Difference between revisions of "Arachis hypogaea"
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===Evaluation Methods=== | ===Evaluation Methods=== | ||
* [https://genorm.cmgg.be/ '''geNorm method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference'''] | * [https://genorm.cmgg.be/ '''geNorm method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference'''] | ||
Revision as of 08:23, 17 August 2017
Contents
Description
- Arachis hypogaea L., an allotetraploid species, is one of the Wve most important oilseed crops cultivated worldwide. It is an important food and oil crop, is grown throughout the tropics and subtropics. Cultivated peanut is an allotetraploid (AABB genome). Its ancestral species are most likely the diploid Arachis duranensis and Arachis ipaensis, which contributed the A and B subgenomes, respectively Peanut seed contains 50% oil, of which 80% consists of oleic acids and linoleic acids[1][2].
- Common Name: Peanut
- NCBI Taxonomy
Different Tissues
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| ADH3[1] | Alcohol dehydrogenase |
|
EG529529 |
|
143 | 60 | SYBR |
| G6PD[1] | Glucose-6-phospahate 1- dehydrogenase |
|
EG030635 |
|
151 | 60 | SYBR |
| ELF1B[1] | Elongation factor 1B |
|
EE126175 |
|
153 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Pooja Bhatnagar-Mathur
- Email: p.bhatnagar@cgiar.org
- Institution: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, India
Citation Statistics
Cited by 30 (Based on Google Scholar [2017-06-29])
Different Developmental Stages & Abiotic Stress
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| UKN1[2] | Hypothetical protein |
|
EG028875 |
|
102 | 58~60 | SYBR |
| TUA5[2] | Alpha tubulin |
|
GO264294 |
|
94 | 58~60 | SYBR |
| UKN2[2] | Hypothetical protein |
|
GO334386 |
|
89 | 58~60 | SYBR |
| ACT11[2] | Actin 11 |
|
GO264911 |
|
196 | 58~60 | SYBR |
| EF1b[2] | Elongation factor 1b |
|
GO339841 |
|
66 | 58~60 | SYBR |
| HDC[2] | Nuclear helicase |
|
EE123564 |
|
196 | 58~60 | SYBR |
Molecular types
- mRNA
Evaluation Methods
Contact
- Name: Shanlin Yu
- Email: yshanlin1956@163.com
- Institution: Shandong Peanut Research Institute, Qingdao 266100, People’s Republic of China
Citation Statistics
Cited by 59 (Based on Google Scholar [2017-08-10])
References
- ↑ 1.0 1.1 1.2 1.3 Reddy D S, Bhatnagar-Mathur P, Cindhuri K S, et al. Evaluation and validation of reference genes for normalization of quantitative real-time PCR based gene expression studies in peanut[J]. PloS one, 2013, 8(10): e78555.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Chi X, Hu R, Yang Q, et al. Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR[J]. Molecular Genetics and Genomics, 2012, 287(2): 167-176.
