Bactrocera dorsalis

Description

Bactrocera dorsalis is a species of tephritid fruit fly that is endemic to Southeast Asia, but has also been introduced to Hawaii, the Mariana Islands and Tahiti. It is one of the major pest species in the genus Bactrocera with a broad host range of cultivated and wild fruits.
Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon^[1] ^[2].

Gene Symbol	Gene Name	Application Scope	Accession Number	Primer	Size [bp]	Tm [℃]	Detection
α-TUB^[1]	alpha tubulin	female flies males files fat body	GU269902	F:CGCATTCATGGTTGATAACG R:GGGCACCAAGTTAGTCTGGA	184	60	SYBR
ACT3^[1]	clone BdA3	males files	L12255	F:GGTCGGTATGGGACAGAAGG R: CTCACGATTGGCTTTTGGAT	220	60	SYBR
ACT5^[1]	clone BdA5	female flies	L12256	F:CAACTCACCCGCAATGTATG R:CGCTCAGCAGTGGTTGTAAA	237	60	SYBR
ACT1^[1]	clone BdA1	fat body	L12253	F:AGCGTGAAATCGTGAGGGA R:GACAAGACCGAGTTGGCATA	286	60	SYBR
ACT2 ^[1]	clone BdA2	both males and females	L12254	F:GTGTGATGGTTGGTATGGGA R:GGCTGGGGAGTTGAAGGTTT	269	60	SYBR

Name: Jin-Jun Wang
Email: jjwang7008@yahoo.com
Institution: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Shen G M, Jiang H B, Wang X N, et al. Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)[J]. BMC molecular biology, 2010, 11(1): 76.
↑ Cheng D, Guo Z, Riegler M, et al. Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)[J]. Microbiome, 2017, 5(1): 13.