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ICG Sequence Download
- Sequence Set of the experimental verified internal control genes ( in FASTA Format) are provided for users to freely Download.
- Considering the accuracy and completeness for your next-stage work, only the sequences with clear sources (e.g., NCBI accession number) and complete CDS were extracted from the related peer-reviewed literature.
- You can get access to the sequences directly with linux command (for example: wget ftp://download.big.ac.cn/bigd/GEN/ICG/ICG_V_1.fa).
- The candidate reference genes in your own genomes of transcriptomes could be selected through blast search against our provided sequences.
- By the way, for primer design of potential identified internal control genes, an example of parameters for PRIMER 3.0 is as followings :
- (a) Product size: 100-250 bp;
- (b) Primer size: 19-25 bp;
- (c) GC content : 45-55 %;
- (d) Primer Tm : 56-62°C;