Drosophila melanogaster
Contents
Description
- Drosophila melanogaster is a species of fly in the family Drosophilidae. It is known generally as the common fruit fly or vinegar fly and a model organism. It is widely used for biological research in studies of genetics, physiology, microbial pathogenesis, innate immunity and life history evolution. These advantages have enabled research in Drosophila important for fundamental biological processes and medical science[1].
- Common Name: Vinegar fly
- NCBI Taxonomy
Physiological Responses
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Actin[1] | Actin42A |
|
CG12051 |
|
138 | 60.08 | SYBR |
EF1[1] | Elongation Factor 1 alpha100 |
|
CG1873 |
|
125 | 60.94 | SYBR |
Mnf[1] | Forkhead domain 68A |
|
CG11799 |
|
66 | 59.83 | SYBR |
Rpl32[1] | Ribosomal protein L32 |
|
CG7939 |
|
107 | 59.82 | SYBR |
Rps20[1] | Ribosomal Protein S20 |
|
CG15693 |
|
87 | 60.97 | SYBR |
Tubulin[1] | Alpha-tubulin at 84B |
|
CG1913 |
|
96 | 60.9 | SYBR |
18S[1] | Small ribosomal submit |
|
CG8900 |
|
171 | 59.52 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Fleur Ponton
- Email: fleur_ponton@hotmail.com, fponton@sydney.edu.au
- Institution: School of Biological Sciences, Heydon-Laurence building - A08, The University of Sydney, NSW 2006, Australia
Citation Statistics
Cited by 144 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Ponton F, Chapuis M P, Pernice M, et al. Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster[J]. Journal of insect physiology, 2011, 57(6): 840-850.