Delomys sublineatus

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Description

Delomys sublineatus-1.jpg
  • Pallid Atlantic Forest Rat (Delomys sublineatus), a rodent used as indicator species in conservation studies of the endangered Brazilian rainforest, suitable primer sets are more problematic to find than in model species. Out of nine tested primer sets designed for the fully sequenced Mus musculus, five could be used for the establishment of a proper running SYBR-Green assay and validation of their constant expression[1] [2] .

Liver Samples

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Sdha[1] Succinate dehydrogenase complex, subunit A
  • Universal reference genes
GU361627

NA

152 55 SYBR
Canx[1] Calnexin
  • Universal reference genes
GU361628

NA

84 55 SYBR
Pgk1[1] Phosphoglycerate kinase 1
  • Universal reference genes
GU361632

NA

191 55 SYBR
Actb[1] Beta-actin
  • Universal reference genes
GU361629

NA

83 55 SYBR
Actg1[1] Gamma-actin 1, cytoplasmatic
  • Universal reference genes
GU361630

NA

83 55 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Simone Sommer
  • Email: Sommer@izw-berlin.de
  • Institution: Leibniz Institute for Zoo and Wildlife Research (IZW), Evolutionary Genetics, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany

Citation Statistics

Cited by 13 (Based on Google Scholar [2017-06-16])

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Weyrich A, Axtner J, Sommer S. Selection and validation of reference genes for real-time RT-PCR studies in the non-model species Delomys sublineatus, an endemic Brazilian rodent[J]. Biochemical and biophysical research communications, 2010, 392(2): 145-149.
  2. Weyrich A, Axtner J, Sommer S.Biochem Biophys Res Commun. 2010 Feb 5;392(2):145-9. doi: 10.1016/j.bbrc.2009.12.173. Epub 2010 Jan 6.PMID: 20059981