Daucus carota

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Description

REFqPCR2015002-1.jpg
  • Carrot is the most widely grown member of Apiaceae family. Its progenitor, wild Daucus carota L., is a plant commonly occurring in the temperate climatic zones. To date, a range molecular tools facilitating genome analysis in context of evolutionary history of wild and cultivated carrot have been developed, i.e., DArT, SSR, and SNP markers and a set of ca. 30 resequenced genomes.
  • The carrot (Daucus carota subsp. sativus) is a root vegetable, usually orange in colour, though purple, black, red, white, and yellow cultivars exist. Carrots are a domesticated form of the wild carrot, Daucus carota, native to Europe and southwestern Asia. The plant probably originated in Persia and originally cultivated for its leaves and seeds. The most commonly eaten part of the plant is the taproot, although the greens are sometimes eaten as well. The domestic carrot has been selectively bred for its greatly enlarged, more palatable, less woody-textured taproot.
  • Daucus carota, whose common names include wild carrot, bird's nest, bishop's lace, and Queen Anne's lace (North America), is a white, flowering plant in the family Apiaceae, native to temperate regions of Europe and southwest Asia, and naturalized to North America and Australia. [1] [2].

Different Experimental Systems

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GAPDH[1] glyceraldehyde 3-phosphate dehydrogenase
  • Tap root secondary growth
AY491512
  • F:GGGAGGTGCAAAGAAAGTTATCA
  • R:GGGAGGTGCAAAGAAAGTTATCA
79 60 SYBR
5.8S rRNA[1] D.carota 5.8S, 18S and 25S ribosomal RNA genes and ITS regions
  • Carrot tap root secondary growth
  • Somatic embryogenesis realization experiment
X17534
  • F:AATGACTCTCGGCAACGGATAT
  • R:AATGACTCTCGGCAACGGATAT
73 60 SYBR
25S rRNA[1] D.carota 5.8S, 18S and 25S ribosomal RNA genes and ITS regions
  • Somatic embryogenesis realization experiment
X17534
  • F:AGTCGGGTTGTTTGGGAATG
  • R:AGTCGGGTTGTTTGGGAATG
67 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Hélia Cardoso
  • Email: hcardoso@uevora.pt
  • Institution: EU Marie Curie Chair, ICAAM, Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA, Universidade de Évora, Ap. 94, 7006-554 Évora, Portugal

Citation Statistics

Cited by 11 (Based on Google Scholar [2017-06-01])

References

  1. 1.0 1.1 1.2 1.3 Campos MD, Frederico AM, Nothnagel T, Arnholdt-Schmitt B, Cardoso H (2015) Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.). Scientia Horticulturae 186, 115-123.
  2. Stelmach K, Macko-Podgórni A, Machaj G, Grzebelus D (2017) Miniature Inverted Repeat Transposable Element Insertions Provide a Source of Intron Length Polymorphism Markers in the Carrot (Daucus carota L.). Frontiers in Plant Science 8.