Difference between revisions of "Gossypium hirsutum"

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===Citation Statistics===
 
===Citation Statistics===
 
Cited by '''51''' (Based on Google Scholar [2017-06-16])
 
Cited by '''51''' (Based on Google Scholar [2017-06-16])
 +
 +
=='''Different Organs & Developmental Stages'''==
 +
 +
===Reference Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 +
|-
 +
! Gene Symbol 
 +
! Gene Name
 +
! style="width=25%　font-size:9pt　"|Application Scope
 +
! Accession Number
 +
! Primer
 +
! Size [bp]
 +
! Tm [℃]
 +
! Detection
 +
|-
 +
|align="center"| GhPP2A1<ref name="ref1"/>
 +
|align="center"| catalytic subunit of protein phosphatase 2A
 +
|
 +
* distinct plants organs
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/DT545658 '''DT545658''']
 +
|nowrap style="font-size:9pt"|
 +
* F:GATCCTTGTGGAGGAGTGGA
 +
* R:GCGAAACAGTTCGACGAGAT
 +
|align="center"| 1301
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| GhUBQ14<ref name="ref1"/>
 +
|align="center"|
 +
|
 +
* distinct plants organs
 +
* flower development
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/DW505546 '''DW505546''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CAACGCTCCATCTTGTCCTT
 +
* R:TGATCGTCTTTCCCGTAAGC
 +
|align="center"| 1502
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| GhACT4<ref name="ref1"/>
 +
|align="center"| actin gene family
 +
|
 +
*flower development
 +
*floral verticils
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AY305726 '''AY305726''']
 +
|nowrap style="font-size:9pt"|
 +
* F:TTGCAGACCGTATGAGCAAG
 +
* R:ATCCTCCGATCCAGACACTG
 +
|align="center"| 1700
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| GhFBX6<ref name="ref1"/>
 +
|align="center"| F-box family protein
 +
|
 +
*floral verticils
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/DR463903 '''DR463903''']
 +
|nowrap style="font-size:9pt"|
 +
* F:TGCCTGCAGTAAATCTGTGC
 +
* R:GGGTGAAAGGGTTTCCAAAT
 +
|align="center"| 1884
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| GhMZA<ref name="ref1"/>
 +
|align="center"| clathrin adaptor complexes medium subunit family protein
 +
|
 +
*fruit development
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/DT571956 '''DT571956''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CCGTCAGACAGATTGGAGGT
 +
* R:AAAGCAACAGCCTCAACGAC
 +
|align="center"| 1853
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|-
 +
|align="center"|  GhPTB<ref name="ref1"/>
 +
|align="center"| polypyrimidine tract-binding protein homolog
 +
|
 +
*fruit development
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/DT571956 '''DT571956''']
 +
|nowrap style="font-size:9pt"|
 +
* F:GGTTACCATTGAGGGTGTGG
 +
* R:GTGCACAAAACCAAATGCAG
 +
|align="center"| 1511
 +
|align="center"| 60~61
 +
|align="center"| SYBR
 +
|}
 +
===Moleculer Type===
 +
* mRNA
 +
===Evaluation Methods===
 +
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 +
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 +
===Contact===
 +
*'''Name''': Marcio Alves-Ferreira
 +
*'''Email''': alvesfer@biologia.ufrj.br
 +
*'''Institution''': Department of Genetics, Federal University of Rio de Janeiro-UFRJ Av Prof Rodolpho Paulo Rocco, s/n - Prédio do CCS Instituto de Biologia, 2oandar - sala A2-93, 219410-970 - Rio de Janeiro, RJ - Brasil
 +
=='''References'''==
 +
<references>
 +
<ref name="ref1">
 +
Artico S, Nardeli S M, Brilhante O, et al. Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data[J]. BMC plant biology, 2010, 10(1): 49.
 +
</ref>
 +
</references>
  
 
=='''References'''==
 
=='''References'''==

Revision as of 12:20, 16 June 2017

Description

Gossypium hirsutum-1.jpg
  • Cotton (Gossypium hirsutum L.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development.
  • It is one of the most important cash crops that provide fiber to the textile industries around the world. According to the rough estimation regarding the world production of cotton, 80% comes from Brazil, China, India, Pakistan, Turkey, USA, and Uzbekistan.
  • This crop contributes a major portion to the gross national product (GNP) of many countries[1] [2] [3].

