Description
- Soybean is an economically important crop worldwide, which provides important source of vegetable oil and proteins. It is the world's major agricultural crop with multiple uses including as human and animal food, biofuel, feedstock, industrial products, and cosmetics.
Aluminum, Cadmium, Heat Stresses
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| TUA4[1]
|
Tubulin alpha-4
|
- Al stress in root tips
- heat stress in roots and leaves
|
LOC100781185
|
- F:CATACCCTAGAATCCATTTC-3
- R:TGTACTTTCCGTGACGAG-3
|
159
|
60
|
SYBR
|
| Fbox[1]
|
Glycine max F-box protein SKIP16-like
|
- Cd stress in roots and leaves
|
LOC100809876
|
- F:AGATAGGGAAATTGTGCAGGT-3
- R:CTAATGGCAATTGCAGCTCTC-3
|
93
|
60
|
SYBR
|
| UKN2[1]
|
Hypothetical protein
|
- Cd stress in roots and leaves
- heat stress in roots and leaves
|
LOC100789577
|
- F:TGTGCTCTGTGAAGAGATTG-3
- R:TCATAATCTGTGTGCAGTTC-3
|
156
|
60
|
SYBR
|
| 60S[1]
|
60S ribosome protein
|
- Cd stress for roots and leaves
|
LOC100778077
|
- F:AAAGTGGACCAAGGCATATCGTCG-3
- R:TCAGGACATTCTCCGCAAGATTCC-3
|
125
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Yan Li
- Email: yanli1@njau.edu.cn
- Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, China
Citation Statistics
Cited by 0 (Based on Google Scholar [2017-06-16])
Diverse Biotic Stresses
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| ABCT[2]
|
ATP-binding cassette transporter
|
|
Glyma.12g020500
|
- F:CTTTGCTTTTATTCCGAATGG
- R:GCCTGCTTCAGATAAAATAGAT
|
166
|
79.5
|
SYBR
|
| FBOX[2]
|
F-box only protein
|
|
Glyma.12g051100
|
- F:GAAAGCAGAAAGATGGGGTTGG
- R:CACACACGCCACTCTCGCAA
|
99
|
80
|
SYBR
|
| TUA4[2]
|
Tubulin alpha-4
|
|
Glyma.20g136000
|
- F:TGTGTACCAGTACCGATGTGGT
- R:GCACTCCCGTGCCCGTAA
|
150
|
79.5
|
SYBR
|
| CYP[2]
|
Cyclophilin
|
|
Glyma.12g024700
|
- F:GTCGAGGGGATGGACGTC
- R:CGACACATTCAGAGCCACCGA
|
158
|
85
|
SYBR
|
| TUB4[2]
|
Tubulin beta-4
|
|
Glyma.19g127700
|
- F:TTCTCTGCACTCTTCATCAAGCTC
- R:CACACCACTTCCCAGAACTTG
|
117
|
83
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Raman Bansal
- Email: bansal.67@osu.edu
- Institution: Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, 44691, United States of America
Citation Statistics
Cited by 9 (Based on Google Scholar [2017-06-16])
Various Abiotic Stress Conditions
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| Fbox[3]
|
F-box protein family
|
- root and shoot tissues of soybean seedlings with dehydration treatment
- root and shoot tissues of soybean seedlings under salt, ABA and cold stress
|
Glyma12g05510
|
- F:GATCAGCAATTATGCACAACG
- R:CTAATGGCAATTGCAGCTCTC
|
118
|
60
|
SYBR
|
| ABC[3]
|
ATP-binding cassette transporter
|
- root and shoot tissues of soybean seedlings with dehydration treatment
|
Glyma12g02310
|
- F:GAGCTATGAATTGCCTGATGG
- R:CCGCCACCATTCAGATTATGT
|
106
|
60
|
SYBR
|
| 60s[3]
|
60s Ribosomal protein L30
|
- root and shoot tissues of soybean seedlings with dehydration treatment
- root and shoot tissues of soybean seedlings under cold & ABA stress
|
Glyma17g05270
|
- F:AAAGTGGACCAAGGCATATCGTCG
- R:TCAGGACATTCTCCGCAAGATTCC
|
125
|
60
|
SYBR
|
| ELF1b[3]
|
Eukaryotic elongation factor 1 beta
|
- root and shoot tissues of soybean seedlings under salt & ABA stress
|
Glyma02g44460
|
- F:GTTGAAAAGCCAGGGGACA
- R:TCTTACCCCTTGAGCGTGG
|
118
|
60
|
SYBR
|
| IDE[3]
|
Insulin-degrading enzyme
|
- root and shoot tissues of soybean seedlings under salt stress
- root and shoot tissues of soybean seedlings under cold stress
|
AW310136
|
- F:ATGAATGACGGTTCCCATGTA
- R:GGCATTAAGGCAGCTCACTCT
|
131
|
60
|
SYBR
|
| Act27[3]
|
Actin
|
- root and shoot tissues of soybean seedlings under cold stress
|
Glyma19g32990
|
- F:CTTCCCTCAGCACCTTCCAA
- R:GGTCCAGCTTTCACACTCCAT
|
119
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Henry T. Nguyen
- Email: nguyenhenry@missouri.edu
- Institution: National Center for Soybean Biotechnology and Division of Plant Sciences,University of Missouri, Columbia, Missouri, United States of America
Hypoxic Conditions
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| ELF1B [4]
|
Elongation factor 1-beta
|
|
Glyma02g44460
|
- F:GTTGAAAAGCCAGGGGACA-3
- R:TCTTACCCCTTGAGCGTGG-3
|
118
|
60
|
SYBR
|
| ACTB [4]
|
actin-11
|
|
Glyma15g05570
|
- F:GAGCTATGAATTGCCTGATGG-3
- R:CGTTTCATGAATTCCAGTAGC-3
|
118
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: A.L. Nepomuceno
- Email: alexandre.nepomuceno@embrapa.br
- Institution: Laboratório de Biotecnologia Vegetal, Embrapa Soja, Londrina, PR, Brasil
References
- ↑ 1.0 1.1 1.2 1.3
Gao M, Liu Y, Ma X, et al. Evaluation of Reference Genes for Normalization of Gene Expression Using Quantitative RT-PCR under Aluminum, Cadmium, and Heat Stresses in Soybean[J]. PloS one, 2017, 12(1): e0168965.
- ↑ 2.0 2.1 2.2 2.3 2.4
Bansal R, Mittapelly P, Cassone B J, et al. Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses[J]. PloS one, 2015, 10(8): e0134890.
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5
Le D T, Aldrich D L, Valliyodan B, et al. Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions[J]. PLoS One, 2012, 7(9): e46487.
- ↑ 4.0 4.1
Nakayama T J, Rodrigues F A, Neumaier N, et al. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions[J]. Genet Mol Res, 2014, 13: 860-871.
