Gossypium hirsutum
Revision as of 04:35, 20 June 2017 by Xysj2012 (talk | contribs) (→Fiber Development & Somatic Embryogenesis)
Contents
Description
- Cotton (Gossypium hirsutum L.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development.
- It is one of the most important cash crops that provide fiber to the textile industries around the world. According to the rough estimation regarding the world production of cotton, 80% comes from Brazil, China, India, Pakistan, Turkey, USA, and Uzbekistan.
- This crop contributes a major portion to the gross national product (GNP) of many countries[1] [2] [3].
Abiotic Stress
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| GhUBQ7[1] | ubiquitin extension protein 7 |
|
DQ116441 |
|
101 | 60 | TaqMan |
| GhGAPDH[1] | glyceraldehyde-3-phosphate dehydroge- nase C subunit |
|
FJ415206 |
|
106 | 60 | TaqMan |
| GhEF1A8[1] | translation elongation factor 1A-8 |
|
DQ174257 |
|
109 | 60 | TaqMan |
| GhTUA10[1] | alpha-tubulin 10 |
|
FJ594490 |
|
104 | 60 | TaqMan |
| GhCYP1[1] | cydophilin 1 |
|
GQ292530 |
|
109 | 60 | TaqMan |
Moleculer Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- ΔCt approach method && Related Reference
Contact
- Name: Baohong Zhang
- Email: zhangb@ecu.edu
- Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India
Citation Statistics
Cited by 31 (Based on Google Scholar [2017-06-16])
Different Developmental Stages
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| Histone3[2] | histone 3 |
|
AF024716 |
|
100 | 60 | SYBR |
| UBQ7[2] | ubiquitin extension protein |
|
DQ116441 |
|
198 | 60 | SYBR |
| Gbpolyubiquitin-1[2] | fiber polyubiquitin |
|
AY375335 |
|
166 | 60 | SYBR |
Moleculer Type
- mRNA
Evaluation Methods
Contact
- Name: ZHANG XianLong
- Email: xlzhang@mail.hzau.edu.cn
- Institution: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
Citation Statistics
Cited by 51 (Based on Google Scholar [2017-06-16])
Different Developmental Stages & Tissues
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| GhPP2A1[4] | catalytic subunit of protein phosphatase 2A |
|
DT545658 |
|
1301 | 60~61 | SYBR |
| GhUBQ14[4] | ubiquilin |
|
DW505546 |
|
1502 | 60~61 | SYBR |
| GhACT4[4] | actin gene family |
|
AY305726 |
|
1700 | 60~61 | SYBR |
| GhFBX6[4] | F-box family protein |
|
DR463903 |
|
1884 | 60~61 | SYBR |
| GhMZA[4] | clathrin adaptor complexes medium subunit family protein |
|
DT571956 |
|
1853 | 60~61 | SYBR |
| GhPTB[4] | polypyrimidine tract-binding protein homolog |
|
DT571956 |
|
1511 | 60~61 | SYBR |
Moleculer Type
- mRNA
Evaluation Methods
Contact
- Name: Marcio Alves-Ferreira
- Email: alvesfer@biologia.ufrj.br
- Institution: Department of Genetics, Federal University of Rio de Janeiro-UFRJ Av Prof Rodolpho Paulo Rocco, s/n - Prédio do CCS Instituto de Biologia, 2oandar - sala A2-93, 219410-970 - Rio de Janeiro, RJ - Brasil
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Wang M, Wang Q, Zhang B. Evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (Gossypium hirsutum L.)[J]. Gene, 2013, 530(1): 44-50.
- ↑ 2.0 2.1 2.2 2.3 Tu L L, Zhang X L, Liu D Q, et al. Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis[J]. Chinese Science Bulletin, 2007, 52(22): 3110-3117.
- ↑ Yan R, Liang C, Meng Z, Malik W, Zhu T, Zong X, Guo S, Zhang R. Progress in genome sequencing will accelerate molecular breeding in cotton (Gossypium spp.).
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Artico S, Nardeli S M, Brilhante O, et al. Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data[J]. BMC plant biology, 2010, 10(1): 49.
