Difference between revisions of "Delomys sublineatus"

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=='''Description'''==
 
=='''Description'''==
 
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[[File:Delomys sublineatus.png|right|200px|link=Delomys sublineatus]]
[[File:Delomys sublineatus-1.jpg|right|327px|]]
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*'''''Delomys sublineatus''''' is listed as Least Concern in view of its wide distribution, presumed large population, and because it is unlikely to be declining at nearly the rate required to qualify for listing in a threatened category; also it occurs in several protected areas and is locally common.  a rodent used as indicator species in conservation studies of the endangered Brazilian rainforest, suitable primer sets are more problematic to find than in model species<ref name="ref1"/><ref name="ref2"/> .
*Pallid Atlantic Forest Rat (Delomys sublineatus), a rodent used as indicator species in conservation studies of the endangered Brazilian rainforest, suitable primer sets are more problematic to find than in model species. Out of nine tested primer sets designed for the fully sequenced Mus musculus,  five could be used for the establishment of a proper running SYBR-Green assay and validation of their constant expression<ref name="ref1"/> <ref name="ref2"/> .  
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* <font color=blue>'''Common Name:'''</font> '''Pallid atlantic forest rat'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=89131 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Liver Samples'''''==
 
=='''''Liver Samples'''''==
===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
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|align="center"| Succinate dehydrogenase complex, subunit A
 
|align="center"| Succinate dehydrogenase complex, subunit A
 
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* Universal reference genes
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* Live-trapped in the Brazilian coastal Atlantic Forest
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* Adult male or females without pregnant or lactating
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361627 '''GU361627''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361627 '''GU361627''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Calnexin
 
|align="center"| Calnexin
 
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* Universal reference genes
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* Live-trapped in the Brazilian coastal Atlantic Forest
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* Adult male or females without pregnant or lactating
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361628 '''GU361628''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361628 '''GU361628''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Phosphoglycerate kinase 1
 
|align="center"| Phosphoglycerate kinase 1
 
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* Universal reference genes
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* Live-trapped in the Brazilian coastal Atlantic Forest
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* Adult male or females without pregnant or lactating
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361632 '''GU361632''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361632 '''GU361632''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Beta-actin  
 
|align="center"| Beta-actin  
 
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* Universal reference genes
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* Live-trapped in the Brazilian coastal Atlantic Forest
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* Adult male or females without pregnant or lactating
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361629 '''GU361629''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361629 '''GU361629''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Gamma-actin 1, cytoplasmatic
 
|align="center"| Gamma-actin 1, cytoplasmatic
 
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* Universal reference genes
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* Live-trapped in the Brazilian coastal Atlantic Forest
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* Adult male or females without pregnant or lactating
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361630 '''GU361630''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GU361630 '''GU361630''']  
 
|nowrap style="font-size:9pt"|
 
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*'''Institution''': Leibniz Institute for Zoo and Wildlife Research (IZW), Evolutionary Genetics, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany
 
*'''Institution''': Leibniz Institute for Zoo and Wildlife Research (IZW), Evolutionary Genetics, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany
 
===Citation Statistics===
 
===Citation Statistics===
Cited by '''13''' (Based on Google Scholar [2017-06-16])
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Cited by [https://scholar.google.com/scholar?cites=11330997337831670564&as_sdt=2005&sciodt=0,5&hl=en '''13'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
 
[[Category:Animals]]
 
[[Category:Animals]]
 
[[Category:mRNA]] [[Category:SYBR]]
 
[[Category:mRNA]] [[Category:SYBR]]
 
[[Category:ACT]] [[Category:Canx]] [[Category:PGK]] [[Category:SDHA]]
 
[[Category:ACT]] [[Category:Canx]] [[Category:PGK]] [[Category:SDHA]]
 
[[Category:Specific Tissue]]
 
[[Category:Specific Tissue]]
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[[Category:geNorm]]
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[[Category:NormFinder]]
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[[Category:BestKeeper]]

Latest revision as of 07:06, 1 September 2017

Description

Delomys sublineatus.png
  • Delomys sublineatus is listed as Least Concern in view of its wide distribution, presumed large population, and because it is unlikely to be declining at nearly the rate required to qualify for listing in a threatened category; also it occurs in several protected areas and is locally common. a rodent used as indicator species in conservation studies of the endangered Brazilian rainforest, suitable primer sets are more problematic to find than in model species[1][2] .
  • Common Name: Pallid atlantic forest rat
  • NCBI Taxonomy

Liver Samples

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Sdha[1] Succinate dehydrogenase complex, subunit A
  • Live-trapped in the Brazilian coastal Atlantic Forest
  • Adult male or females without pregnant or lactating
GU361627

NA

152 55 SYBR
Canx[1] Calnexin
  • Live-trapped in the Brazilian coastal Atlantic Forest
  • Adult male or females without pregnant or lactating
GU361628

NA

84 55 SYBR
Pgk1[1] Phosphoglycerate kinase 1
  • Live-trapped in the Brazilian coastal Atlantic Forest
  • Adult male or females without pregnant or lactating
GU361632

NA

191 55 SYBR
Actb[1] Beta-actin
  • Live-trapped in the Brazilian coastal Atlantic Forest
  • Adult male or females without pregnant or lactating
GU361629

NA

83 55 SYBR
Actg1[1] Gamma-actin 1, cytoplasmatic
  • Live-trapped in the Brazilian coastal Atlantic Forest
  • Adult male or females without pregnant or lactating
GU361630

NA

83 55 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Simone Sommer
  • Email: Sommer@izw-berlin.de
  • Institution: Leibniz Institute for Zoo and Wildlife Research (IZW), Evolutionary Genetics, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany

Citation Statistics

Cited by 13 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Weyrich A, Axtner J, Sommer S. Selection and validation of reference genes for real-time RT-PCR studies in the non-model species Delomys sublineatus, an endemic Brazilian rodent[J]. Biochemical and biophysical research communications, 2010, 392(2): 145-149.
  2. Weyrich A, Axtner J, Sommer S.Biochem Biophys Res Commun. 2010 Feb 5;392(2):145-9. doi: 10.1016/j.bbrc.2009.12.173. Epub 2010 Jan 6.PMID: 20059981

Categories