Difference between revisions of "Glycine max"

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Nakayama T J, Rodrigues F A, Neumaier N, et al. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions[J]. Genet Mol Res, 2014, 13: 860-871.
 
Nakayama T J, Rodrigues F A, Neumaier N, et al. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions[J]. Genet Mol Res, 2014, 13: 860-871.
 
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Revision as of 12:15, 15 June 2017

Description

Soybean 1.png
  • Soybean is an economically important crop worldwide, which provides important source of vegetable oil and proteins. It is the world's major agricultural crop with multiple uses including as human and animal food, biofuel, feedstock, industrial products, and cosmetics.

Aluminum, Cadmium, Heat Stresses

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
TUA4[1] Tubulin alpha-4
  • Al stress in root tips
  • heat stress in roots and leaves
 LOC100781185
  • F:CATACCCTAGAATCCATTTC-3
  • R:TGTACTTTCCGTGACGAG-3
 159 60 SYBR
Fbox[1] Glycine max F-box protein SKIP16-like
  • Cd stress in roots and leaves
 LOC100809876
  • F:AGATAGGGAAATTGTGCAGGT-3
  • R:CTAATGGCAATTGCAGCTCTC-3
 93 60 SYBR
UKN2[1] Hypothetical protein
  • Cd stress in roots and leaves
  • heat stress in roots and leaves
 LOC100789577
  • F:TGTGCTCTGTGAAGAGATTG-3
  • R:TCATAATCTGTGTGCAGTTC-3
 156 60 SYBR
60S[1] 60S ribosome protein
  • Cd stress for roots and leaves
 LOC100778077
  • F:AAAGTGGACCAAGGCATATCGTCG-3
  • R:TCAGGACATTCTCCGCAAGATTCC-3
 125 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Yan Li
  • Email: yanli1@njau.edu.cn
  • Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, China

Diverse Biotic Stresses

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ABCT[2] ATP-binding cassette transporter
  • BPMV and SBA stress
 Glyma.12g020500
  • F:CTTTGCTTTTATTCCGAATGG
  • R:GCCTGCTTCAGATAAAATAGAT
 166 79.5 SYBR
FBOX[2] F-box only protein
  • BPMV and SBA stress
 Glyma.12g051100
  • F:GAAAGCAGAAAGATGGGGTTGG
  • R:CACACACGCCACTCTCGCAA
 99 80 SYBR
TUA4[2] Tubulin alpha-4
  • PMD stress
  • TSSM stress
 Glyma.20g136000
  • F:TGTGTACCAGTACCGATGTGGT
  • R:GCACTCCCGTGCCCGTAA
 150 79.5 SYBR
CYP[2] Cyclophilin
  • PMD stress
 Glyma.12g024700
  • F:GTCGAGGGGATGGACGTC
  • R:CGACACATTCAGAGCCACCGA
 158 85 SYBR
TUB4[2] Tubulin beta-4
  • TSSM stress
 Glyma.19g127700
  • F:TTCTCTGCACTCTTCATCAAGCTC
  • R:CACACCACTTCCCAGAACTTG
 117 83 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Raman Bansal
  • Email: bansal.67@osu.edu
  • Institution: Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, 44691, United States of America

Various Abiotic Stress Conditions

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Fbox[3] F-box protein family
  • root and shoot tissues of soybean seedlings with dehydration treatment
  • root and shoot tissues of soybean seedlings under salt, ABA and cold stress
 Glyma12g05510
  • F:GATCAGCAATTATGCACAACG
  • R:CTAATGGCAATTGCAGCTCTC
 118 60 SYBR
ABC[3] ATP-binding cassette transporter
  • root and shoot tissues of soybean seedlings with dehydration treatment
 Glyma12g02310
  • F:GAGCTATGAATTGCCTGATGG
  • R:CCGCCACCATTCAGATTATGT
 106 60 SYBR
60s[3] 60s Ribosomal protein L30
  • root and shoot tissues of soybean seedlings with dehydration treatment
  • root and shoot tissues of soybean seedlings under cold & ABA stress
 Glyma17g05270
  • F:AAAGTGGACCAAGGCATATCGTCG
  • R:TCAGGACATTCTCCGCAAGATTCC
 125 60 SYBR
ELF1b[3] Eukaryotic elongation factor 1 beta
  • root and shoot tissues of soybean seedlings under salt & ABA stress
 Glyma02g44460
  • F:GTTGAAAAGCCAGGGGACA
  • R:TCTTACCCCTTGAGCGTGG
 118 60 SYBR
IDE[3] Insulin-degrading enzyme
  • root and shoot tissues of soybean seedlings under salt stress
  • root and shoot tissues of soybean seedlings under cold stress
 AW310136
  • F:ATGAATGACGGTTCCCATGTA
  • R:GGCATTAAGGCAGCTCACTCT
 131 60 SYBR
Act27[3] Actin
  • root and shoot tissues of soybean seedlings under cold stress
 Glyma19g32990
  • F:CTTCCCTCAGCACCTTCCAA
  • R:GGTCCAGCTTTCACACTCCAT
 119 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Henry T. Nguyen
  • Email: nguyenhenry@missouri.edu
  • Institution: National Center for Soybean Biotechnology and Division of Plant Sciences,University of Missouri, Columbia, Missouri, United States of America

Hypoxic Conditions

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ELF1B [4] Elongation factor 1-beta
  • flooding stress
 Glyma02g44460
  • F:GTTGAAAAGCCAGGGGACA-3
  • R:TCTTACCCCTTGAGCGTGG-3
 118 60 SYBR
ACTB [4] actin-11
  • flooding stress
 Glyma15g05570
  • F:GAGCTATGAATTGCCTGATGG-3
  • R:CGTTTCATGAATTCCAGTAGC-3
 118 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: A.L. Nepomuceno
  • Email: alexandre.nepomuceno@embrapa.br
  • Institution: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brasil

References

  1. ↑ 1.0 1.1 1.2 1.3 Gao M, Liu Y, Ma X, et al. Evaluation of Reference Genes for Normalization of Gene Expression Using Quantitative RT-PCR under Aluminum, Cadmium, and Heat Stresses in Soybean[J]. PloS one, 2017, 12(1): e0168965.
  2. ↑ 2.0 2.1 2.2 2.3 2.4 Bansal R, Mittapelly P, Cassone B J, et al. Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses[J]. PloS one, 2015, 10(8): e0134890.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Le D T, Aldrich D L, Valliyodan B, et al. Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions[J]. PLoS One, 2012, 7(9): e46487.
  4. ↑ 4.0 4.1 Nakayama T J, Rodrigues F A, Neumaier N, et al. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions[J]. Genet Mol Res, 2014, 13: 860-871.
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