Brassica napus

Description

Oilseed rape (Brassica napus) is one of the most agriculturally important crops in the world, because of its large oilseed production (approximately 64 million metric tons (MMT) worldwide in 2012), with China producing about 14 MMT and Canada, the European Union, India, and Australia being the other major contributors.
During cultivation of oilseed rape, it will be exposed to various adverse environmental conditions, including abiotic and biotic stresses, such as drought, high-salinity, and pathogens, which result in production losses.
For instance, yield losses in the crop as high as 24 % caused by Sclerotinia sclerotiorum have been reported in Australia. In recent years, more and more researchers have focused on gene expression studies in this crop under various exogenous stimulations to understand its defense mechanisms.

Gene Symbol	Gene Name	Application Scope	Accession Number	Primer	Size [bp]	Tm [℃]	Detection
ACT7^[1]^[2]	Actin7	Heavy metal (cr 6+) stress Methyl jasmonate stress Abscisic acid stress	Bra028615	F:GcTGaccGTaTGaGcaaaG R:aaGaTGGaTGGacccGac	182	85.4	SYBR
SAND^[1]	SanD-family protein	Cold stress	Bra000494	F:GcTGaaGGTGGaTTGcGTG R:GGaGTTTcTGGTaTGcTcGGTaT	244	82.7	SYBR
UBC21^[1]	Ubiquitin-conjugating enzyme 21	Drought stress Sclerotinia sclerotiorum stress	EV086936	F:ccTcTGcaGccTccTcaaGT R:caTaTcTccccTGTcTTGaaaTGc	77	78.8	SYBR
PP2A^[1]	Protein phosphatase 2a subunit a3	Salt stress Drought stress Methyl jasmonate stress Sclerotinia sclerotiorum stress	Bra012474	F:GTcaacaaTccGcacTaccTaca R:caaccacGacGGGaaGaaac	112	81.4	SYBR
ZNF^[1]	Zinc finger protein	Salicylic acid stress	Bra032656	F:TTcaGGcGGTTTaTGGc R:TcGGGcTTTGcGTaGTT	168	83.4	SYBR
TIP41^[1]	TIP41-like Protein	Salt stress Heavy metal (cr 6+) stress Methyl jasmonate stress Abscisic acid stress Sclerotinia sclerotiorum stress	Bra011516	F:TGaaGaGcaGaTTGaTTTGGcT R:acacTccaTTGTcaGccaGTT	101	78.5	SYBR
F-box^[1]	F-box/kelch-repeat protein	Cold stress Salicylic acid stress	Bra006317	F:GaGaTaaGTcGcTTccTaccG R:TGTTcccaTTGcccTGTG	184	81.9	SYBR

Name: Zheng Wang
Email: xltan@ujs.edu.cn
Institute: Institute of life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, People’s Republic of china

Cited by 24 (Based on Google Scholar [2017-06-16])

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Wang Z, Chen Y, Fang H, et al. Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions[J]. Molecular genetics and genomics, 2014, 289(5): 1023-1035.
↑ Artico S, Nardeli SM, Brilhante O, Grossi-de-Sa MF, Alves-Ferreira M (2010) Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data. BMC Plant Biol 10:49