Cucumis sativus

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Description

Different Nitrogen Nutrition

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
CACS[1] AP-2 complex subunit mu-1
  • Cucumber organs under nitrogen-related stress
 GT035008
  • F:GTGCTTTCTTTCTGGAATGC
  • R:TGAACCTCGTCAAATTTACACA
 158 60 SYBR
TIP41[1] TIP41-like family protein
  • Cucumber organs under nitrogen-related stress
 GW881871
  • F:CAACAGGTGATATTGGATTATGATTATAC
  • R:GCCAGCTCATCCTCATATAAG
 221 60 SYBR
F-box[1] F-box/kelch-repeat protein
  • Cucumber organs under nitrogen-related stress
 GW881870
  • F:GGTTCATCTGGTGGTCTT
  • R:CTTTAAACGAACGGTCAGTCC
 166 60 SYBR
EFa[1] Elongation factor 1-alpha
  • Cucumber organs under nitrogen-related stress
 EF446145
  • F:ACTTTATCAAGAACATGATTAC
  • R:TTCCTTCACAATTTCATCG
 230 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Anna Warzybok
  • Email: anka.warzybok@biol.uni.wroc.pl
  • Institution: Wrocław University, Institute of Experimental Biology, Department of Plant Molecular Physiology, Wroclaw, Poland

References

  1. ↑ 1.0 1.1 1.2 1.3 Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition[J]. PLoS One, 2013, 8(9): e72887.
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