Difference between revisions of "Elaeis guineensis"

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===Citation Statistics===
 
===Citation Statistics===
 
Cited by '''12''' (Based on Google Scholar [2017-06-16])
 
Cited by '''12''' (Based on Google Scholar [2017-06-16])
 +
 +
=='''Tissue Culture'''==
 +
 +
===Reference Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
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! Gene Symbol 
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! Gene Name
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! style="width=25%　font-size:9pt　"|Application Scope
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! Accession Number
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! Primer
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! Size [bp]
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! Tm [℃]
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! Detection
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|-
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|align="center"| PD00380<ref name="ref1"/>
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|align="center"| Predicted 40S ribosomal protein S27-2
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|
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*Tissue Culture
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|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EY397675 '''EY397675''']
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|nowrap style="font-size:9pt"|
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* F:GATGGTTCTTCCGAACGATATTGA
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* R:TCACATCCATGAAGAATGAGTTCG
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|align="center"| 113
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|align="center"| 63
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|align="center"| SYBR
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|-
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|align="center"| PD00569<ref name="ref1"/>
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|align="center"| Manganese superoxide dismutase
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|
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*Tissue Culture
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|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EL682210 '''EL682210''']
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|nowrap style="font-size:9pt"|
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* F:CACCACCAGACGTACATCACAAA
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* R:GATATGACCTCCGCCATTGAACT
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|align="center"| 129
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|}
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===Moleculer Type===
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* mRNA
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===Evaluation Methods===
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* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
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* [http://www.gene-quantification.com/bestkeeper.html '''BestKeeper method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15127793 '''Related Reference''']
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===Contact===
 +
*'''Name''': Pek-Lan Chan
 +
*'''Email''': chanpl@mpob.gov.my
 +
*'''Institution''': Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, Kajang, Selangor, Malaysia
  
 
=='''References'''==
 
=='''References'''==

Revision as of 09:19, 16 June 2017

Description

REFqPCR2014001-1.jpg
  • The African oil palm (Elaeis guineensis), which is grown in tropical and subtropical regions, is a highly productive oil-bearing crop.
  • The African oil palm (Elaeis guineensis), belonging to genus Elaeis in the family Arecaceae (Palmaceae), is the most productive oil- bearing crop grown in tropical and subtropical regions, accounting for 33% of vegetable oil and 45% of edible oil produced worldwide. Oil palm has a long productive life span and is frequently exposed to soil and atmospheric drought and other stresses.
  • Elaeis guineensis (E. guineensis) is the most productive oil variety that can produced 10–35 tonnes/ha of crude fruit bunch oil. It grows well in low land with humid places and tropical perennial plants which can cultivated easily in Malaysia. It is easy to get and readily available in the market. [1] [2].

Various Stresses

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
eIF1[1] Eukaryotic initiation factor 4A
  • Cold, drought and salinity treatments
 GAJH01031684.1
  • F:CCTCACCTATACTCTTCCCACCA
  • R:GTCATCGCCCAGGCACAG
 97 85.3 ± 0.18 SYBR
eIF2[1] Eukaryotic initiation factor 4A
  • Cold, drought and salinity treatments
 GAJH01015579.1
  • F:ATTAGGATGTTTGTGCTGGAT
  • R:CTCAGGAGGCATTGTGGC
 106 81.72 ± 0.16 SYBR
APT[1] Adenine phosphoribosyltransferase
  • Drought and salinity stress
 GAJH01036635.1
  • F:GTTTCAGGACATCACGACGCT
  • R:TAAGGCAATAGGGGGACCG
 102 82.34 ± 0.14 SYBR
cyc[1] Cyclophilin
  • Drought and salinity stress
 GAJH01023806.1
  • F:AGGGCTGTATGGGGATGATGT
  • R:GCTTTTACCTCCAGTTCCATTTC
 94 82.88 ± 0.14 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Wei Xia
  • Email: zjxiawei@gmail.com
  • Institution: Hainan Key Laboratory of Tropical Oil Crops Biology/Coconuts Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, PR China

Citation Statistics

Cited by 12 (Based on Google Scholar [2017-06-16])

Tissue Culture

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
PD00380[1] Predicted 40S ribosomal protein S27-2
  • Tissue Culture
 EY397675
  • F:GATGGTTCTTCCGAACGATATTGA
  • R:TCACATCCATGAAGAATGAGTTCG
 113 63 SYBR
PD00569[1] Manganese superoxide dismutase
  • Tissue Culture
 EL682210
  • F:CACCACCAGACGTACATCACAAA
  • R:GATATGACCTCCGCCATTGAACT
 129 60 SYBR

Moleculer Type

  • mRNA

Evaluation Methods

Contact

  • Name: Pek-Lan Chan
  • Email: chanpl@mpob.gov.my
  • Institution: Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, Kajang, Selangor, Malaysia

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Xia W, Mason AS, Xiao Y, et al. (2014) Analysis of multiple transcriptomes of the African oil palm (Elaeis guineensis) to identify reference genes for RT-qPCR. J Biotechnol 184, 63-73.
  2. ↑ Teo SH, Rashid U, Choong SYT, Taufiq-Yap YH (2017) Heterogeneous calcium-based bimetallic oxide catalyzed transesterification of Elaeis guineensis derived triglycerides for biodiesel production. Energy Conversion and Management 141, 20-27
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