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ICG Sequence Download

• To promote the study on identification and characterization of potential internal control gene candidates from newly sequenced genomes or transcriptomes, sequence Set of the experimental verified internal control genes ( in FASTA Format) are provided for users to freely Download.

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• Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources (e.g., NCBI accession number) were obtained from the related peer-reviewed literature.
• You can get access to the sequences directly with linux command (for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa).
• The potential internal control gene candidates from the users' own data set are proposed to be identified through BLASTN search, against the curated sequences with customizable parameters, for example:

(a) Score value higher than 250;

(b) Identity value higher than 95%;

• By the way, for primer design of potential identified internal control genes, an example of parameters for PRIMER 3.0 is as followings :

(a) Product size: 100-250 bp;

(b) Primer size: 19-25 bp;

(c) GC content : 45-55 %;

(d) Primer Tm : 56-62°C;