Gene:EF1A

Experimental Validated ICGs

Species	Gene Synonymous	Application scenarios	Publication	Year
Agrostis stolonifera	Elongation factor-1a	Abiotic Stresses	Selection of reference genes for quantitative real-time PCR normalization in creeping bentgrass involved in four abiotic stresses	2015
Ammopiptanthus mongolicus	Elongation factor 1-alpha	Abiotic Stresses	Reference gene selection for qPCR in Ammopiptanthus mongolicus under abiotic stresses and expression analysis of seven ROS-scavenging enzyme genes	2012
Anastrepha obliqua	Elongation factor-1 alpha	Different Developmental Stages	Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua	2016
Aphis gossypii	Elongation factor 1 alpha	Different Developmental Stages & Geographical Populations	Identification and Validation of Reference Genes for the Normalization of Gene Expression Data in qRT-PCR Analysis in Aphis gossypii (Hemiptera: Aphididae).	2016
Arachis hypogaea	Elongation factor 1B	Different Tissues	Evaluation and validation of reference genes for normalization of quantitative real-time PCR based gene expression studies in peanut	2013
Arachis hypogaea	Elongation factor 1b	Different Developmental Stages & Abiotic Stress	Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR	2012
Artemisia annua	Elongation factor 1-alpha	Different Tissues & Abiotic Stress	Reference gene selection in Artemisia annua L., a plant species producing anti-malarial artemisinin	2014
Bemisia tabaci	Elongation factor1-alpha	Different Commercially Available Insecticides	Expression profiling in Bemisia tabaci under insecticide treatment: indicating the necessity for custom reference gene selection	2014
Brachiaria brizantha	Eukaryotic initiation factor 4A	Different Sex & Tissues	Validation of internal reference genes for real-time quantitative polymerase chain reaction studies in the tick, Ixodes scapularis (Acari: Ixodidae)	2013
Branchiostoma japonicum	Elongation factor 1-alpha	Elongation factor 1-alpha	EF1a is a useful internal reference for studies of gene expression regulation in amphioxus Branchiostoma japonicum	2012
Brassica oleracea	Elongation factor 1-alpha	Abiotic Stresses	Identification of suitable qPCR reference genes in leaves of Brassica oleracea under abiotic stresses	2014
Brassica rapa subsp. pekinensis	Elongation-factor-1-α	Biotic Stress & Mycotic Infection	Reference gene selection for real-time quantitative polymerase chain reaction of mRNA transcript levels in Chinese cabbage (Brassica rapa L. ssp. pekinensis)	2010
Camellia sinensis	Elongation factor 1 alpha	Various Experimental Treatments	Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze)	2014
Caragana korshinskii	Elongation factor 1-alpha-like	Abiotic Stress	Reference gene selection for qRT-PCR in Caragana korshinskii Kom. under different stress conditions	2014
Chortoicetes terminifera	Elongation factor 1 alpha	Density-Dependent Behavioural plasticity	Assessment and validation of a suite of reverse transcription-quantitative PCR reference genes for analyses of density-dependent behavioural plasticity in the Australian plague locust	2011
Cicer arietinum	Elongation factor 1-alpha	Different Developmental Stages & Abiotic Stress	Validation of internal control genes for quantitative gene expression studies in chickpea (Cicer arietinum L.). Biochemical and biophysical research communications	2010
Cichorium intybus	Elongation factor 1-alpha	Different Tissues	Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR	2010
Coffea arabica L.	Elongation factor 1, Elongation factor 1-alpha	Abiotic Stress	Nitrogen starvation, salt and heat stress in coffee (Coffea arabica L.): identification and validation of new genes for qPCR normalization	2013
Coffea arabica L.	Elongation factor 1-alpha	Different Genotypes & Abiotic Stress	Selection of Reference Genes for Normalizing Quantitative Real-Time PCR Gene Expression Data with Multiple Variables in Coffea spp	2012
Corchorus capsularis	Elongation factor 1-alph	Different Tissues & Abiotic Stress	Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments	2015
Craterostigma plantagineum	Eukaryotic initiation factor 5A, Elongation factor 1-alpha	Different Tissues	Quantification of expression of dehydrin isoforms in the desiccation tolerant plant Craterostigma plantagineum using specifically designed reference genes	2015
