Difference between revisions of "Gallus gallus"

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==Description==
 
==Description==
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[[File:REFqPCR2016011-1.jpg|right|227px|]]
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[[File:Gallus gallus.png|right|200px|link=Gallus gallus]]
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* "Gallus gallus" redirects here. For other subspecies, see Chicken. The red junglefowl (Gallus gallus) is a tropical member of the family Phasianidae. It is the primary progenitor of the domestic chicken (though genetic evidence strongly suggests some past hybridisation with the grey junglefowl as well). The red junglefowl was first domesticated at least five thousand years ago in Asia. Since then it has spread around the world, and the domestic form is kept globally as a very productive food source of both meat and eggs.
+
* '''''Gallus gallus''''' is a tropical member of the family Phasianidae, it is the primary progenitor of the domestic chicken. The red junglefowl was first domesticated at least five thousand years ago in Asia. Since then it has spread around the world, and the domestic form is kept globally as a productive food source of both meat and eggs. ''Gallus gallus'' is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and serves as the main laboratory model for the approximately 9,600 extant avian species <ref name="ref1"/> <ref name="ref2"/>.
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* The chicken (Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and serves as the main laboratory model for the approx9,600 extant avian species. The chicken also represents the first agricultural animal to have its genome sequenced. Modern birds (Ornithurae) evolved from therapod dinosaurs9, 10 in the middle of the Mesozoic era.  <ref name="ref1"/> <ref name="ref2"/>.
+
* <font color=blue>'''Common Name:'''</font> '''Red junglefowl'''
 +
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9031 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Lymphoid Organs'''''==
 
=='''''Lymphoid Organs'''''==
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===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25%　font-size:9pt　"|Application Scope  
 
! style="width=25%　font-size:9pt　"|Application Scope  
 
! Accession Number  
 
! Accession Number  
−
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|-
 
|-
 
|align="center"| TBP<ref name="ref1"/>
 
|align="center"| TBP<ref name="ref1"/>
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|align="center"| TA-TA binding protein 1 mRNA, partial cds
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|align="center"| TATA binding protein  
 
|
 
|
 
* Chicken lymphoid organs  
 
* Chicken lymphoid organs  
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:AGCTCTGGGATAGTGCCACAG
 
* F:AGCTCTGGGATAGTGCCACAG
−
* R:AGCTCTGGGATAGTGCCACAG
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* R:ATAATAACAGCAGCAAAACGCTTG
 
|align="center"|  134  
 
|align="center"|  134  
 
|align="center"| 60
 
|align="center"| 60
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|-
 
|-
 
|align="center"| GAPDH<ref name="ref1"/>
 
|align="center"| GAPDH<ref name="ref1"/>
−
|align="center"| glyceraldehyde-3-phosphate dehydrogenase mRNA,complete cds.
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|align="center"| Glyceraldehyde-3-phosphate dehydrogenase
 
|
 
|
 
* Chicken lymphoid organs  
 
* Chicken lymphoid organs  
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GAAGGCTGGGGCTCATCTG
 
* F:GAAGGCTGGGGCTCATCTG
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* R:GAAGGCTGGGGCTCATCTG
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* R:CAGTTGGTGGTGCACGATG
 
|align="center"|  150  
 
|align="center"|  150  
 
|align="center"| 60
 
|align="center"| 60
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|-
 
|-
 
|align="center"| r28S<ref name="ref1"/>
 
|align="center"| r28S<ref name="ref1"/>
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|align="center"| 28S rRNA gene, clone GgLSU-1.
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|align="center"| 28S rRNA gene
 
|
 
|
 
* Chicken lymphoid organs  
 
* Chicken lymphoid organs  
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|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GGCGAAGCCAGAGGAAACT
 
* F:GGCGAAGCCAGAGGAAACT
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* R:GGCGAAGCCAGAGGAAACT
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* R:GACGACCGATTTGCACGTC
 
|align="center"|  62  
 
|align="center"|  62  
 
|align="center"| 60
 
|align="center"| 60
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|}
 
|}
  
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===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
 
===Evaluation Methods===  
 
===Evaluation Methods===  
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*'''Institution''': The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom
 
