Difference between revisions of "Apis mellifera"
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| − | Scharlaken | + | Bieke Scharlaken, Dirk C. de Graaf, Karen Goossens, Marleen Brunain, Luc J. Peelman, Frans J. Jacobs. Reference Gene Selection for Insect Expression Studies Using Quantitative Real-Time PCR: The Head of the Honeybee, Apis mellifera, After a Bacterial Challenge[J]. Journal of Insect Science, 2008, 8(33):1-10. |
| + | </ref> | ||
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| + | Mao W, Schuler M A, Berenbaum M R. Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(22):8842-6. | ||
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Revision as of 06:28, 17 June 2017
Contents
Description
- The western honey bee Apis mellifera is the most important managed pollinator species in the world; in the United States, its pollination services are estimated at contributing $14 billion annually to the economy.
- Honeybees (Apis mellifera), are critical for agricultural ecosystems, but are exposed to many biotic stressors including bacteria, viruses and fungi.[1] [2].
Bacterial Challenge
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| actin[1] | actin |
|
AB023025 |
|
155 | 61 | SYBR |
| GAPDH[1] | GAPDH |
|
XM_393605 |
|
203 | 61 | SYBR |
| RPS18 [1] | RPS18 |
|
XM_625101 |
|
149 | 61 | SYBR |
Moleculer Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Bieke Scharlaken
- Email: Bieke.Scharlaken@UGent.be
- Institution: Laboratory of Zoophysiology, Department of Biochemistry, Physiology and Microbiology, Faculty of Science, Ghent University, Belgium
References
- ↑ 1.0 1.1 1.2 1.3 Bieke Scharlaken, Dirk C. de Graaf, Karen Goossens, Marleen Brunain, Luc J. Peelman, Frans J. Jacobs. Reference Gene Selection for Insect Expression Studies Using Quantitative Real-Time PCR: The Head of the Honeybee, Apis mellifera, After a Bacterial Challenge[J]. Journal of Insect Science, 2008, 8(33):1-10.
- ↑ Mao W, Schuler M A, Berenbaum M R. Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(22):8842-6.
