Difference between revisions of "Bubalus bubalis"

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=='''Description'''==
 
=='''Description'''==
[[File:REFqPCR2017001-1.jpg|right|227px|]]
+
[[File:Bubalus bubalis.png|right|200px|link=Bubalus bubalis]]
* The water buffalo or domestic asian water buffalo ('''''Bubalus bubalis''''') is an important dairy animal in  Asian  countries.  It is a large bovid originating in South Asia, Southeast Asia, and China. Today, it is also found in Europe, Australia, South America and some African countries.
+
* '''''Bubalus bubalis''''' is an important dairy animal in  Asian  countries, which is a large bovid originating in South Asia, Southeast Asia, and China. Today, it is also found in Europe, Australia, South America and some African countries.It contributes significantly to the agricultural economy and food security of countries on the Indian subcontinent and in Southeast Asia, through  milk,  meat,  hides,  and draught power. Buffalo milk is considered to be economical for the production of casein, caseinates, whey protein concentrate, and fat-rich dairy products<ref name="ref1"/> <ref name="ref2"/>.
* It contributes significantly to the agricultural economy and food security of countries on the Indian subcontinent and in Southeast Asia.  It  contributes  through  milk,  meat,  hides,  and draught power.
+
* <font color=blue>'''Common Name:'''</font> '''Domestic asian water buffalo ''',''' Water buffalo'''
* Buffalo milk is considered to be economical for the production of casein, caseinates, whey protein concentrate, and fat-rich dairy products. <ref name="ref1"/> <ref name="ref2"/> .
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=89462 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Spermatozoa'''''==
 
=='''''Spermatozoa'''''==
===Reference Genes===
+
===Internal Control Genes===
 +
 
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:AGCTAAAGGAGTTTCCACCGT
 
* F:AGCTAAAGGAGTTTCCACCGT
* R:AGCTAAAGGAGTTTCCACCGT
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* R:GGTGGTAGGTCAATCCGGG
 
|align="center"|  207
 
|align="center"|  207
 
|align="center"| 56~64
 
|align="center"| 56~64
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|-
 
|-
 
|align="center"| ATP2B4<ref name="ref1"/>
 
|align="center"| ATP2B4<ref name="ref1"/>
|align="center"| ATPase, Caþþ transporting,plasma membrane 4
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|align="center"| ATPase, Ca2+ transporting,plasma membrane 4
 
|
 
|
 
* Fresh and frozen-thawed spermatozoa
 
* Fresh and frozen-thawed spermatozoa
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GTTTGGCTGCACAGTGATGG
 
* F:GTTTGGCTGCACAGTGATGG
* R:GTTTGGCTGCACAGTGATGG
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* R:TCCCAGATAGGCCTTCCACA
 
|align="center"|  143
 
|align="center"|  143
 
|align="center"| 56~64
 
|align="center"| 56~64
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|}
 
|}
  
===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
 
===Evaluation Methods===  
 
===Evaluation Methods===  
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*'''Institution''': Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India
 
*'''Institution''': Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India
  
==Citation Statistics==
+
===Citation Statistics===
Cited by '''0''' (Based on Google Scholar [2017-06-01])
+
Cited by [https://scholar.google.com/scholar?q=related:I2I3Nvx7qnIJ:scholar.google.com/&hl=en&as_sdt=0,5 '''0'''] (Based on Google Scholar [2017-09-01])
 +
 
 +
=='''''Mammary Epithelial Cells'''''==
 +
===Reference Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 +
|-
 +
! Gene Symbol 
 +
! Gene Name
 +
! style="width=25% font-size:9pt "|Application Scope
 +
! Accession Number
 +
! Primers (5'-3')<br>[Forward/Reverse]
 +
! Size [bp]
 +
! Tm [℃]
 +
! Detection
 +
|-
 +
|align="center"| RPS9<ref name="ref3"/>
 +
|align="center"|  ribosomal protein S9
 +
|
 +
*Mammary epithelial cells during different phases of lactation
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001101152 '''NM_001101152 ''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CCTCGACCAAGAGCTGAAG
 +
* R:CCTCCAGACCTCACGTTTGTTC
 +
|align="center"| NA
 +
|align="center"| NA
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| RPS23<ref name="ref3"/>
 +
|align="center"| ribosomal protein S23
 +
|
 +
*Mammary epithelial cells during different phases of lactation
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/gene/?term=NP_001029862.1 '''NP_001029862.1''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CCCAATGATGGTTGCTTGAA
 +
* R:CGGACTCCAGGAATGTCACC
 +
|align="center"| NA
 +
|align="center"| NA
 +
|align="center"| SYBR
 +
|}
 +
===Molecular Types===
 +
* mRNA
 +
 
 +
===Evaluation Methods===
 +
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 +
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 +
===Contact===
 +
*'''Name''': B.P. Mishra
 +
*'''Email''': bpmishra_1@hotmail.com
 +
*'''Institution''': National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=16520028400192373503&as_sdt=2005&sciodt=0,5&hl=en '''18'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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Jul;86(7):1495-502. doi: 10.2527/jas.2007-0321. Epub 2008 Mar 14. PubMed PMID:
 
Jul;86(7):1495-502. doi: 10.2527/jas.2007-0321. Epub 2008 Mar 14. PubMed PMID:
 
18344309.
 
