Difference between revisions of "Brassica oleracea"

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(Created page with "==Description== right|227px| * The genus Brassica belongs to the Brassicaceae family. Many Brassica species are important vegetable crops, among...")
 
 
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==Description==
 
==Description==
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[[File:REFqPCR2014009-1.jpg|right|227px|]]
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[[File:Brassica oleracea.png|right|200px|]]
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* The genus Brassica belongs to the Brassicaceae family. Many Brassica species are important vegetable crops, among which the cole crop, Brassica oleracea. This species has a great diversity of morphotypes (broccoli, cauliflower, collard greens, brussels sprouts) and among them, one of the most important taxonomic variety is viridis (forage kale).
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* '''''Brassica oleracea''''' belongs to the genus Brassica of Brassicaceae family. It is an important vegetable crop which has a great diversity of morphotypes (broccoli, cauliflower, collard greens, brussels sprouts). It is the most diverse species within the Brassica genus, being important for human nutrition, with multiple cultivars that can be classified by the morphology of their edible structures<ref name="ref1"/><ref name="ref2"/>.
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* B. oleracea is a close relative of the model plant A. thaliana. Both share a common ancestor from which they have diverged 15–20 million years ago. Parts of their corresponding genomes are equivalently organized despite numerous chromosomal rearrangements during respective evolution of the two genera.
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* <font color=blue>'''Common Name:'''</font> '''Cabbage''', '''Broccoli''', '''Cauliflower''', '''Kale'''
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* Brassica oleracea is the most diverse species within the Brassica genus, being important for human nutrition, with multiple cultivars that can be classified by the morphology of their edible structures.  <ref name="ref1"/> <ref name="ref2"/>.
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=3712 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Abiotic Stresses'''''==
 
=='''''Abiotic Stresses'''''==
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===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
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! style="width=25%　font-size:9pt　"|Application Scope  
 
! style="width=25%　font-size:9pt　"|Application Scope  
 
! Accession Number  
 
! Accession Number  
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! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|align="center"| Ubiquitin
 
|align="center"| Ubiquitin
 
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* Universial reference gene
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* Leaves
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* Drought and cold abiotic stresses
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW695428 '''GW695428''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW695428 '''GW695428''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Ribosomal protein L18
 
|align="center"| Ribosomal protein L18
 
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* Universial reference gene
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* Leaves
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* Drought and cold abiotic stresses
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW695428 '''GW695428''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW695428 '''GW695428''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Elongation factor 1-alpha
 
|align="center"| Elongation factor 1-alpha
 
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* Universial reference gene
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* Leaves
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* Drought and cold abiotic stresses
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW694601 '''GW694601''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GW694601 '''GW694601''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|}
 
|}
  
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===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
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===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Email''': sylvain.dumez@univ-lille2.fr
 
*'''Email''': sylvain.dumez@univ-lille2.fr
 
*'''Institution''': Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Nord de France, EA 4483, Lille 2, B.P. 83, 59006 Lille Cedex, France
 
*'''Institution''': Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Nord de France, EA 4483, Lille 2, B.P. 83, 59006 Lille Cedex, France
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===Citation Statistics===
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Cited by [https://scholar.google.com/scholar?cites=11651543811632989386&as_sdt=2005&sciodt=0,5&hl=en '''10'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
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[[Category:Plants]][[Category:mRNA]][[Category:MESA Blue]][[Category:EF1α]] [[Category:RPL]] [[Category:Ubiquitin]][[Category:Abiotic Stress]][[Category:geNorm]]
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[[Category:NormFinder]]
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[[Category:BestKeeper]]

Latest revision as of 08:01, 1 September 2017

Description

Brassica oleracea.png
  • Brassica oleracea belongs to the genus Brassica of Brassicaceae family. It is an important vegetable crop which has a great diversity of morphotypes (broccoli, cauliflower, collard greens, brussels sprouts). It is the most diverse species within the Brassica genus, being important for human nutrition, with multiple cultivars that can be classified by the morphology of their edible structures[1][2].
  • Common Name: Cabbage, Broccoli, Cauliflower, Kale
  • NCBI Taxonomy

Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
Ubiq[1] Ubiquitin
  • Leaves
  • Drought and cold abiotic stresses
 GW695428
  • F:TCCAGGACAAAGAAGGGATTCC
  • R:AGGGCTCTCAAGCTGGGTTCAA
 166 60 MESA Blue
Rpl18[1] Ribosomal protein L18
  • Leaves
  • Drought and cold abiotic stresses
 GW695428
  • F:TCCCTGGCCAAGCTGGTTCG
  • R:CAGGGCCAACTGATCAAATGTGA
 201 60 MESA Blue
EF1α[1] Elongation factor 1-alpha
  • Leaves
  • Drought and cold abiotic stresses
 GW694601
  • F:GGAACTTCCCAGGCAGACTGTGC
  • R:TCAAAACGGGCCGCAGAGAAT
 191 60 MESA Blue

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Sylvain Dumez
  • Email: sylvain.dumez@univ-lille2.fr
  • Institution: Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Lille Nord de France, EA 4483, Lille 2, B.P. 83, 59006 Lille Cedex, France

Citation Statistics

Cited by 10 (Based on Google Scholar [2017-09-01])

References

  1. ↑ 1.0 1.1 1.2 1.3 Brulle F, Bernard F, Vandenbulcke F, Cuny D, Dumez S (2014) Identification of suitable qPCR reference genes in leaves of Brassica oleracea under abiotic stresses. Ecotoxicology 23, 459-471.
  2. ↑ Tortosa M, Velasco P, Afonso D, et al. (2017) Characterization of a Spanish Brassica oleracea collection by using molecular and biochemical markers. Scientia Horticulturae 219, 344-350.

Categories

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