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_393605 '''XM_393605'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 44: | Line 47: | ||
|align="center"| RPS18 | |align="center"| RPS18 | ||
| | | | ||
| − | * | + | * Head |
| + | * Bacterial challenge with ''Escherichis coli'' | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_625101 '''XM_625101'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_625101 '''XM_625101'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 54: | Line 58: | ||
|} | |} | ||
| − | === | + | ===Molecular Types=== |
*mRNA | *mRNA | ||
===Evaluation Methods=== | ===Evaluation Methods=== | ||
| Line 65: | Line 69: | ||
*'''Institution''': Laboratory of Zoophysiology, Department of Biochemistry, Physiology and Microbiology, Faculty of Science, Ghent University, Belgium | *'''Institution''': Laboratory of Zoophysiology, Department of Biochemistry, Physiology and Microbiology, Faculty of Science, Ghent University, Belgium | ||
| − | ==Citation Statistics== | + | ===Citation Statistics=== |
| − | Cited by ''' | + | Cited by [https://scholar.google.com/scholar?cites=1788032570351657373&as_sdt=2005&sciodt=0,5&hl=en '''150'''] (Based on Google Scholar [2017-09-01]) |
=='''References'''== | =='''References'''== | ||
| Line 77: | Line 81: | ||
</ref> | </ref> | ||
</references> | </references> | ||
| + | =='''Categories'''== | ||
| + | [[Category:Animals]] | ||
| + | [[Category:mRNA]] | ||
| − | [[Category: | + | [[Category:SYBR]] |
| + | [[Category:ACT]] [[Category:GAPDH]] [[Category:RPS]][[Category:Biotic Stress]] [[Category:Pathological conditions]] [[Category:Bacterial Infection]] | ||
| + | [[Category:geNorm]] | ||
| + | [[Category:NormFinder]] | ||
| + | [[Category:BestKeeper]] | ||
Latest revision as of 06:13, 1 September 2017
Contents
Description
- Apis mellifera is the most important managed pollinator species in the world, its pollination services are estimated at contributing $14 billion annually to the economy. Honeybees are critical for agricultural ecosystems, but are exposed to many biotic stressors including bacteria, viruses and fungi. Honey bee is also a highly social insect and represents a key model for social behavior studies. It is also the first hymenopteran insect to have its genome fully sequenced[1][2].
- Common Name: Honeybee
- NCBI Taxonomy
Bacterial Challenge
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| Act[1] | Actin |
|
AB023025 |
|
155 | 61 | SYBR |
| GAPDH[1] | GAPDH |
|
XM_393605 |
|
203 | 61 | SYBR |
| RPS18 [1] | RPS18 |
|
XM_625101 |
|
149 | 61 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Bieke Scharlaken
- Email: Bieke.Scharlaken@UGent.be
- Institution: Laboratory of Zoophysiology, Department of Biochemistry, Physiology and Microbiology, Faculty of Science, Ghent University, Belgium
Citation Statistics
Cited by 150 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3 Bieke Scharlaken, Dirk C. de Graaf, Karen Goossens, Marleen Brunain, Luc J. Peelman, Frans J. Jacobs. Reference Gene Selection for Insect Expression Studies Using Quantitative Real-Time PCR: The Head of the Honeybee, Apis mellifera, After a Bacterial Challenge[J]. Journal of Insect Science, 2008, 8(33):1-10.
- ↑ Mao W, Schuler M A, Berenbaum M R. Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(22):8842-6.
