Difference between revisions of "Festuca arundinacea"

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==Description==
 
==Description==
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[[File:REFqPCR2015022-1.jpg|right|327px|]]
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[[File:Festuca arundinacea.png|right|200px|link=Festuca arundinacea]]
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* Tall fescue (Festuca arundinacea Schreb.) is widely utilized as a major forage and turfgrass species in the temperate regions of the world and is a valuable plant material for studying molecular mechanismsof grass stress tolerance due to its superior drought and heat tolerance among cool-season species.
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* '''''Festuca arundinacea Schreb''''' is widely utilized as a major forage and turfgrass species in the temperate regions of the world. Tall fescue possesses superior drought and heat tolerance among cool-season perennial forage and turfgrass species and is typically used as model species to identify molecular mechanisms of stress tolerance in perennial grasses. As a long-lived perennial bunchgrass species, it grows vigorously and has prominent weed and disease tolerance<ref name="ref1"/><ref name="ref2"/>.
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* Tall fescue (Festuca arundinacea Schreb.) is the most widely-used cool-season species as forageand turfowing to its high qualityand productivity, aswellasa widerange ofstressadaptation. Tall fescue possesses superior drought and heat tolerance among cool-season perennial forage and turfgrass species and is typically used as model species to identify molecular mechanisms of stress tolerancein perennial grasses.
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* <font color=blue>'''Common Name:'''</font> '''Tall fescue'''
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* Tall fescue (Festuca arundinacea), belonging to the grass family Poaceae, subfamily Pooideae, and tribe Triticeae, is a hexaploid outcrossing species with a genome size of about 5.27–5.83 × 106 kilobase (kb). Tall fescue is a major cool-season grass that is widely distributed in temperate regions throughout the world. As a long-lived perennial bunchgrass species, it grows vigorously and has prominent weed and disease tolerance.  <ref name="ref1"/> <ref name="ref2"/>.
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=4606 <font color=blue>'''NCBI Taxonomy'''</font>]
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=='''''Abiotic Stresses'''''==
 
=='''''Abiotic Stresses'''''==
  
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===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
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! style="width=25%　font-size:9pt　"|Application Scope  
 
! style="width=25%　font-size:9pt　"|Application Scope  
 
! Accession Number  
 
! Accession Number  
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! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|-
 
|-
 
|align="center"| GAPDH<ref name="ref1"/>
 
|align="center"| GAPDH<ref name="ref1"/>
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|align="center"| glyceraldehyde 3-phosphate dehydrogenase
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|align="center"| Glyceraldehyde 3-phosphate dehydrogenase
 
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|
 
* Roots and leaves under drought stress  
 
* Roots and leaves under drought stress  
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:TGAGAAGGCAGCCACCTATG
 
* F:TGAGAAGGCAGCCACCTATG
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* R:TGAGAAGGCAGCCACCTATG
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* R:TGCTGTCACCCTGGAAGTCA
 
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GAACCAAGACACTATGCAAACA
 
* F:GAACCAAGACACTATGCAAACA
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* R:GAACCAAGACACTATGCAAACA
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* R:GAAATACCACTATCCGCTAACTCA
 
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:ATGCTTTCGTCTTATGCCC
 
* F:ATGCTTTCGTCTTATGCCC
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* R:ATGCTTTCGTCTTATGCCC
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* R:CTCTTGGTTTTGATGGTTGC
 
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GAACATCCTCCTTGTCAGCA
 
* F:GAACATCCTCCTTGTCAGCA
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* R:GAACATCCTCCTTGTCAGCA
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* R:CCTCATTGTAACCGCCAGA
 
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GCCAAATGTCTGGTGCTTAG
 
* F:GCCAAATGTCTGGTGCTTAG
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* R:GCCAAATGTCTGGTGCTTAG
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* R:TCATCCGCTTCGTCTTCAA
 
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|-
 
|-
 
|align="center"| CACS<ref name="ref1"/>
 
|align="center"| CACS<ref name="ref1"/>
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|align="center"| clathrin adaptor complex subunit
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|align="center"| Clathrin adaptor complex subunit
 
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* Heat-stressed roots  
 
* Heat-stressed roots  
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:TCGCTACATCACGAGGGCT
 
* F:TCGCTACATCACGAGGGCT
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* R:TCGCTACATCACGAGGGCT
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* R:AACAGGATACGGGGGAAGAATA
 
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|nowrap style="font-size:9pt"|
 
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* F:AGATCAAGGTCGTTGCTCCA
 
* F:AGATCAAGGTCGTTGCTCCA
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* R:AGATCAAGGTCGTTGCTCCA
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* R:CTCCCAGACTAGACGATACAGC
 
