Camellia sinensis

Description

• Tea is the most consumed beverage in the world aside from water, and has many beneficial health effects. Tea plant (Camellia sinensis), is an important cash crop, and is grown commercially in about 30 different countries.
• Green tea is manufactured by drying fresh tea leaves. It contains characteristic polyphenolic compounds, (–)-epigallocatechin-3-gallate (EGCG),3 (–)-epigallocatechin (EGC), (–)-epicatechin-3-gallate (ECG) and (–)-epicatechin (EC). These compounds are commonly known as catechins.
• A typical tea beverage, prepared in a proportion of 1 g leaf to 100 mL water in a 3-min brew, usually contains 250–350 mg tea solids, comprised of 30–42% catechins and 3–6% caffeine. .

Various Experimental Treatments

Reference Genes

Gene Symbol	Gene Name	Application Scope	Accession Number	Primers (5'-3') [Forward/Reverse]	Size [bp]	Tm [℃]	Detection
CsPTB1	Polypyrimidine tract-binding protein	Universal reference gene	GAAC01052498.1	F:TGACCAAGCACACTCCACACTATCG R:TGCCCCCTTATCATCATCCACAA	107	60	SYBR
CsEF1	Elongation factor 1 alpha	Universal reference gene	KA280301.1	F:TTGGACAAGCTCAAGGCTGAACG R:ATGGCCAGGAGCATCAATGACAGT	110	60	SYBR
CsSAND1	SAND family protein	Universal reference gene	KM057790	F:TCCAATTGCCCCCTTAATGACTCA R:GTAAGGGCAGGCAAACACCAGGTA	109	60	SYBR
CsCLATHRIN1	Clathrin adaptor complex subunit	Universal reference gene	KA291473.1	F:TAGAGCGGGTAGTGGAGACCTCGTT R:TACCAAAGCCGGCTCGTATGAGATT	129	60	SYBR
CsUBC1	Ubiquitin-conjugating enzyme	Universal reference gene	KA281185.1	F:TGCTGGTGGGGTTTTTCTTGTTACC R:AAGGCATATGCTCCCATTGCTGTTT	124	60	SYBR

Molecular Types

• mRNA

Evaluation Methods

• geNorm method && Related Reference
• NormFinder method && Related Reference
• BestKeeper method && Related Reference
• ΔCt approach method && Related Reference

Contact

• Name: Bin Xiao
• Email: xiaobin2093@sohu.com
• Institution: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China

Citation Statistics

Cited by 31 (Based on Google Scholar [2017-06-16])