Difference between revisions of "Gossypium hirsutum"

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Cited by '''31''' (Based on Google Scholar [2017-06-16])
 
Cited by '''31''' (Based on Google Scholar [2017-06-16])
  
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=='''Fiber Development & Somatic Embryogenesis'''==
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===Reference Genes===
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{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
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! Gene Symbol 
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! Gene Name
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! style="width=25%　font-size:9pt　"|Application Scope
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! Accession Number
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! Primer
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! Size [bp]
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! Tm [℃]
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! Detection
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|-
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|align="center"| Histone3<ref name="ref2"/>
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|align="center"| histone 3 
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|
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*fiber development and somatic embryogenesis
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AF024716 '''AF024716''']
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|nowrap style="font-size:9pt"|
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* F:TCAAGACTGATTTGCGTTTCCA
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* R:GCGCAAAGGTTGGTGTCTTC
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|align="center"| 100
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|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| UBQ7<ref name="ref2"/>
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|align="center"| ubiquitin extension protein 
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|
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*fiber development and somatic embryogenesis
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/DQ116441 ''DQ116441''']
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|nowrap style="font-size:9pt"|
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* F:GAAGGCATTCCACCTGACCAAC
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* R:CTTGACCTTCTTCTTCTTGTGCTTG
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|align="center"| 198
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|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| Gbpolyubiquitin-1<ref name="ref2"/>
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|align="center"| fiber polyubiquitin
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|
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*fiber development and somatic embryogenesis
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AY375335 '''AY375335''']
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|nowrap style="font-size:9pt"|
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* F:AGCTCGGATACGATTGATAACG
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* R:GAAGACGAAGAACAAGGGGAAG
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|align="center"| 166
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|align="center"| 60
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|align="center"| SYBR
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|}
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===Moleculer Type===
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* mRNA
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===Evaluation Methods===
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* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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===Contact===
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*'''Name''': ZHANG XianLong
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*'''Email''': xlzhang@mail.hzau.edu.cn
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*'''Institution''': National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  
 
=='''References'''==
 
=='''References'''==

Revision as of 09:08, 16 June 2017

Description

Gossypium hirsutum-1.jpg
  • Cotton (Gossypium hirsutum L.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development.
  • It is one of the most important cash crops that provide fiber to the textile industries around the world. According to the rough estimation regarding the world production of cotton, 80% comes from Brazil, China, India, Pakistan, Turkey, USA, and Uzbekistan.
  • This crop contributes a major portion to the gross national product (GNP) of many countries[1] [2] [3].

Abiotic Stress

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GhUBQ7[1] ubiquitin extension protein 7
  • Leaves & roots under salt stress and drought stress
 DQ116441
  • F:GAATGTGGCGCCGGGACCTTC
  • R:ACTCAATCCCCACCAGCCTTCTGG
 101 60 TaqMan
GhGAPDH[1] glyceraldehyde-3-phosphate dehydroge- nase C subunit
  • Leaves & roots under salt stress and drought stress
 FJ415206
  • F:TGATGCCAAGGCTGGAATTGCTT
  • R:GTGTCGGATCAAGTCGATAACACGG
 106 60 TaqMan
GhEF1A8[1] translation elongation factor 1A-8
  • Leaves & roots under salt stress and drought stress
 DQ174257
  • F:TGAGACAGACTGTTGCTGTTGGTG
  • R:ACCAAAGTTCACTTGCCACCCTTC
 109 60 TaqMan
GhTUA10[1] alpha-tubulin 10
  • Roots under salt stress
  • Leaves & roots under drought stress
 FJ594490
  • F:CACGTGAAGATCTCGCTGCCCT
  • R:ACGGTACATCCCTCAATACTCCTCC
 104 60 TaqMan
GhCYP1[1] cydophilin 1
  • Roots under drought stress
 GQ292530
  • F:CACCATGGCCTCAAATCCCAAGGT
  • R:CTCCGCAGTGCGAGGAGTGC
 109 60 TaqMan

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Baohong Zhang
  • Email: zhangb@ecu.edu
  • Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India

Citation Statistics

Cited by 31 (Based on Google Scholar [2017-06-16])

Fiber Development & Somatic Embryogenesis

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Histone3[2] histone 3 
  • fiber development and somatic embryogenesis
 AF024716
  • F:TCAAGACTGATTTGCGTTTCCA
  • R:GCGCAAAGGTTGGTGTCTTC
 100 60 SYBR
UBQ7[2] ubiquitin extension protein 
  • fiber development and somatic embryogenesis
 DQ116441'
  • F:GAAGGCATTCCACCTGACCAAC
  • R:CTTGACCTTCTTCTTCTTGTGCTTG
 198 60 SYBR
Gbpolyubiquitin-1[2] fiber polyubiquitin
  • fiber development and somatic embryogenesis
 AY375335
  • F:AGCTCGGATACGATTGATAACG
  • R:GAAGACGAAGAACAAGGGGAAG
 166 60 SYBR

Moleculer Type

  • mRNA

Evaluation Methods

Contact

  • Name: ZHANG XianLong
  • Email: xlzhang@mail.hzau.edu.cn
  • Institution: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Wang M, Wang Q, Zhang B. Evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (Gossypium hirsutum L.)[J]. Gene, 2013, 530(1): 44-50.
  2. ↑ 2.0 2.1 2.2 2.3 Yan R, Liang C, Meng Z, Malik W, Zhu T, Zong X, Guo S, Zhang R. Progress in genome sequencing will accelerate molecular breeding in cotton (Gossypium spp.).
  3. ↑ Biotech. 2016 Dec;6(2):217. doi: 10.1007/s13205-016-0534-3. Epub 2016 Oct 7. Review. PubMed PMID: 28330289; PubMed Central PMCID: PMC5055485.
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