Diabrotica virgifera virgifera

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Diabrotica virgifera virgifera-1.jpg
  • The western corn rootworm (WCR), Diabrotica v. virgifera, is one of the most important insect pests of cultivated maize in North America with annual losses in yield and control expenditures exceeding U.S. $1 billion annually. A number of strategies, such as chemical insecticides, crop rotation, biological control and transgenic plants expressing toxins from Bacillus thuringiensis (Bt), have been used to manage rootworm populations[1].
  • Common Name: Western corn rootworm
  • NCBI Taxonomy

Different Developmental Stages & Tissues

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
Size [bp] Tm [℃] Detection
β-actin[1] β-tubulin
  • Different life stages
133 60 SYBR
RPS9[1] ribosomal protein S9
  • dsRNA treatments
170 60 SYBR
EF1α[1] elongation factor-1α
  • Bt exposure and among different tissues
194 60 SYBR

Moleculer types

  • mRNA

Evaluation Methods


  • Name: Thaı ´s Barros Rodrigues
  • Email: thaisbarros.bio@gmail.com
  • Institute: CAPES Foundation, Ministry of Education of Brazil, Brası ´lia, Federal District, Brazil, and Federal University of Lavras, Lavras, Minas Gerais, Brazil

Citation Statistics

Cited by ?? (Based on Google Scholar [2017-08-01])


  1. 1.0 1.1 1.2 1.3 Rodrigues TB, Khajuria C, Wang H, Matz N, Cunha Cardoso D, Valicente FH, Zhou X, Siegfried B. Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). PLoS One. 2014 Oct 30;9(10):e109825. doi: 10.1371/journal.pone.0109825. eCollection 2014. Erratum in: PLoS One. 2015;10(4):e0124187. Barros Rodrigues, Thaís [corrected to Rodrigues, Thaís Barros]. PubMed PMID: 25356627; PubMed Central PMCID: PMC4214676.