Camellia sinensis

Description

• Camellia sinensis is the most consumed beverage in the world aside from water, and has many beneficial health effects. It is an important cash crop, and is grown commercially in about 30 different countries. It is manufactured by drying fresh tea leaves and contains characteristic polyphenolic compounds, (–)-epigallocatechin-3-gallate (EGCG), (–)-epigallocatechin (EGC), (–)-epicatechin-3-gallate (ECG) and (–)-epicatechin (EC). These compounds are commonly known as catechins.
• Common Name: Green tea
• NCBI Taxonomy

Various Experimental Treatments

Internal Control Genes

Gene Symbol	Gene Name	Application Scope	Accession Number	Primers (5'-3') [Forward/Reverse]	Size [bp]	Tm [℃]	Detection
CsPTB1	Polypyrimidine tract-binding protein	Sampled at different time of a day Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots Cold treatment	GAAC01052498.1	F:TGACCAAGCACACTCCACACTATCG R:TGCCCCCTTATCATCATCCACAA	107	60	SYBR
CsEF1	Elongation factor 1 alpha	Sampled at different time of a day Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots Cold treatment	KA280301.1	F:TTGGACAAGCTCAAGGCTGAACG R:ATGGCCAGGAGCATCAATGACAGT	110	60	SYBR
CsSAND1	SAND family protein	Sampled at different time of a day Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots Cold treatment	KM057790	F:TCCAATTGCCCCCTTAATGACTCA R:GTAAGGGCAGGCAAACACCAGGTA	109	60	SYBR
CsCLATHRIN1	Clathrin adaptor complex subunit	Sampled at different time of a day Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots Cold treatment	KA291473.1	F:TAGAGCGGGTAGTGGAGACCTCGTT R:TACCAAAGCCGGCTCGTATGAGATT	129	60	SYBR
CsUBC1	Ubiquitin-conjugating enzyme	Sampled at different time of a day Leaves, whole flowers at half boom, shoots, five-month-old stems, axillary buds and roots Cold treatment	KA281185.1	F:TGCTGGTGGGGTTTTTCTTGTTACC R:AAGGCATATGCTCCCATTGCTGTTT	124	60	SYBR

Molecular Types

• mRNA

Evaluation Methods

• geNorm method && Related Reference
• NormFinder method && Related Reference
• BestKeeper method && Related Reference
• ΔCt approach method && Related Reference

Contact

• Name: Bin Xiao
• Email: xiaobin2093@sohu.com
• Institution: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China

Citation Statistics

Cited by 31 (Based on Google Scholar [2017-09-01])

Leaf development & Hormonal Treatment

Internal Control Genes

Gene Symbol	Gene Name	Application Scope	Accession Number	Primers (5'-3') [Forward/Reverse]	Size [bp]	Tm [℃]	Detection
CsTBP	TATA-box binding protein gene	Leaf development and metabolism Hormonal stimuli	AT5G09810 (Arabidopsis homolog gene)	F:GGCGGATCAAGTGTTGGAAGGGAG R:ACGCTTGGGATTGTATTCGGCATTA	166	85	SYBR
CsTIP41	Tap42-interacting protein of 41 kDa gene	Leaf development and metabolism	AT4G34270 (Arabidopsis homolog gene)	F:TGGAGTTGGAAGTGGACGAGACCGA R:CTCTGGAAAGTGGGATGTTTGAAGC	176	87	SYBR

Molecular Types

• mRNA

Evaluation Methods

• geNorm method && Related Reference
• NormFinder method && Related Reference
• BestKeeper method && Related Reference

Contact

• Name: Jing Zhuang
• Email: zhuangjing@njau.edu.cn
• Institution: Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

Citation Statistics

Cited by 18 (Based on Google Scholar [2017-09-01])

Biotic Stress & Hormonal Treatment

Internal Control Genes

Gene Symbol	Gene Name	Application Scope	Accession Number	Primers (5'-3') [Forward/Reverse]	Size [bp]	Tm [℃]	Detection
miR159a	miR159a	Bud to the fifth leaf Methyl jasmonate (MeJA) Treatment Salicylic acid (SA) Treatment Abscisic acid (ABA)	NA	F:CGCGCTTGGATTGAAGGG R:NA	NA	60	SYBR
5S rRNA	5S rRNA	Different organs	NA	F:GCGATCATACCAGCACCAATG R:CAACACAAGGACTTCCTAGGG	NA	60	SYBR
miR6149	miR6149	Leaves were attacked by Ectropis oblique and U4	NA	F:GCGCGATACGCACCTGAAT R:NA	NA	60	SYBR
miR5368n	miR5368n	Methyl jasmonate (MeJA) Treatment Salicylic acid (SA) Treatment Abscisic acid (ABA) Treatment	NA	F:GCGGAGATACCACTCTGG R:NA	NA	60	SYBR

Molecular Types

• miRNA

Evaluation Methods

• geNorm method && Related Reference
• NormFinder method && Related Reference

Contact

• Name: Chaoling Wei
• Email: weichl@ahau.edu.cn;
• Institution: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China

Citation Statistics

Cited by 3 (Based on Google Scholar [2017-09-01])