*'''Institution''': The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom
  
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==Citation Statistics==
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===Citation Statistics===
−
Cited by '''5''' (Based on Google Scholar [2017-06-01])
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Cited by [https://scholar.google.com/scholar?cites=2315072174273555406&as_sdt=2005&sciodt=0,5&hl=en '''6'''] (Based on Google Scholar [2017-09-01])
  
 
=='''''Embryo Fibroblasts'''''==
 
=='''''Embryo Fibroblasts'''''==
−
===Reference Genes===
+
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25%　font-size:9pt　"|Application Scope  
 
! style="width=25%　font-size:9pt　"|Application Scope  
 
! Accession Number  
 
! Accession Number  
−
! Primer
+
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
 
! Detection
 
! Detection
 
|-
 
|-
−
|align="center"|  ACTB<ref name="ref1"/>
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|align="center"|  ACTB<ref name="ref2"/>
 
|align="center"| Beta-actin  
 
|align="center"| Beta-actin  
 
|
 
|
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in chicken embryo fibroblast following NDV treatment over a time up to 72 h
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*In chicken embryo fibroblast following NDV treatment over a time up to 72 h
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/L08165.1 '''L08165''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/L08165.1 '''L08165''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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* F:cagacatcagggtgtgatgg
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* F:CAGACATCAGGGTGTGATGG
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* R: tcaggggctactctcagctc
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* R: TCAGGGGCTACTCTCAGCTC
 
|align="center"| 183
 
|align="center"| 183
 
|align="center"| NA
 
|align="center"| NA
 
|align="center"| SYBR
 
|align="center"| SYBR
 
|-
 
|-
−
|align="center"| HPRT1<ref name="ref1"/>
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|align="center"| HPRT1<ref name="ref2"/>
 
|align="center"| Hypoxanthine phosphorribosyltransferase 1  
 
|align="center"| Hypoxanthine phosphorribosyltransferase 1  
 
|
 
|
−
in chicken embryo fibroblast following NDV treatment over a time up to 73 h
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*In chicken embryo fibroblast following NDV treatment over a time up to 73 h
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_204848.1 '''NM_204848''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_204848.1 '''NM_204848''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
−
* F: tggtggggatgacctctcaa
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* F: TGGTGGGGATGACCTCTCAA
−
* R: ggccgatatcccacacttcg
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* R: GGCCGATATCCCACACTTCG
 
|align="center"| 177
 
|align="center"| 177
 
|align="center"| NA
 
|align="center"| NA
 
|align="center"| SYBR
 
|align="center"| SYBR
 
|-
 
|-
−
|align="center"|  HMBS<ref name="ref1"/>
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|align="center"|  HMBS<ref name="ref2"/>
 
|align="center"| Hydroxymethylbilane synthase  
 
|align="center"| Hydroxymethylbilane synthase  
 
|
 
|
−
in chicken embryo fibroblast following NDV treatment over a time up to 74 h
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*In chicken embryo fibroblast following NDV treatment over a time up to 74 h
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_417846 '''XM_417846''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_417846 '''XM_417846''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
−
* F: ggctgggagaatcgcatagg
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* F: GGCTGGGAGAATCGCATAGG
−
* R: ggccgatatcccacacttcg
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* R: GGCCGATATCCCACACTTCG
 
|align="center"| 131
 
|align="center"| 131
 
|align="center"| NA
 
|align="center"| NA
 
|align="center"| SYBR
 
|align="center"| SYBR
 
|}
 
|}
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===Moleculer types===
+
 
 +
===Molecular types===
 
* mRNA
 
* mRNA
 
===Evaluation Methods===
 
===Evaluation Methods===
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*'''Name''':  Zhuang Ding
 
*'''Name''':  Zhuang Ding
 
*'''Email''': renfu.yin@helmholtz-muenchen.de
 
*'''Email''': renfu.yin@helmholtz-muenchen.de
−
*'''Institute''': Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China
+
*'''Institution''': Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=13405743498904358751&as_sdt=2005&sciodt=0,5&hl=en '''25'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
<ref name="ref2">
 
<ref name="ref2">
−
Hillier LW, Miller W, Birney E, et al. (2004) Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432, 695-716.
+
Yin R, Liu X, Liu C, et al. Systematic selection of housekeeping genes for gene expression normalization in chicken embryo fibroblasts infected with Newcastle disease virus[J]. Biochemical and biophysical research communications, 2011, 413(4): 537-540.
 
