Difference between revisions of "Camellia sinensis"
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|align="center"| Polypyrimidine tract-binding protein | |align="center"| Polypyrimidine tract-binding protein | ||
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| − | * | + | * Sampled at different time of a day |
| + | * Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots | ||
| + | * Cold treatment | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GAAC01052498.1 '''GAAC01052498.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nucest/GAAC01052498.1 '''GAAC01052498.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 33: | Line 35: | ||
|align="center"| Elongation factor 1 alpha | |align="center"| Elongation factor 1 alpha | ||
| | | | ||
| − | * | + | * Sampled at different time of a day |
| + | * Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots | ||
| + | * Cold treatment | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA280301.1 '''KA280301.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA280301.1 '''KA280301.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 45: | Line 49: | ||
|align="center"| SAND family protein | |align="center"| SAND family protein | ||
| | | | ||
| − | * | + | * Sampled at different time of a day |
| + | * Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots | ||
| + | * Cold treatment | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KM057790.1 '''KM057790'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KM057790.1 '''KM057790'''] | ||
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|align="center"| Clathrin adaptor complex subunit | |align="center"| Clathrin adaptor complex subunit | ||
| | | | ||
| − | * | + | * Sampled at different time of a day |
| + | * Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots | ||
| + | * Cold treatment | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA291473.1 '''KA291473.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA291473.1 '''KA291473.1'''] | ||
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|align="center"| Ubiquitin-conjugating enzyme | |align="center"| Ubiquitin-conjugating enzyme | ||
| | | | ||
| − | * | + | * Sampled at different time of a day |
| + | * Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots | ||
| + | * Cold treatment | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA281185.1 '''KA281185.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/KA281185.1 '''KA281185.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Revision as of 15:18, 4 July 2017
Contents
Description
- Camellia sinensis is the most consumed beverage in the world aside from water, and has many beneficial health effects. It is an important cash crop, and is grown commercially in about 30 different countries. It is manufactured by drying fresh tea leaves and contains characteristic polyphenolic compounds, (–)-epigallocatechin-3-gallate (EGCG), (–)-epigallocatechin (EGC), (–)-epicatechin-3-gallate (ECG) and (–)-epicatechin (EC). These compounds are commonly known as catechins[1][2].
- Common Name: Green tea
- NCBI Taxonomy
Various Experimental Treatments
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| CsPTB1[1] | Polypyrimidine tract-binding protein |
|
GAAC01052498.1 |
|
107 | 60 | SYBR |
| CsEF1[1] | Elongation factor 1 alpha |
|
KA280301.1 |
|
110 | 60 | SYBR |
| CsSAND1[1] | SAND family protein |
|
KM057790 |
|
109 | 60 | SYBR |
| CsCLATHRIN1[1] | Clathrin adaptor complex subunit |
|
KA291473.1 |
|
129 | 60 | SYBR |
| CsUBC1[1] | Ubiquitin-conjugating enzyme |
|
KA281185.1 |
|
124 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- ΔCt approach method && Related Reference
Contact
- Name: Bin Xiao
- Email: xiaobin2093@sohu.com
- Institution: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China
Citation Statistics
Cited by 31 (Based on Google Scholar [2017-06-16])
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Hao X, Horvath DP, Chao WS, et al. (2014) Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze). Int J Mol Sci 15, 22155-22172.
- ↑ Yang CS, Landau JM (2000) Effects of tea consumption on nutrition and health. Journal of Nutrition 130, 2409-2412.
