Difference between revisions of "Cicer arietinum"

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==Description==
 
==Description==
  
[[File:REFqPCR2016014-1.JPG|right|227px|]]
+
[[File:Cicer arietinum.png|right|200px|link=Cicer arietinum]]
* Chickpea is an important food legume of the semi-arid tropical (SAT) regions of the world, known to be a nutraceutical (or health benefiting food) because of its high nutritional value. Despite growing demand and high yield potential, chickpea yield is unstable and productivity is stagnant at unacceptably low levels due to constraints such as abiotic stresses (drought, heat, cold and high-salinity) and biotic stresses (Ascochyta blight, Fusarium wilt and pod borer).
+
* '''''Cicer arietinum''''' is an important food legume of the semi-arid tropical (SAT) regions of the world, known to be a health benefiting food because of its high nutritional value. Despite growing demand and high yield potential, its unstable yield and productivity is stagnant at unacceptably low levels due to constraints such as abiotic stresses (drought, heat, cold and high-salinity) and biotic stresses (Ascochyta blight, Fusarium wilt and pod borer). Cultivation with sodium metavanadate, sodium chloride or sodium sulfate in the early stage can increase its concentration of formononetin, ononin, daidzein and biochanin A glucoside contents <ref name="ref1"/> <ref name="ref2"/> <ref name="ref3"/>.
* chickpeas germinated in presence of sodium metavanadate increased 32% and 11% the formononetin and biochanin A contents, respectively. Also a combination of sodium chloride and sodium sulfate, used during chickpea soaking before 4 germination days, increased the concentration of ononin, daidzein and biochanin A glucoside by 71%, 11% and 13%, respectively, compared with the germinated control. <ref name="ref1"/> <ref name="ref2"/> <ref name="ref3"/>.
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* <font color=blue>'''Common Name:'''</font> '''Chickpea'''
 +
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=3827 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Across Cultivated and Wild Species'''''==
 
=='''''Across Cultivated and Wild Species'''''==
===Reference Genes===
+
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
+
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:TGGAGCCCAATTACAAAAGC
 
* F:TGGAGCCCAATTACAAAAGC
* R:TGGAGCCCAATTACAAAAGC
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* R:TTTGAAGCCAAAGAGGCAAC
 
|align="center"|  138  
 
|align="center"|  138  
 
|align="center"| 62
 
|align="center"| 62
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:ACAACGATACCAGGGTGTTACC
 
* F:ACAACGATACCAGGGTGTTACC
* R:ACAACGATACCAGGGTGTTACC
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* R:TCTCCCATGATGCCTTTAACTC
 
|align="center"|  116  
 
|align="center"|  116  
 
|align="center"| 62
 
|align="center"| 62
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GTTGTACTTCGGGAGAGTTGCT
 
* F:GTTGTACTTCGGGAGAGTTGCT
* R:GTTGTACTTCGGGAGAGTTGCT
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* R:GGAGCTTCTGGCTTATGATGCT
 
|align="center"|  115  
 
|align="center"|  115  
 
|align="center"| 62
 
|align="center"| 62
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:TCACAGGTTGTGATGGAGTCTG
 
* F:TCACAGGTTGTGATGGAGTCTG
* R:TCACAGGTTGTGATGGAGTCTG
+
* R:CCTCAAATCTTGTTGGGGTGTC
 
|align="center"|  135  
 
|align="center"|  135  
 
|align="center"| 62
 
|align="center"| 62
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|}
 
|}
  
===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
 +
 
===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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===Citation Statistics===
 
===Citation Statistics===
Cited by '''9''' (Based on Google Scholar [2017-06-01])
+
Cited by [https://scholar.google.com/scholar?cites=17132640821263345199&as_sdt=2005&sciodt=0,5&hl=en '''9'''] (Based on Google Scholar [2017-09-01])
  
 
=='''Different Developmental Stages & Abiotic Stress'''==
 
=='''Different Developmental Stages & Abiotic Stress'''==
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
+
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|align="center"| Elongation factor 1-alpha
 
|align="center"| Elongation factor 1-alpha
 
|
 
|
*Various organs developmental stages;
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*Various organs developmental stages
 
*Various stress conditions
 
*Various stress conditions
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AJ004960 '''AJ004960''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AJ004960 '''AJ004960''']  
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|}
 
|}
  
===Moleculer Type===
+
===Molecular Types===
 
* mRNA
 
* mRNA
 +
 
===Evaluation Methods===
 
===Evaluation Methods===
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Email''': mjainanid@gmail.com  
 
*'''Email''': mjainanid@gmail.com  
 
*'''Institution''': National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110 067, India
 
