Diabrotica virgifera virgifera
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Contents
Description
- The western corn rootworm (WCR), Diabrotica v. virgifera, is one of the most important insect pests of cultivated maize in North America with annual losses in yield and control expenditures exceeding U.S. $1 billion annually. A number of strategies, such as chemical insecticides, crop rotation, biological control and transgenic plants expressing toxins from Bacillus thuringiensis (Bt), have been used to manage rootworm populations [1].
- Common Name: Western corn rootworm
- NCBI Taxonomy
Different Developmental Stages & Tissues
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| β-actin[1] | actin 2 |
|
NM_001164951 |
|
133 | 60 | SYBR |
| RPS9[1] | ribosomal protein S9 |
|
XM_001608220 |
|
170 | 60 | SYBR |
| EF1α[1] | elongation factor-1α |
|
XM_003705302 |
|
194 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- ΔCt approach method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Thaı ´s Barros Rodrigues
- Email: thaisbarros.bio@gmail.com
- Institution: CAPES Foundation, Ministry of Education of Brazil, Brası ´lia, Federal District, Brazil, and Federal University of Lavras, Lavras, Minas Gerais, Brazil
Citation Statistics
Cited by 17 (Based on Google Scholar [2017-08-10])
References
- ↑ 1.0 1.1 1.2 1.3 Rodrigues TB, Khajuria C, Wang H, Matz N, Cunha Cardoso D, Valicente FH, Zhou X, Siegfried B. Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera). PLoS One. 2014 Oct 30;9(10):e109825. doi: 10.1371/journal.pone.0109825. eCollection 2014. Erratum in: PLoS One. 2015;10(4):e0124187. Barros Rodrigues, Thaís [corrected to Rodrigues, Thaís Barros]. PubMed PMID: 25356627; PubMed Central PMCID: PMC4214676.