Ctenopharyngodon idella	Elongation factor 1 a	Tissues & Cell Culture	Evaluation of internal control genes for qRT-PCR normalization in tissues and cell culture for antiviral studies of grass carp (Ctenopharyngodon idella)	2011
Cucumis sativus	Elongation factor 1-alpha	Various Nutrition Conditions	Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition	2013
Cucumis sativus	Elongation factor 1-α	Different Tissues & Hormones Treatment & abiotic stresses	Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber	2010
Cynodon dactylon	Elongation factor 1a	Abiotic Stress	Selection and validation of reference genes for target gene analysis with quantitative RT-PCR in leaves and roots of bermudagrass under four different abiotic stresses	2014
Danaus plexippus	Elongation factor 1α	Biotic & Abiotic Stress	Selection of Reference Genes for RT-qPCR Analysis in the Monarch Butterfly, Danaus plexippus (L.), a Migrating Bio-Indicator	2015
Dendrocalamus latiflorus	Elongation factor 1-alpha	Different Tissues & Ploidy	Validation of Reference Genes Aiming Accurate Normalization of qRT-PCR Data in Dendrocalamus latiflorus Munro	2014
Diabrotica virgifera virgifera	elongation factor-1α	Different Developmental Stages & Tissues	Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera)	2014
Dicentrarchus labrax	Ef-1 alpha	Different Developmental Stages & Tissues	Evaluation of candidate reference genes for QPCR during ontogenesis and of immune-relevant tissues of European seabass (Dicentrarchus labrax)	2009
Dimocarpus longan	Eukaryotic elongation factor 1-alpha	Somatic Embryogenesis	Reference gene selection for qPCR analysis during somatic embryogenesis in longan tree	2010
Diploptera punctata	Elongation factor 1 alpha	During Gonadotrophic Cycle	Sequencing and validation of housekeeping genes for quantitative real-time PCR during the gonadotrophic cycle of Diploptera punctata	2013
Drosophila melanogaster	Elongation Factor 1 alpha100	Physiological Responses	Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster	2011
Eremosparton songoricum	Elongation factor 1-alpha	Different Tissues & Abiotic Stress	Reference gene selection in the desert plant Eremosparton songoricum	2012
Eucalyptus globulus	Elongation factor 1α	Cold Acclimation	Validation of reference genes for real-time qRT-PCR normalization during cold acclimation in Eucalyptus globulus	2010
Fragaria ananassa	Elongation factor 1-alpha	Different Developmental Stages & Tissues	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses	2015
Gentiana macrophylla	Elongation factor 1-alpha	Different Tissue Types & Abiotic Stress	Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla	2016
Gossypium hirsutum	Translation elongation factor 1A-8	Abiotic Stress	Evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (Gossypium hirsutum L.)	2013
Haliotis discus hannai	Elongation factor-1-а	Bacterial Infection	Identification of normalization factors for quantitative real-time RT-PCR analysis of gene expression in Pacific abalone Haliotis discus hannai	2013
Hippoglossus hippoglossus	Elongation factor 1 alpha	Different Development & Tissues	Evaluation of potential reference genes for real time RT-PCR studies in Atlantic halibut (Hippoglossus Hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes	2010
Homo sapiens	Eukaryotic elongation factor 1A1	Myocardium	Genomic selection of reference genes for real-time PCR in human myocardium	2008
Hyriopsis cumingii	Elongation factor 1-alpha	During Biomineralization	Identification of housekeeping genes suitable for gene expression analysis in the pearl mussel, Hyriopsis cumingii, during biomineralization	2014
Jatropha curcas	Elongation factor 1-alpha	Staminate & Pistillate Flowers	Identification and validation of superior reference gene for gene expression normalization via RT-qPCR in staminate and pistillate flowers of Jatropha curcas - A biodiesel plant.	2017
Leptospira interrogans	Eukaryotic translation elongation factor 1 alpha 2	Different Tissues & Abiotic Stress	Selection of the internal control gene for real-time quantitative rt-PCR assays in temperature treated Leptospira	2008