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|nowrap style="font-size:9pt"|
 
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* F:ACCCAAGATTTCGAGCTGTAT
 
* F:ACCCAAGATTTCGAGCTGTAT
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* R:ACCCAAGATTTCGAGCTGTAT
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* R:AACCTAAACCTCACATATCTCCC
 
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|}
 
|}
  
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===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
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===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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===Citation Statistics===
 
===Citation Statistics===
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Cited by '''10''' (Based on Google Scholar [2017-06-16])
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Cited by [https://scholar.google.com/scholar?cites=15511454582359549543&as_sdt=2005&sciodt=0,5&hl=en'''12'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
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[[Category:Plants]]
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=='''Categories'''==
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[[Category:mRNA]]　
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[[Category:Plants]][[Category:mRNA]][[Category:SYBR]][[Category:ACT]] [[Category:CACS]] [[Category:F-box]] [[Category:GAPDH]] [[Category:PEPKR]] [[Category:SAND]] [[Category:TIP41]] [[Category:Tubulin]][[Category:Abiotic Stress]]
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[[Category:SYBR]]　
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[[Category:geNorm]][[Category:NormFinder]]
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[[Category:BestKeeper]]
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[[Category:Delta Ct]]
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[[Category:RefFinder]]

Latest revision as of 06:43, 1 September 2017

Description

Festuca arundinacea.png
  • Festuca arundinacea Schreb is widely utilized as a major forage and turfgrass species in the temperate regions of the world. Tall fescue possesses superior drought and heat tolerance among cool-season perennial forage and turfgrass species and is typically used as model species to identify molecular mechanisms of stress tolerance in perennial grasses. As a long-lived perennial bunchgrass species, it grows vigorously and has prominent weed and disease tolerance[1][2].
  • Common Name: Tall fescue
  • NCBI Taxonomy

Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
GAPDH[1] Glyceraldehyde 3-phosphate dehydrogenase
  • Roots and leaves under drought stress
 GT035008
  • F:TGAGAAGGCAGCCACCTATG
  • R:TGCTGTCACCCTGGAAGTCA
 125 58 SYBR
TIP41[1] TIP41-like family protein
  • Roots and leaves under drought stress
  • Salt-treated roots or leaves
  • Cold-treated roots & leaves
  • Heat-stressed leaves
 DT690696
  • F:GAACCAAGACACTATGCAAACA
  • R:GAAATACCACTATCCGCTAACTCA
 162 58 SYBR
TUB[1] Alpha Tubulin
  • Roots and leaves under drought stress
  • Salt-treated roots or leaves
  • Cold-treated roots & leaves
  • Heat-stressed leaves
 GT051159
  • F:ATGCTTTCGTCTTATGCCC
  • R:CTCTTGGTTTTGATGGTTGC
 215 58 SYBR
PEPKR1[1] Phosphoenolpyruvate carboxylase-related Kinase 1
  • Cold-treated roots
 DT688788
  • F:GAACATCCTCCTTGTCAGCA
  • R:CCTCATTGTAACCGCCAGA
 155 58 SYBR
F-box[1] F-box/kelch-repeat protein
  • Cold-treated leaves
 GT039249
  • F:GCCAAATGTCTGGTGCTTAG
  • R:TCATCCGCTTCGTCTTCAA
 101 58 SYBR
CACS[1] Clathrin adaptor complex subunit
  • Heat-stressed roots
 GT044151
  • F:TCGCTACATCACGAGGGCT
  • R:AACAGGATACGGGGGAAGAATA
 255 58 SYBR
ACT[1] Actin7
  • Heat-stressed leaves
 GT038376
  • F:AGATCAAGGTCGTTGCTCCA
  • R:CTCCCAGACTAGACGATACAGC
 189 58 SYBR
SAND[1] SAND family protein
  • Salt-treated roots or leaves
 GT037941
  • F:ACCCAAGATTTCGAGCTGTAT
  • R:AACCTAAACCTCACATATCTCCC
 188 58 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Zhimin Yang
  • Email: nauyzm@njau.edu.cn
  • Institution: College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu, China

Citation Statistics

Cited by 12 (Based on Google Scholar [2017-09-01])

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Yang Z, Chen Y, Hu B, Tan Z, Huang B (2015) Identification and validation of reference genes for quantification of target gene expression with quantitative real-time PCR for tall fescue under four abiotic stresses. PLoS One 10, e0119569.
  2. ↑ Li H, Hu T, Amombo E, Fu J (2017) Genome-wide identification of heat stress-responsive small RNAs in tall fescue (Festuca arundinacea) by high-throughput sequencing. J Plant Physiol 213, 157-165.

Categories

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