Difference between revisions of "Delomys sublineatus"

Revision as of 15:04, 23 June 2017

Pallid Atlantic Forest Rat (Delomys sublineatus), a rodent used as indicator species in conservation studies of the endangered Brazilian rainforest， suitable primer sets are more problematic to find than in model species. Out of nine tested primer sets designed for the fully sequenced Mus musculus, five could be used for the establishment of a proper running SYBR-Green assay and validation of their constant expression^[1] ^[2] .

Gene Symbol	Gene Name	Application Scope	Accession Number	Primers (5'-3') [Forward/Reverse]	Size [bp]	Tm [℃]	Detection
Sdha^[1]	Succinate dehydrogenase complex, subunit A	Universal reference genes	GU361627	NA	152	55	SYBR
Canx^[1]	Calnexin	Universal reference genes	GU361628	NA	84	55	SYBR
Pgk1^[1]	Phosphoglycerate kinase 1	Universal reference genes	GU361632	NA	191	55	SYBR
Actb^[1]	Beta-actin	Universal reference genes	GU361629	NA	83	55	SYBR
Actg1^[1]	Gamma-actin 1, cytoplasmatic	Universal reference genes	GU361630	NA	83	55	SYBR

Name: Simone Sommer
Email: Sommer@izw-berlin.de
Institution: Leibniz Institute for Zoo and Wildlife Research (IZW), Evolutionary Genetics, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany

Cited by 13 (Based on Google Scholar [2017-06-16])

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Weyrich A, Axtner J, Sommer S. Selection and validation of reference genes for real-time RT-PCR studies in the non-model species Delomys sublineatus, an endemic Brazilian rodent[J]. Biochemical and biophysical research communications, 2010, 392(2): 145-149.
↑ Weyrich A, Axtner J, Sommer S.Biochem Biophys Res Commun. 2010 Feb 5;392(2):145-9. doi: 10.1016/j.bbrc.2009.12.173. Epub 2010 Jan 6.PMID: 20059981

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-===Molecular Type===
+===Molecular Types===
 * mRNA