Difference between revisions of "Gallus gallus"
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=='''''Lymphoid Organs'''''== | =='''''Lymphoid Organs'''''== | ||
| − | === | + | ===Internal Control Genes=== |
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===Citation Statistics=== | ===Citation Statistics=== | ||
| − | Cited by ''' | + | Cited by [https://scholar.google.com/scholar?cites=2315072174273555406&as_sdt=2005&sciodt=0,5&hl=en '''6'''] (Based on Google Scholar [2017-09-01]) |
=='''''Embryo Fibroblasts'''''== | =='''''Embryo Fibroblasts'''''== | ||
| − | === | + | ===Internal Control Genes=== |
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*'''Institution''': Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China | *'''Institution''': Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China | ||
===Citation Statistics=== | ===Citation Statistics=== | ||
| − | Cited by ''' | + | Cited by [https://scholar.google.com/scholar?cites=13405743498904358751&as_sdt=2005&sciodt=0,5&hl=en '''25'''] (Based on Google Scholar [2017-09-01]) |
=='''References'''== | =='''References'''== | ||
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</ref> | </ref> | ||
</references> | </references> | ||
| − | + | =='''Categories'''== | |
[[Category:Animals]] | [[Category:Animals]] | ||
| − | [[Category:mRNA]] | + | [[Category:mRNA]] |
[[Category:SYBR]] | [[Category:SYBR]] | ||
[[Category:Other rRNA]] [[Category:ACT]] [[Category:GAPDH]] [[Category:HMBS]] [[Category:HPRT]] [[Category:TBP]] | [[Category:Other rRNA]] [[Category:ACT]] [[Category:GAPDH]] [[Category:HMBS]] [[Category:HPRT]] [[Category:TBP]] | ||
Latest revision as of 08:02, 1 September 2017
Contents
Description
- Gallus gallus is a tropical member of the family Phasianidae, it is the primary progenitor of the domestic chicken. The red junglefowl was first domesticated at least five thousand years ago in Asia. Since then it has spread around the world, and the domestic form is kept globally as a productive food source of both meat and eggs. Gallus gallus is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and serves as the main laboratory model for the approximately 9,600 extant avian species [1] [2].
- Common Name: Red junglefowl
- NCBI Taxonomy
Lymphoid Organs
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| TBP[1] | TATA binding protein |
|
AF221563 |
|
134 | 60 | SYBR |
| GAPDH[1] | Glyceraldehyde-3-phosphate dehydrogenase |
|
AF047874 |
|
150 | 60 | SYBR |
| r28S[1] | 28S rRNA gene |
|
FM165415 |
|
62 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: D. Borowska
- Email: dominika.borowska@roslin.ed.ac.uk
- Institution: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom
Citation Statistics
Cited by 6 (Based on Google Scholar [2017-09-01])
Embryo Fibroblasts
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| ACTB[2] | Beta-actin |
|
L08165 |
|
183 | NA | SYBR |
| HPRT1[2] | Hypoxanthine phosphorribosyltransferase 1 |
|
NM_204848 |
|
177 | NA | SYBR |
| HMBS[2] | Hydroxymethylbilane synthase |
|
XM_417846 |
|
131 | NA | SYBR |
Molecular types
- mRNA
Evaluation Methods
- ΔCt approach method && Related Reference
Contact
- Name: Zhuang Ding
- Email: renfu.yin@helmholtz-muenchen.de
- Institution: Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China
Citation Statistics
Cited by 25 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3 Borowska D, Rothwell L, Bailey RA, Watson K, Kaiser P (2016) Identification of stable reference genes for quantitative PCR in cells derived from chicken lymphoid organs. Vet Immunol Immunopathol 170, 20-24.
- ↑ 2.0 2.1 2.2 2.3 Yin R, Liu X, Liu C, et al. Systematic selection of housekeeping genes for gene expression normalization in chicken embryo fibroblasts infected with Newcastle disease virus[J]. Biochemical and biophysical research communications, 2011, 413(4): 537-540.
