Difference between revisions of "Cucumis sativus"

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(Created page with "==Description== =='''''Different Nitrogen Nutrition'''''== ===Reference Genes=== {|class="wikitable sortable" style="font-size:10pt; width:100%" |- ! Gene Symbol ! Gene Name...")
 
 
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==Description==
 
==Description==
=='''''Different Nitrogen Nutrition'''''==
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===Reference Genes===
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[[File:Cucumis sativus.png|right|200px|link=Cucumis sativus]]
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* '''''Cucumber, Cucumis sativus L.''''' is one of the most important cultivated vegetable crops, ranking 4th in quantity of world vegetable production. Three fourths of this is produced in China where both the area harvested and the quantity produced increase year by year. Many different traits relating to the quality of cucumber fruit have been reported, including the length, weight, and diameter of fruit, uniform immature fruit color, glossy fruit skin, warty fruit, and yellow fruit flesh<ref name="ref1"/><ref name="ref2"/>.
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* <font color=blue>'''Common Name:'''</font> '''Cucumber'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=3659 <font color=blue>'''NCBI Taxonomy'''</font>]
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=='''''Various Nutrition Conditions'''''==
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|align="center"| SYBR
 
|align="center"| SYBR
 
|-
 
|-
|align="center"| EFa<ref name="ref1"/>
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|align="center"| EF1α<ref name="ref1"/>
 
|align="center"| Elongation factor 1-alpha
 
|align="center"| Elongation factor 1-alpha
 
|
 
|
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|}
 
|}
  
===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
 +
 
===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Email''': anka.warzybok@biol.uni.wroc.pl
 
*'''Email''': anka.warzybok@biol.uni.wroc.pl
 
*'''Institution''': Wrocław University, Institute of Experimental Biology, Department of Plant Molecular Physiology, Wroclaw, Poland
 
*'''Institution''': Wrocław University, Institute of Experimental Biology, Department of Plant Molecular Physiology, Wroclaw, Poland
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===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=5511009913552534712&as_sdt=2005&sciodt=0,5&hl=en '''25'''] (Based on Google Scholar [2017-09-01])
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 +
=='''Different Tissues & Hormones Treatment & abiotic stresses'''==
 +
 +
===Internal Control Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
 +
! Gene Symbol 
 +
! Gene Name
 +
! style="width=25% font-size:9pt "|Application Scope
 +
! Accession Number
 +
! Primers (5'-3')<br>[Forward/Reverse]
 +
! Size [bp]
 +
! Tm [℃]
 +
! Detection
 +
|-
 +
|align="center"|  EF1α<ref name="ref2"/>
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|align="center"| Elongation factor 1-α
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|
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*Different tissues
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*Abiotic stress
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/EF446145 '''EF446145''']
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|nowrap style="font-size:9pt"|
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* F:ACTGTGCTGTCCTCATTATTG
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* R:AGGGTGAAAGCAAGAAGAGC
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|align="center"| 98
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|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| UBI-ep<ref name="ref2"/>
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|align="center"| Ubiquitin extension protein
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|
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*Different tissues
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*Different hormones treatments
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AY372537 '''AY372537''']
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|nowrap style="font-size:9pt"|
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* F:CACCAAGCCCAAGAAGATC
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* R:TAAACCTAATCACCACCAGC
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|align="center"| 220
 +
|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| TUA<ref name="ref2"/>
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|align="center"| α-Tubulin
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|
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*Different tissues
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*Different hormones treatments
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AJ715498 '''AJ715498''']
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|nowrap style="font-size:9pt"|
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* F:ACGCTGTTGGTGGTGGTAC
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* R:GAGAGGGGTAAACAGTGAATC
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|align="center"| 106
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|align="center"| 60
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|align="center"| SYBR
 +
|}
 +
 +
===Molecular Types===
 +
* mRNA
 +
 +
===Evaluation Methods===
 +
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 +
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 +
* [http://www.gene-quantification.com/bestkeeper.html '''BestKeeper method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15127793 '''Related Reference''']
 +
===Contact===
 +
*'''Name''': Jinfeng Chen
 +
*'''Email''': jfchen@njau.edu.cn
 +
*'''Institution''': State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=17001919382398512439&as_sdt=2005&sciodt=0,5&hl=en '''215'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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<ref name="ref1">
 
<ref name="ref1">
 
Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition[J]. PLoS One, 2013, 8(9): e72887.
 
Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition[J]. PLoS One, 2013, 8(9): e72887.
 +
</ref>
 +
<ref name="ref2">
 +
Wan H, Zhao Z, Qian C, et al. Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber[J]. Analytical biochemistry, 2010, 399(2): 257-261.
 
</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
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[[Category:Plants]] [[Category:mRNA]] [[Category:SYBR]]
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[[Category:CACS]] [[Category:EF1α]] [[Category:EF1α]] [[Category:F-box]] [[Category:TIP41]] [[Category:Tubulin]] [[Category:Ubiquitin]]
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[[Category:Hormone Treatment]]  [[Category:Different Tissues]]  [[Category:Abiotic Stress]]  [[Category:Various  Nutrition Conditions]]
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[[Category:geNorm]]
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[[Category:NormFinder]]
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[[Category:BestKeeper]]

Latest revision as of 09:41, 1 September 2017

Description

Cucumis sativus.png
  • Cucumber, Cucumis sativus L. is one of the most important cultivated vegetable crops, ranking 4th in quantity of world vegetable production. Three fourths of this is produced in China where both the area harvested and the quantity produced increase year by year. Many different traits relating to the quality of cucumber fruit have been reported, including the length, weight, and diameter of fruit, uniform immature fruit color, glossy fruit skin, warty fruit, and yellow fruit flesh[1][2].
  • Common Name: Cucumber
  • NCBI Taxonomy

Various Nutrition Conditions

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
CACS[1] AP-2 complex subunit mu-1
  • Cucumber organs under nitrogen-related stress
GT035008
  • F:GTGCTTTCTTTCTGGAATGC
  • R:TGAACCTCGTCAAATTTACACA
158 60 SYBR
TIP41[1] TIP41-like family protein
  • Cucumber organs under nitrogen-related stress
GW881871
  • F:CAACAGGTGATATTGGATTATGATTATAC
  • R:GCCAGCTCATCCTCATATAAG
221 60 SYBR
F-box[1] F-box/kelch-repeat protein
  • Cucumber organs under nitrogen-related stress
GW881870
  • F:GGTTCATCTGGTGGTCTT
  • R:CTTTAAACGAACGGTCAGTCC
166 60 SYBR
EF1α[1] Elongation factor 1-alpha
  • Cucumber organs under nitrogen-related stress
EF446145
  • F:ACTTTATCAAGAACATGATTAC
  • R:TTCCTTCACAATTTCATCG
230 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Anna Warzybok
  • Email: anka.warzybok@biol.uni.wroc.pl
  • Institution: Wrocław University, Institute of Experimental Biology, Department of Plant Molecular Physiology, Wroclaw, Poland

Citation Statistics

Cited by 25 (Based on Google Scholar [2017-09-01])

Different Tissues & Hormones Treatment & abiotic stresses

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
EF1α[2] Elongation factor 1-α
  • Different tissues
  • Abiotic stress
EF446145
  • F:ACTGTGCTGTCCTCATTATTG
  • R:AGGGTGAAAGCAAGAAGAGC
98 60 SYBR
UBI-ep[2] Ubiquitin extension protein
  • Different tissues
  • Different hormones treatments
AY372537
  • F:CACCAAGCCCAAGAAGATC
  • R:TAAACCTAATCACCACCAGC
220 60 SYBR
TUA[2] α-Tubulin
  • Different tissues
  • Different hormones treatments
AJ715498
  • F:ACGCTGTTGGTGGTGGTAC
  • R:GAGAGGGGTAAACAGTGAATC
106 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jinfeng Chen
  • Email: jfchen@njau.edu.cn
  • Institution: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China

Citation Statistics

Cited by 215 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 1.3 1.4 Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition[J]. PLoS One, 2013, 8(9): e72887.
  2. 2.0 2.1 2.2 2.3 Wan H, Zhao Z, Qian C, et al. Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber[J]. Analytical biochemistry, 2010, 399(2): 257-261.

Categories