Lilium regale	Elongation factor 1-a	Different Developmental Stages & Abiotic Stress	Evaluation of putative reference genes for quantitative real-time PCR normalization in Lilium regale during development and under stress	2016
Litsea cubeba	Elongation factor 1-alpha, Eukaryotic initiation factor 4-alpha	Different Developmental Stages & Tissues	Identification of appropriate reference genes for normalizing transcript expression by quantitative real-time PCR in Litsea cubeba	2013
Lolium multiflorum	Elongation factor 1-α-like protein	Salt Stress	Identification of the valid reference genes for quantitative RT-PCR in annual ryegrass (Lolium multiflorum) under salt stress	2015
Lucilia sericata	Elongation factor 1-alpha 1	Immune Challenge	Selection and Evaluation of Tissue Specific Reference Genes in Lucilia sericata during an Immune Challenge	2015
Lycium barbarum	Elongation factor 1 alpha-like	Fruit Development	Validation of reference genes for quantitative real-time PCR during Chinese wolfberry fruit development	2013
Mus musculus	Eukaryotic Translation Elongation Factor 2	Dextran Sodium Sulfate Experimental Colitis	Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis	2016
Musca domestica	Elongation factor 1 alpha-like	Abiotic & Biotic Stress	Selection of reference genes for quantitative gene expression studies in the house fly (Musca domestica L.) using reverse transcription quantitative real-time PCR	2013
Nicotiana tabacum	Elongation factor 1α	Different Developmental Stages & Abiotic stress	Stable internal reference genes for normalization of real-time RT-PCR in tobacco (Nicotiana tabacum) during development and abiotic stress	2010
Oreochromis niloticus	EF-1a mRNA for elongation factor 1a	Different Tissues & Bacterial Infection	Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)	2013
Oreochromis niloticus	Elongation factor 1 α	Vaccination & Infection	Evaluation and Selection of Appropriate Reference Genes for Real-Time Quantitative PCR Analysis of Gene Expression in Nile Tilapia (Oreochromis niloticus) during Vaccination and Infection	2015
Paeonia suffruticosa	Elongation factor 1 alpha	Flower Development & Different Cultivars	Selection of Reference Genes for Quantitative Real-Time PCR during Flower Development in Tree Peony (Paeonia suffruticosa Andr.)	2016
Panicum virgatum	Elongation factor 1a, Eukaryotic initiation factor 4a	Different Tissues & Abiotic Stress	Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR	2014
Pecten maximus	Eukaryotic translation elongation factor 1 alpha	Different Tissues	Selection of reference genes for quantitative RT-PCR studies on the gonad of the bivalve mollusc Pecten maximus L	2012
Pennisetum glaucum	Elongation factor-1 alpha	Abiotic Stress	Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations	2016
Pennisetum glaucum	Elongation factor 1 subunit alpha, Eukaryotic initiation factor 4A	Different Tissues & Genotypes	Cloning and validation of reference genes for normalization of gene expression studies in pearl millet Pennisetum glaucum (L.) R. Br. by quantitative real-time PCR	2015
Pinus massoniana	Elongation factor 1-beta	Different Tissues & Floral Organ Development & Abiotic Stress	Selection of Reference Genes for Real-Time Quantitative PCR in Pinus massoniana Post Nematode Inoculation	2016
Plukenetia volubilis	Elongation factor 1-alpha	Different Developmental Stages & Tissues	Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR	2015
Plutella xylostella	Elongation factor 1	Different Developmental Stages & Tissues & Abiotic Stress	Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae)	2013
Posidonia oceanica	Elongation factor 1-alpha	Abiotic Stress	Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions	2012
Pyropia yezoensis	Elongation factor 1-α, Translation initiation factor 4A	Different Developmental Stages & Abiotic Stress	Selection of reference genes for gene expression normalization in Pyropia yezoensis using quantitative real-time PCR	2014
Ruditapes philippinarum	Elongation factor 1 alpha	Copper Treated	Validation of reference genes for RT-qPCR in marine bivalve ecotoxicology: Systematic review and case study using copper treated primary Ruditapes philippinarum hemocytes	2017