18344309.
 +
</ref>
 +
<ref name="ref3">
 +
Yadav, Poonam, et al. Identification of suitable housekeeping genes for expression analysis in mammary epithelial cells of buffalo (Bubalus bubalis) during lactation cycle. Livestock Science 147.1 (2012): 72-76.
 
</ref>
 
</ref>
 
</references>
 
</references>
 +
 +
=='''Categories'''==
 +
[[Category:Animals]]
 +
[[Category:mRNA]]
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[[Category:SYBR]]
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[[Category:ATP2B4]] [[Category:GLUT5]]
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[[Category:Specific Tissue]]
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[[Category:Different Developmental Stages]]
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[[Category:RPS]]
 +
[[Category:Multiple Projects]]
 +
[[Category:geNorm]]
 +
[[Category:NormFinder]]
 +
[[Category:BestKeeper]]
 +
[[Category:Delta Ct]]
 +
[[Category:RefFinder]]

Latest revision as of 06:44, 1 September 2017

Description

Bubalus bubalis.png
  • Bubalus bubalis is an important dairy animal in Asian countries, which is a large bovid originating in South Asia, Southeast Asia, and China. Today, it is also found in Europe, Australia, South America and some African countries.It contributes significantly to the agricultural economy and food security of countries on the Indian subcontinent and in Southeast Asia, through milk, meat, hides, and draught power. Buffalo milk is considered to be economical for the production of casein, caseinates, whey protein concentrate, and fat-rich dairy products[1] [2].
  • Common Name: Domestic asian water buffalo , Water buffalo
  • NCBI Taxonomy

Spermatozoa

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
GLUT5[1] Solute carrier family 2 (facilitated glucose/fructose transporter), member 5
  • Fresh and frozen-thawed spermatozoa
NM_001101042.2
  • F:AGCTAAAGGAGTTTCCACCGT
  • R:GGTGGTAGGTCAATCCGGG
207 56~64 SYBR
ATP2B4[1] ATPase, Ca2+ transporting,plasma membrane 4
  • Fresh and frozen-thawed spermatozoa
NM_001172594
  • F:GTTTGGCTGCACAGTGATGG
  • R:TCCCAGATAGGCCTTCCACA
143 56~64 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: S.K. Bhure
  • Email: sdbhure@rediffmail.com
  • Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India

Citation Statistics

Cited by 0 (Based on Google Scholar [2017-09-01])

Mammary Epithelial Cells

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
RPS9[3] ribosomal protein S9
  • Mammary epithelial cells during different phases of lactation
NM_001101152
  • F:CCTCGACCAAGAGCTGAAG
  • R:CCTCCAGACCTCACGTTTGTTC
NA NA SYBR
RPS23[3] ribosomal protein S23
  • Mammary epithelial cells during different phases of lactation
NP_001029862.1
  • F:CCCAATGATGGTTGCTTGAA
  • R:CGGACTCCAGGAATGTCACC
NA NA SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: B.P. Mishra
  • Email: bpmishra_1@hotmail.com
  • Institution: National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, India

Citation Statistics

Cited by 18 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 Ashish S, Bhure SK, Harikrishna P, Ramteke SS, Muhammed Kutty VH, Shruthi N, Ravi Kumar GV, Manish M, Ghosh SK, Mihir S. Identification and evaluation of reference genes for accurate gene expression normalization of fresh and frozen-thawed spermatozoa of water buffalo (Bubalus bubalis). Theriogenology. 2017 Apr 1;92:6-13. doi: 10.1016/j.theriogenology.2017.01.006. Epub 2017 Jan 8. PubMed PMID: 28237344.
  2. Vijh RK, Tantia MS, Mishra B, Bharani Kumar ST. Genetic relationship and diversity analysis of Indian water buffalo (Bubalus bubalis). J Anim Sci. 2008 Jul;86(7):1495-502. doi: 10.2527/jas.2007-0321. Epub 2008 Mar 14. PubMed PMID: 18344309.
  3. 3.0 3.1 Yadav, Poonam, et al. Identification of suitable housekeeping genes for expression analysis in mammary epithelial cells of buffalo (Bubalus bubalis) during lactation cycle. Livestock Science 147.1 (2012): 72-76.

Categories