*'''Institution''': National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110 067, India
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=7460091868871764874&as_sdt=2005&sciodt=0,5&hl=en '''104'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
 
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=='''Categories'''==
 
[[Category:Plants]]
 
[[Category:Plants]]
[[Category:mRNA]] 
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[[Category:mRNA]][[Category:SYBR]]
[[Category:SYBR]]
 
 
[[Category:ABCT]] [[Category:EF1α]] [[Category:G6PD]] [[Category:GAPDH]] [[Category:HSP90]] [[Category:TIP41]] [[Category:UCP]]
 
[[Category:ABCT]] [[Category:EF1α]] [[Category:G6PD]] [[Category:GAPDH]] [[Category:HSP90]] [[Category:TIP41]] [[Category:UCP]]
 +
[[Category:Cultivated & Wild Species]]  [[Category:Abiotic Stress]]  [[Category:Different Developmental Stages]]
 +
 +
[[Category:geNorm]]
 +
[[Category:NormFinder]]
 +
[[Category:RefFinder]]

Latest revision as of 09:06, 1 September 2017

Description

Cicer arietinum.png
  • Cicer arietinum is an important food legume of the semi-arid tropical (SAT) regions of the world, known to be a health benefiting food because of its high nutritional value. Despite growing demand and high yield potential, its unstable yield and productivity is stagnant at unacceptably low levels due to constraints such as abiotic stresses (drought, heat, cold and high-salinity) and biotic stresses (Ascochyta blight, Fusarium wilt and pod borer). Cultivation with sodium metavanadate, sodium chloride or sodium sulfate in the early stage can increase its concentration of formononetin, ononin, daidzein and biochanin A glucoside contents [1] [2] [3].
  • Common Name: Chickpea
  • NCBI Taxonomy

Across Cultivated and Wild Species

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
UCP[1] Uncharacterized conserved protein
  • Across different genotypes
XM_004505295
  • F:TGGAGCCCAATTACAAAAGC
  • R:TTTGAAGCCAAAGAGGCAAC
138 62 SYBR
G6PD[1] Glucose-6-phosphate 1-dehydrogenase
  • Across different genotypes
XM_004515773
  • F:ACAACGATACCAGGGTGTTACC
  • R:TCTCCCATGATGCCTTTAACTC
116 62 SYBR
TIP41[1] Tonoplast intrinsic proteins -like protein
  • Abiotic stress conditions
XM_004496854
  • F:GTTGTACTTCGGGAGAGTTGCT
  • R:GGAGCTTCTGGCTTATGATGCT
115 62 SYBR
ABCT[1] ATP-binding cassette transporter
  • Universal reference gene
XM_004505589
  • F:TCACAGGTTGTGATGGAGTCTG
  • R:CCTCAAATCTTGTTGGGGTGTC
135 62 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Pooja Bhatnagar-Mathur
  • Email: p.bhatnagar@cgiar.org
  • Institution: Genetic Transformation Laboratory, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Telangana, India

Citation Statistics

Cited by 9 (Based on Google Scholar [2017-09-01])

Different Developmental Stages & Abiotic Stress

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
EF1α[2] Elongation factor 1-alpha
  • Various organs developmental stages
  • Various stress conditions
AJ004960
  • F:TCCACCACTTGGTCGTTTTG
  • R:CTTAATGACACCGACAGCAACAG
64 60 SYBR
HSP90[2] Heat shock protein 90
  • Various organs developmental stages
GR406804
  • F:GCAGCATGGCTGGTTACATGT
  • R:GCAGCATGGCTGGTTACATGT
63 60 SYBR
GAPDH[2] Glyceraldehyde-3-phosphate dehydrogenase
  • Various stress conditions
AJ010224
  • F:CCAAGGTCAAGATCGGAATCA
  • R:CAAAGCCACTCTAGCAACCAAA
65 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Mukesh Jain
  • Email: mjainanid@gmail.com
  • Institution: National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110 067, India

Citation Statistics

Cited by 104 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 1.3 1.4 Reddy DS, Bhatnagar-Mathur P, Reddy PS, et al. (2016) Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species. PLoS One 11, e0148451.
  2. 2.0 2.1 2.2 2.3 Garg R, Sahoo A, Tyagi A K, et al. Validation of internal control genes for quantitative gene expression studies in chickpea (Cicer arietinum L.)[J]. Biochemical and biophysical research communications, 2010, 396(2): 283-288.
  3. Guardado-Felix D, Serna-Saldivar SO, Cuevas-Rodriguez EO, Jacobo-Velazquez DA, Gutierrez-Uribe JA (2017) Effect of sodium selenite on isoflavonoid contents and antioxidant capacity of chickpea (Cicer arietinum L.) sprouts. Food Chem 226, 69-74.

Categories