Salmo salar	Elongation factor 1A A, Elongation factor 1A B	Different Tissues & Smoltification Process	Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon	2005
Scophthalmus maximus	Elongation factor-1-α	Viral Infection	Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection	2013
Sebastes schlegeli	Elongation factor-1-α	Different Developmental Stages & Tissues	Selection of reference genes for reverse transcription quantitative real-time PCR normalization in black rockfish (Sebastes schlegeli)	2013
Setaria italica	Elongation factor 1-alpha	Dehydration & Salinity Treatment	Reference genes for quantitative real-time PCR analysis in the model plant foxtail millet (Setariaitalica L.) subjected to abiotic stress conditions	2013
Solanum tuberosum	Elongation factor 1-a	Elongation factor 1-a	Selection of housekeeping genes for qRT-PCR analysis in potato tubers under cold stress	2013
Solenopsis invicta	Translation elongation factor 1	Different Developmental Stages & Castes & Tissues	Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues	2013
Spodoptera litura	Elongation factor-1	Abiotic & Biotic treatments	Identification and validation of reference genes for gene expression analysis using quantitative PCR in Spodoptera litura (Lepidoptera: Noctuidae)	2013
Stipa grandis	Elongation factor 1-beta	Abiotic Stress	Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses	2017
Tuber melanosporum	Elongation factor 1-alpha	Different Developmental Stages	Validation of reference genes for quantitative real-time PCR in Perigord black truffle (Tuber melanosporum) developmental stages	2015
Vernicia fordii	Elongation factor 1-alpha	Seed Development	Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development	2012
Vigna mungo	Elongation factor 1-alpha	Biotic and Abiotic stress	Defining reference genes for qPCR normalization to study biotic and abiotic stress responses in Vigna mungo	2013
Vitis vinifera	Elongation factor 1a	Mycotic Infection	Reference gene selection and validation for the early responses to downy mildew infection in susceptible and resistant Vitis vinifera cultivars	2013
Volvox carteri	Eukaryotic translation elongation factor 1a2	Developmental Stages & Stress Treatments	Validation of reference genes for quantitative gene expression studies in Volvox carteri using real-time RT-PCR. Molecular biology reports	2013
Zea mays	Elongation factor 1 alpha	Abiotic Stresses & Hormone Treatment & Tissue Types	Validation of potential reference genes for qPCR in maize across abiotic stresses, hormone treatments, and tissue types	2014
Panax ginseng	Elongation factor 1-gamma	Different Tissues & Abiotic Stress	Validation of Suitable Reference Genes for Quantitative Gene Expression Analysis in Panax ginseng	2015
Arachis hypogaea	Elongation factor 1B	Different Tissues	Evaluation and validation of reference genes for normalization of quantitative real-time PCR based gene expression studies in peanut	2013
Arachis hypogaea	Elongation factor 1b	Different Developmental Stages & Abiotic Stress	Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR	2012
Corynebacterium pseudotuberculosis	Elongation factor P	Abiotic Stress	Reference genes for RT-qPCR studies in Corynebacterium pseudotuberculosis identified through analysis of RNA-seq data	2014
Glycine max	Eukaryotic elongation factor 1 beta	Different Tissues & Abiotic Stress	Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions	2012
Glycine max	Elongation factor 1-beta	Hypoxic Condition	Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions	2014
Panonychus citri	Elongation factor-1 alpha	Different Developmental Stages & Abiotic Stress	Evaluation of suitable reference genes for quantitative RT-PCR during development and abiotic stress in Panonychus citri (McGregor)(Acari: Tetranychidae)	2012
Raphanus sativus	Translation elongation factor 2	Different Tissues & Cultivars & Developmental Stages	Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR	2012
Sesamia inferens	Elongation factor 1	Different Tissues & Developmental Stages	Exploring valid reference genes for quantitative real-time PCR analysis in Sesamia inferens (Lepidoptera: Noctuidae)	2015

External Links

• NCBI COnserved Protein Domain Family
• WikiPedia
• Pfam