Difference between revisions of "Downloads"

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* Sequence Set of the experimental verified internal control genes ( in '''FASTA Format''') are provided for users to freely [ftp://download.big.ac.cn/bigd/GEN/ICG/ '''Download'''].   
 
* Sequence Set of the experimental verified internal control genes ( in '''FASTA Format''') are provided for users to freely [ftp://download.big.ac.cn/bigd/GEN/ICG/ '''Download'''].   
 
===Readme===
 
===Readme===
* Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources and complete CDS were extracted from the related peer-reviewed literature.
+
* Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources (e.g., NCBI accession number) and complete CDS were extracted from the related peer-reviewed literature.
 
* You can get access to the sequences directly with linux command ('''for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa''').
 
* You can get access to the sequences directly with linux command ('''for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa''').
 
* The candidate reference genes in your own genomes of transcriptomes could be selected through blast search against our provided sequences.
 
* The candidate reference genes in your own genomes of transcriptomes could be selected through blast search against our provided sequences.

Revision as of 08:47, 15 August 2017

ICG Sequence Download

  • Sequence Set of the experimental verified internal control genes ( in FASTA Format) are provided for users to freely Download.

Readme

  • Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources (e.g., NCBI accession number) and complete CDS were extracted from the related peer-reviewed literature.
  • You can get access to the sequences directly with linux command (for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa).
  • The candidate reference genes in your own genomes of transcriptomes could be selected through blast search against our provided sequences.
  • By the way, for primer design of potential identified internal control genes, an example of parameters for PRIMER 3.0 is as followings :
(a) Product size: 100-250 bp;
(b) Primer size: 19-25 bp;
(c) GC content : 45-55 %;
(d) Primer Tm : 56-62°C;