</ref>
 
</ref>
 
</references>
 
</references>
 +
=='''Categories'''==
 +
[[Category:Animals]]
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[[Category:mRNA]]
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[[Category:SYBR]]
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[[Category:Other rRNA]] [[Category:ACT]] [[Category:GAPDH]] [[Category:HMBS]] [[Category:HPRT]] [[Category:TBP]]
 +
[[Category:Specific Tissue]]  [[Category:Different Developmental Stages]]
  
−
[[Category:Animals]]
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[[Category:geNorm]]
 +
[[Category:NormFinder]]
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[[Category:BestKeeper]]
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[[Category:Delta Ct]]

Latest revision as of 08:02, 1 September 2017

Description

Gallus gallus.png
  • Gallus gallus is a tropical member of the family Phasianidae, it is the primary progenitor of the domestic chicken. The red junglefowl was first domesticated at least five thousand years ago in Asia. Since then it has spread around the world, and the domestic form is kept globally as a productive food source of both meat and eggs. Gallus gallus is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and serves as the main laboratory model for the approximately 9,600 extant avian species [1] [2].
  • Common Name: Red junglefowl
  • NCBI Taxonomy

Lymphoid Organs

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
TBP[1] TATA binding protein
  • Chicken lymphoid organs
 AF221563
  • F:AGCTCTGGGATAGTGCCACAG
  • R:ATAATAACAGCAGCAAAACGCTTG
 134 60 SYBR
GAPDH[1] Glyceraldehyde-3-phosphate dehydrogenase
  • Chicken lymphoid organs
 AF047874
  • F:GAAGGCTGGGGCTCATCTG
  • R:CAGTTGGTGGTGCACGATG
 150 60 SYBR
r28S[1] 28S rRNA gene
  • Chicken lymphoid organs
 FM165415
  • F:GGCGAAGCCAGAGGAAACT
  • R:GACGACCGATTTGCACGTC
 62 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: D. Borowska
  • Email: dominika.borowska@roslin.ed.ac.uk
  • Institution: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom

Citation Statistics

Cited by 6 (Based on Google Scholar [2017-09-01])

Embryo Fibroblasts

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
ACTB[2] Beta-actin
  • In chicken embryo fibroblast following NDV treatment over a time up to 72 h
 L08165
  • F:CAGACATCAGGGTGTGATGG
  • R: TCAGGGGCTACTCTCAGCTC
 183 NA SYBR
HPRT1[2] Hypoxanthine phosphorribosyltransferase 1
  • In chicken embryo fibroblast following NDV treatment over a time up to 73 h
 NM_204848
  • F: TGGTGGGGATGACCTCTCAA
  • R: GGCCGATATCCCACACTTCG
 177 NA SYBR
HMBS[2] Hydroxymethylbilane synthase
  • In chicken embryo fibroblast following NDV treatment over a time up to 74 h
 XM_417846
  • F: GGCTGGGAGAATCGCATAGG
  • R: GGCCGATATCCCACACTTCG
 131 NA SYBR

Molecular types

  • mRNA

Evaluation Methods

Contact

  • Name: Zhuang Ding
  • Email: renfu.yin@helmholtz-muenchen.de
  • Institution: Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China

Citation Statistics

Cited by 25 (Based on Google Scholar [2017-09-01])

References

  1. ↑ 1.0 1.1 1.2 1.3 Borowska D, Rothwell L, Bailey RA, Watson K, Kaiser P (2016) Identification of stable reference genes for quantitative PCR in cells derived from chicken lymphoid organs. Vet Immunol Immunopathol 170, 20-24.
  2. ↑ 2.0 2.1 2.2 2.3 Yin R, Liu X, Liu C, et al. Systematic selection of housekeeping genes for gene expression normalization in chicken embryo fibroblasts infected with Newcastle disease virus[J]. Biochemical and biophysical research communications, 2011, 413(4): 537-540.

Categories

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