Difference between revisions of "Gluconacetobacter diazotrophicus"
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*'''Institute''': Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, Seropédica, RJ, Brazil | *'''Institute''': Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, Seropédica, RJ, Brazil | ||
===Citation Statistics=== | ===Citation Statistics=== | ||
| − | Cited by ''' | + | Cited by '''21''' (Based on Google Scholar [2017-08-10]) |
=='''References'''== | =='''References'''== | ||
Revision as of 09:01, 11 August 2017
Contents
Description
- Gluconacetobacter diazotrophicus strain PAL5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. Currently, G. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens [1][2].
- Common Name: NA
- NCBI Taxonomy
Various Culture Conditions
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| rho[3] | Rho termination factor |
|
NM_003134 |
|
132 | 58 | SYBR |
| 23S rRNA[3] | Ribosomal RNA subunit 23S |
|
NM_003135 |
|
150 | 58 | SYBR |
| rpoD[3] | RNA polymerase Sigma factor RpoD |
|
NM_003136 |
|
131 | 58 | SYBR |
Moleculer types
- mRNA
Evaluation Methods
Contact
- Name: Jean L. Simões-Araújo
- Email: jean@cnpab.embrapa.br
- Institute: Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, Seropédica, RJ, Brazil
Citation Statistics
Cited by 21 (Based on Google Scholar [2017-08-10])
References
- ↑ Péricles S. Galisa a,d,e, Helder A.P. da Silva b,e, Aline V.M. Macedo c, Verônica M. Reis d, Márcia S. Vidal e, José I. Baldani e, Jean L. Simões-Araújo. Identification and validation of reference genes to study the gene expression in Gluconacetobacter diazotrophicus grown in different carbon sources using RT-qPCR. J Microbiol Methods. 2012 Oct;91(1):1-7. doi:10.1016/j.mimet.2012.07.005. Epub 2012 Jul 16. PubMed PMID: 22814372.
- ↑ Stevenson DM, Weimer PJ. Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture. Appl Environ Microbiol. 2005 Aug;71(8):4672-8. PubMed PMID: 16085862; PubMed Central PMCID: PMC1183361.
- ↑ 3.0 3.1 3.2 Galisa, Péricles S., et al. "Identification and validation of reference genes to study the gene expression in Gluconacetobacter diazotrophicus grown in different carbon sources using RT-qPCR." Journal of microbiological methods 91.1 (2012): 1-7.