Abiotic Stress

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GhUBQ7[1] ubiquitin extension protein 7
  • Leaves & roots under salt stress and drought stress
 DQ116441
  • F:GAATGTGGCGCCGGGACCTTC
  • R:ACTCAATCCCCACCAGCCTTCTGG
 101 60 TaqMan
GhGAPDH[1] glyceraldehyde-3-phosphate dehydroge- nase C subunit
  • Leaves & roots under salt stress and drought stress
 FJ415206
  • F:TGATGCCAAGGCTGGAATTGCTT
  • R:GTGTCGGATCAAGTCGATAACACGG
 106 60 TaqMan
GhEF1A8[1] translation elongation factor 1A-8
  • Leaves & roots under salt stress and drought stress
 DQ174257
  • F:TGAGACAGACTGTTGCTGTTGGTG
  • R:ACCAAAGTTCACTTGCCACCCTTC
 109 60 TaqMan
GhTUA10[1] alpha-tubulin 10
  • Roots under salt stress
  • Leaves & roots under drought stress
 FJ594490
  • F:CACGTGAAGATCTCGCTGCCCT
  • R:ACGGTACATCCCTCAATACTCCTCC
 104 60 TaqMan
GhCYP1[1] cydophilin 1
  • Roots under drought stress
 GQ292530
  • F:CACCATGGCCTCAAATCCCAAGGT
  • R:CTCCGCAGTGCGAGGAGTGC
 109 60 TaqMan

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Baohong Zhang
  • Email: zhangb@ecu.edu
  • Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India

Citation Statistics

Cited by 31 (Based on Google Scholar [2017-06-16])

Fiber Development & Somatic Embryogenesis

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Histone3[2] histone 3 
  • fiber development and somatic embryogenesis
 AF024716
  • F:TCAAGACTGATTTGCGTTTCCA
  • R:GCGCAAAGGTTGGTGTCTTC
 100 60 SYBR
UBQ7[2] ubiquitin extension protein 
  • fiber development and somatic embryogenesis
 DQ116441
  • F:GAAGGCATTCCACCTGACCAAC
  • R:CTTGACCTTCTTCTTCTTGTGCTTG
 198 60 SYBR
Gbpolyubiquitin-1[2] fiber polyubiquitin
  • fiber development and somatic embryogenesis
 AY375335
  • F:AGCTCGGATACGATTGATAACG
  • R:GAAGACGAAGAACAAGGGGAAG
 166 60 SYBR

Moleculer Type

  • mRNA

Evaluation Methods

Contact

  • Name: ZHANG XianLong
  • Email: xlzhang@mail.hzau.edu.cn
  • Institution: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

Citation Statistics

Cited by 51 (Based on Google Scholar [2017-06-16])

Different Organs & Developmental Stages

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GhPP2A1[1] catalytic subunit of protein phosphatase 2A
  • distinct plants organs
 DT545658
  • F:GATCCTTGTGGAGGAGTGGA
  • R:GCGAAACAGTTCGACGAGAT
 1301 60~61 SYBR
GhUBQ14[1]
  • distinct plants organs
  • flower development
 DW505546
  • F:CAACGCTCCATCTTGTCCTT
  • R:TGATCGTCTTTCCCGTAAGC
 1502 60~61 SYBR
GhACT4[1] actin gene family
  • flower development
  • floral verticils
 AY305726
  • F:TTGCAGACCGTATGAGCAAG
  • R:ATCCTCCGATCCAGACACTG
 1700 60~61 SYBR
GhFBX6[1] F-box family protein
  • floral verticils
 DR463903
  • F:TGCCTGCAGTAAATCTGTGC
  • R:GGGTGAAAGGGTTTCCAAAT
 1884 60~61 SYBR
GhMZA[1] clathrin adaptor complexes medium subunit family protein
  • fruit development
 DT571956
  • F:CCGTCAGACAGATTGGAGGT
  • R:AAAGCAACAGCCTCAACGAC
 1853 60~61 SYBR
GhPTB[1] polypyrimidine tract-binding protein homolog
  • fruit development
 DT571956
  • F:GGTTACCATTGAGGGTGTGG
  • R:GTGCACAAAACCAAATGCAG
 1511 60~61 SYBR

Moleculer Type

  • mRNA

Evaluation Methods

Contact

  • Name: Marcio Alves-Ferreira
  • Email: alvesfer@biologia.ufrj.br
  • Institution: Department of Genetics, Federal University of Rio de Janeiro-UFRJ Av Prof Rodolpho Paulo Rocco, s/n - Prédio do CCS Instituto de Biologia, 2oandar - sala A2-93, 219410-970 - Rio de Janeiro, RJ - Brasil

References

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Artico S, Nardeli S M, Brilhante O, et al. Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data[J]. BMC plant biology, 2010, 10(1): 49.
  2. ↑ 2.0 2.1 2.2 2.3 Cite error: Invalid <ref> tag; no text was provided for refs named ref2
  3. ↑ Cite error: Invalid <ref> tag; no text was provided for refs named ref3

References

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References

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