Description
Salt and Heat Stress
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| EF1[1]
|
Elongation factor 1
|
- nitrogen starvation
- salt
- heat stress
|
GW484749.1
|
- F:CTGTCCTTGATTGCCACACTTCT
- R:CTTGGGCTCCTTCTCAAGCTC
|
NA
|
60
|
SYBR
|
| EF1a[1]
|
Elongation factor 1-alpha
|
- nitrogen starvation
- salt
- heat stress
|
GT708303.1
|
- F:AAGGGAGCTTCCAGCTTTACCT
- R:TGTGAGAGGTGTGGCAGTCAA
|
NA
|
60
|
SYBR
|
| GAPDH[1]
|
glyceraldehyde-3-phosphate-dehydrogenase
|
- nitrogen starvation
- salt
- heat stress
|
GW488886.1
|
- F:AGGCTGTTGGGAAAGTTCTTC
- R:ACTGTTGGAACTCGGAATGC
|
NA
|
60
|
SYBR
|
| MDH[1]
|
Malate dehydrogenase
|
- nitrogen starvation
- salt
- heat stress
|
GW464198.1
|
- F:CCTGATGTCAACCACGCAACT
- R:GTGGTTATGAACTCTCCATTCAACC
|
NA
|
60
|
SYBR
|
| UBQ10[1]
|
Polyubiquitin 10
|
- nitrogen starvation
- salt
- heat stress
|
GT697658.1
|
- F:CAGACCAGCAGAGGCTGATT
- R:AGAACCAAGTGAAGGGTGGA
|
NA
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Kenia de Carvalho
- Email: : keniadecarvalho@gmail.com
- Institute: Programa de Po´s-Graduac ¸a ˜o em Agronomia (Produc ¸a ˜o Vegetal),Departamento de Fitotecnia e Fitossanitarismo, Universidade Federal do Parana ´, Curitiba, PR CEP 80035-050, Brazil
Citation Statistics
Cited by 22 (Based on Google Scholar [2017-06-16])
Different Air CO2 Supply & Temperature
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| MDH[2]
|
Malate Dehydrogenase
|
|
GW464198.1
|
- F:CCTGATGTCAACCACGCAACT
- R:CCTGATGTCAACCACGCAACT
|
100
|
60
|
SYBR
|
| ACT [2]
|
Actin
|
|
GT000704
|
- F:AAGCTTGCCTATGTGGCTCTTG
- R:AAGCTTGCCTATGTGGCTCTTG
|
100
|
60
|
SYBR
|
| S15[2]
|
40S ribosomal protein S15
|
|
GR987196
|
- F:CAATAGGGGCTTGAAGAGG
- R:CAATAGGGGCTTGAAGAGG
|
146
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: José C. Ramalho
- Email: cochichor@isa.ulisboa.pt
- Institution: Plant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Linking Landscape, Environment, Agriculture and Food, Departmento de Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, Universidade de Lisboa (ULisboa), Oeiras, Portugal
References
- ↑ 1.0 1.1 1.2 1.3 1.4
de Carvalho K, Bespalhok Filho J C, Dos Santos T B, et al. Nitrogen starvation, salt and heat stress in coffee (Coffea arabica L.): identification and validation of new genes for qPCR normalization[J]. Molecular biotechnology, 2013, 53(3): 315-325.
- ↑ 2.0 2.1 2.2 Cite error: Invalid
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no text was provided for refs named ref2
