Description
- Cucumber, Cucumis sativus L., is one of the most important cultivated vegetable crops, ranking 4th in quantity of world vegetable production. Three fourths of this is produced in China where both the area harvested and the quantity produced increase year by year. Many different traits relating to the quality of cucumber fruit have been reported, including the length, weight, and diameter of fruit, uniform immature fruit color, glossy fruit skin, warty fruit, and yellow fruit flesh[1] [2].
Different Nitrogen Nutrition
Reference Genes
Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
CACS[1]
|
AP-2 complex subunit mu-1
|
- Cucumber organs under nitrogen-related stress
|
GT035008
|
- F:GTGCTTTCTTTCTGGAATGC
- R:TGAACCTCGTCAAATTTACACA
|
158
|
60
|
SYBR
|
TIP41[1]
|
TIP41-like family protein
|
- Cucumber organs under nitrogen-related stress
|
GW881871
|
- F:CAACAGGTGATATTGGATTATGATTATAC
- R:GCCAGCTCATCCTCATATAAG
|
221
|
60
|
SYBR
|
F-box[1]
|
F-box/kelch-repeat protein
|
- Cucumber organs under nitrogen-related stress
|
GW881870
|
- F:GGTTCATCTGGTGGTCTT
- R:CTTTAAACGAACGGTCAGTCC
|
166
|
60
|
SYBR
|
EF1α[1]
|
Elongation factor 1-alpha
|
- Cucumber organs under nitrogen-related stress
|
EF446145
|
- F:ACTTTATCAAGAACATGATTAC
- R:TTCCTTCACAATTTCATCG
|
230
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Anna Warzybok
- Email: anka.warzybok@biol.uni.wroc.pl
- Institution: Wrocław University, Institute of Experimental Biology, Department of Plant Molecular Physiology, Wroclaw, Poland
Citation Statistics
Cited by 24 (Based on Google Scholar [2017-06-16])
Different Tissues & Hormones Treatment & abiotic stresses
Reference Genes
Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
EF1α[2]
|
Elongation factor 1-α
|
- Different tissues;*abiotic stress treatments
|
EF446145
|
- F:ACTGTGCTGTCCTCATTATTG
- R:AGGGTGAAAGCAAGAAGAGC
|
98
|
60
|
SYBR
|
UBI-ep[2]
|
Ubiquitin extension protein
|
- Different tissues;
- Treated with different hormones
|
AY372537
|
- F:CACCAAGCCCAAGAAGATC
- R:TAAACCTAATCACCACCAGC
|
220
|
60
|
SYBR
|
TUA[2]
|
α-Tubulin
|
- Different tissues;
- Treated with different hormones
|
AJ715498
|
- F:ACGCTGTTGGTGGTGGTAC
- R:GAGAGGGGTAAACAGTGAATC
|
106
|
60
|
SYBR
|
Moleculer Type
Evaluation Methods
Contact
- Name: Jinfeng Chen
- Email: jfchen@njau.edu.cn
- Institution: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China
References
- ↑ 1.0 1.1 1.2 1.3 1.4
Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition[J]. PLoS One, 2013, 8(9): e72887.
- ↑ 2.0 2.1 2.2 2.3
Wan H, Zhao Z, Qian C, et al. Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber[J]. Analytical biochemistry, 2010, 399(2): 257-261.