Difference between revisions of "Gentiana macrophylla"

From ICG
Jump to navigation Jump to search
 
(18 intermediate revisions by 7 users not shown)
Line 1: Line 1:
 
==Description==
 
==Description==
[[File:REFqPCR2016009-1.jpg|right|227px|]]
+
[[File:Gentiana macrophylla.png|right|200px|link=Gentiana macrophylla]]
* Gentiana macrophylla Pall. is a well-known medicinal plant in the Gentianaceae family. Its dried roots, ‘Qinjiao,have been used in traditional Chinese medicine for over 2000 years, usually as an ingredient in numerous formulae. The biological and pharmacological effects of Qinjiao include stomachic, choleretic, and antihepatotoxic activities.
+
* '''''Gentiana macrophylla Pall.''''' is a well-known medicinal plant in the Gentianaceae family. It is distributed mainly in China and Siberia, the dried roots, 'Qinjiao', have been used in traditional Chinese medicine for over 2000 years, usually as an ingredient in numerous formulae. Its roots contain some bitter compounds for treatment of diverse diseases such as jaundice, hepatitis, constipation, pains and rheumatism. The biological and pharmacological effects of 'Qinjiao' include stomachic, choleretic, and antihepatotoxic activities<ref name="ref1"/> <ref name="ref2"/>.
* Roots of Gentiana macrophylla Pall., a traditional Chinese drug with a long history of use for the treatment of jaundice, hepatitis, constipation, pains and rheumatism , have been chemically investigated by several groups.
+
* <font color=blue>'''Common Name:'''</font> '''Large leaf gentian'''
* The plant Gentiana macrophylla (Gentianaceae), commonly known as “Qin Jiao,” is distributed mainly in China and Siberia. Its roots contain some bitter compounds and which are widely used in Chinese herbal medicine for treatment of diverse diseases, such as diabetes, apoplexy, paralysis, and rheumatism.<ref name="ref1"/> <ref name="ref2"/>.
+
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=50765 <font color=blue>'''NCBI Taxonomy'''</font>]
  
==''''' Various Experimental Conditions'''''==
+
=='''''Different Tissue Types & Abiotic Stress'''''==
===Reference Genes===
+
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
Line 13: Line 13:
 
! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
+
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
Line 21: Line 21:
 
|align="center"| SAND family protein  
 
|align="center"| SAND family protein  
 
|
 
|
* Univeral reference gene
+
* Roots, stems, and leaves
 +
* AgNO3, CuSO4, arachidonic acid, ammonium citrate, salicylic acid, methyl jasmonate
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GAJR01001128 '''GAJR01001128''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GAJR01001128 '''GAJR01001128''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:TTCATGGTGATTCTCCAGC
 
* F:TTCATGGTGATTCTCCAGC
* R:TTCATGGTGATTCTCCAGC
+
* R:TTCAAGGAAGATGACAACC
 
|align="center"|  118  
 
|align="center"|  118  
 
|align="center"| 80.0
 
|align="center"| 80.0
 
|align="center"| SYBR
 
|align="center"| SYBR
 
|-
 
|-
|align="center"| EF-1α4<ref name="ref1"/>
+
|align="center"| EF-<ref name="ref1"/>
 
|align="center"| Elongation factor 1-alpha  
 
|align="center"| Elongation factor 1-alpha  
 
|
 
|
* Univeral reference gene
+
* Roots, stems, and leaves
 +
* AgNO3, CuSO4, arachidonic acid, ammonium citrate, salicylic acid, methyl jasmonate
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GAJR01027412 '''GAJR01027412''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/GAJR01027412 '''GAJR01027412''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
 
* F:GACAAGCCTCTGCGTCTC
 
* F:GACAAGCCTCTGCGTCTC
* R:GACAAGCCTCTGCGTCTC
+
* R:CCAGTTGGTGCAAAGGTG
 
|align="center"|  97  
 
|align="center"|  97  
 
|align="center"| 84.4
 
|align="center"| 84.4
Line 43: Line 45:
 
|}
 
|}
  
===Moleculer Types===
+
===Molecular Types===
 
*mRNA
 
*mRNA
 +
 
===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
Line 55: Line 58:
 
*'''Institution''': Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, China,
 
*'''Institution''': Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, China,
  
==Citation Statistics==
+
===Citation Statistics===
Cited by '''3''' (Based on Google Scholar [2017-06-01])
+
Cited by [https://scholar.google.com/scholar?cites=99430715842147598&as_sdt=2005&sciodt=0,5&hl=en'''6'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
Line 67: Line 70:
 
</ref>
 
</ref>
 
</references>
 
</references>
 
+
=='''Categories'''==
 
 
 
 
 
[[Category:Plants]]
 
[[Category:Plants]]
[[Category:mRNA]] 
+
[[Category:mRNA]][[Category:SYBR]]
[[Category:SYBR]]
+
[[Category:EF1α]] [[Category:SAND]]
 +
[[Category:Hormone Treatment]]  [[Category:Different Tissues]]  [[Category:Abiotic Stress]]  [[Category:Heavy Metals Treatments]]
 +
[[Category:geNorm]]
 +
[[Category:NormFinder]]
 +
[[Category:BestKeeper]]
 +
[[Category:Delta Ct]]

Latest revision as of 06:44, 1 September 2017

Description

Gentiana macrophylla.png
  • Gentiana macrophylla Pall. is a well-known medicinal plant in the Gentianaceae family. It is distributed mainly in China and Siberia, the dried roots, 'Qinjiao', have been used in traditional Chinese medicine for over 2000 years, usually as an ingredient in numerous formulae. Its roots contain some bitter compounds for treatment of diverse diseases such as jaundice, hepatitis, constipation, pains and rheumatism. The biological and pharmacological effects of 'Qinjiao' include stomachic, choleretic, and antihepatotoxic activities[1] [2].
  • Common Name: Large leaf gentian
  • NCBI Taxonomy

Different Tissue Types & Abiotic Stress

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
SAND1[1] SAND family protein
  • Roots, stems, and leaves
  • AgNO3, CuSO4, arachidonic acid, ammonium citrate, salicylic acid, methyl jasmonate
GAJR01001128
  • F:TTCATGGTGATTCTCCAGC
  • R:TTCAAGGAAGATGACAACC
118 80.0 SYBR
EF-1α[1] Elongation factor 1-alpha
  • Roots, stems, and leaves
  • AgNO3, CuSO4, arachidonic acid, ammonium citrate, salicylic acid, methyl jasmonate
GAJR01027412
  • F:GACAAGCCTCTGCGTCTC
  • R:CCAGTTGGTGCAAAGGTG
97 84.4 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Zhezhi Wang
  • Email: zhezhiwang@outlook.com
  • Institution: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, China,

Citation Statistics

Cited by 6 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 He Y, Yan H, Hua W, Huang Y, Wang Z (2016) Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla. Front Plant Sci 7, 945.
  2. Yu FR, Yu FH, Li RD, Wang R (2004) Inhibitory effects of the Gentiana macrophylla (Gentianaceae) extract on rheumatoid arthritis of rats. Journal of Ethnopharmacology 95, 77-81.

Categories