Gallus gallus

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Description

REFqPCR2016011-1.jpg
  • "Gallus gallus" redirects here. For other subspecies, see Chicken. The red junglefowl (Gallus gallus) is a tropical member of the family Phasianidae. It is the primary progenitor of the domestic chicken (though genetic evidence strongly suggests some past hybridisation with the grey junglefowl as well). The red junglefowl was first domesticated at least five thousand years ago in Asia. Since then it has spread around the world, and the domestic form is kept globally as a very productive food source of both meat and eggs.
  • The chicken (Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates and serves as the main laboratory model for the approx9,600 extant avian species. The chicken also represents the first agricultural animal to have its genome sequenced. Modern birds (Ornithurae) evolved from therapod dinosaurs9, 10 in the middle of the Mesozoic era. [1] [2].

Lymphoid Organs

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
TBP[1] TA-TA binding protein 1 mRNA, partial cds
  • Chicken lymphoid organs
AF221563
  • F:AGCTCTGGGATAGTGCCACAG
  • R:AGCTCTGGGATAGTGCCACAG
134 60 SYBR
GAPDH[1] glyceraldehyde-3-phosphate dehydrogenase mRNA,complete cds.
  • Chicken lymphoid organs
AF047874
  • F:GAAGGCTGGGGCTCATCTG
  • R:GAAGGCTGGGGCTCATCTG
150 60 SYBR
r28S[1] 28S rRNA gene, clone GgLSU-1.
  • Chicken lymphoid organs
FM165415
  • F:GGCGAAGCCAGAGGAAACT
  • R:GGCGAAGCCAGAGGAAACT
62 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: D. Borowska
  • Email: dominika.borowska@roslin.ed.ac.uk
  • Institution: The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom

Citation Statistics

Cited by 5 (Based on Google Scholar [2017-06-01])

Embryo Fibroblasts

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ACTB[2] Beta-actin

in chicken embryo fibroblast following NDV treatment over a time up to 72 h

L08165
  • F:cagacatcagggtgtgatgg
  • R: tcaggggctactctcagctc
183 NA SYBR
HPRT1[2] Hypoxanthine phosphorribosyltransferase 1

in chicken embryo fibroblast following NDV treatment over a time up to 73 h

NM_204848
  • F: tggtggggatgacctctcaa
  • R: ggccgatatcccacacttcg
177 NA SYBR
HMBS[2] Hydroxymethylbilane synthase

in chicken embryo fibroblast following NDV treatment over a time up to 74 h

XM_417846
  • F: ggctgggagaatcgcatagg
  • R: ggccgatatcccacacttcg
131 NA SYBR

Moleculer types

  • mRNA

Evaluation Methods

Contact

  • Name: Zhuang Ding
  • Email: renfu.yin@helmholtz-muenchen.de
  • Institute: Department of Veterinary Preventive Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Xi’an Road 5333, Changchun, Jilin 130062, China

Citation Statistics

Cited by 5 (Based on Google Scholar [2017-06-01])

References

  1. 1.0 1.1 1.2 1.3 Borowska D, Rothwell L, Bailey RA, Watson K, Kaiser P (2016) Identification of stable reference genes for quantitative PCR in cells derived from chicken lymphoid organs. Vet Immunol Immunopathol 170, 20-24.
  2. 2.0 2.1 2.2 2.3 Yin R, Liu X, Liu C, et al. Systematic selection of housekeeping genes for gene expression normalization in chicken embryo fibroblasts infected with Newcastle disease virus[J]. Biochemical and biophysical research communications, 2011, 413(4): 537-540.