Description
- Cotton (Gossypium hirsutum L.) is the single most important spinning fiber that has economic significance worldwide. Cotton is one of the most value-added crops and an excellent model system for the analysis of polyploidization and cell development.
- It is one of the most important cash crops that provide fiber to the textile industries around the world. According to the rough estimation regarding the world production of cotton, 80% comes from Brazil, China, India, Pakistan, Turkey, USA, and Uzbekistan.
- This crop contributes a major portion to the gross national product (GNP) of many countries[1] [2] [3].
Abiotic Stress
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| GhUBQ7[1]
|
ubiquitin extension protein 7
|
- Leaves & roots under salt stress and drought stress
|
DQ116441
|
- F:GAATGTGGCGCCGGGACCTTC
- R:ACTCAATCCCCACCAGCCTTCTGG
|
101
|
60
|
TaqMan
|
| GhGAPDH[1]
|
glyceraldehyde-3-phosphate dehydroge- nase C subunit
|
- Leaves & roots under salt stress and drought stress
|
FJ415206
|
- F:TGATGCCAAGGCTGGAATTGCTT
- R:GTGTCGGATCAAGTCGATAACACGG
|
106
|
60
|
TaqMan
|
| GhEF1A8[1]
|
translation elongation factor 1A-8
|
- Leaves & roots under salt stress and drought stress
|
DQ174257
|
- F:TGAGACAGACTGTTGCTGTTGGTG
- R:ACCAAAGTTCACTTGCCACCCTTC
|
109
|
60
|
TaqMan
|
| GhTUA10[1]
|
alpha-tubulin 10
|
- Roots under salt stress
- Leaves & roots under drought stress
|
FJ594490
|
- F:CACGTGAAGATCTCGCTGCCCT
- R:ACGGTACATCCCTCAATACTCCTCC
|
104
|
60
|
TaqMan
|
| GhCYP1[1]
|
cydophilin 1
|
- Roots under drought stress
|
GQ292530
|
- F:CACCATGGCCTCAAATCCCAAGGT
- R:CTCCGCAGTGCGAGGAGTGC
|
109
|
60
|
TaqMan
|
Moleculer Types
Evaluation Methods
Contact
- Name: Baohong Zhang
- Email: zhangb@ecu.edu
- Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India
Citation Statistics
Cited by 31 (Based on Google Scholar [2017-06-16])
Fiber Development & Somatic Embryogenesis
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| Histone3[2]
|
histone 3
|
- Fiber development and somatic embryogenesis
|
AF024716
|
- F:TCAAGACTGATTTGCGTTTCCA
- R:GCGCAAAGGTTGGTGTCTTC
|
100
|
60
|
SYBR
|
| UBQ7[2]
|
ubiquitin extension protein
|
- Fiber development and somatic embryogenesis
|
DQ116441
|
- F:GAAGGCATTCCACCTGACCAAC
- R:CTTGACCTTCTTCTTCTTGTGCTTG
|
198
|
60
|
SYBR
|
| Gbpolyubiquitin-1[2]
|
fiber polyubiquitin
|
- Fiber development and somatic embryogenesis
|
AY375335
|
- F:AGCTCGGATACGATTGATAACG
- R:GAAGACGAAGAACAAGGGGAAG
|
166
|
60
|
SYBR
|
Moleculer Type
Evaluation Methods
Contact
- Name: ZHANG XianLong
- Email: xlzhang@mail.hzau.edu.cn
- Institution: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
Citation Statistics
Cited by 51 (Based on Google Scholar [2017-06-16])
Different Developmental Stages & Tissues
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| GhPP2A1[4]
|
catalytic subunit of protein phosphatase 2A
|
|
DT545658
|
- F:GATCCTTGTGGAGGAGTGGA
- R:GCGAAACAGTTCGACGAGAT
|
1301
|
60~61
|
SYBR
|
| GhUBQ14[4]
|
ubiquilin
|
- Distinct plants organs
- Flower development
|
DW505546
|
- F:CAACGCTCCATCTTGTCCTT
- R:TGATCGTCTTTCCCGTAAGC
|
1502
|
60~61
|
SYBR
|
| GhACT4[4]
|
actin gene family
|
- Flower development
- Floral verticils
|
AY305726
|
- F:TTGCAGACCGTATGAGCAAG
- R:ATCCTCCGATCCAGACACTG
|
1700
|
60~61
|
SYBR
|
| GhFBX6[4]
|
F-box family protein
|
|
DR463903
|
- F:TGCCTGCAGTAAATCTGTGC
- R:GGGTGAAAGGGTTTCCAAAT
|
1884
|
60~61
|
SYBR
|
| GhMZA[4]
|
clathrin adaptor complexes medium subunit family protein
|
|
DT571956
|
- F:CCGTCAGACAGATTGGAGGT
- R:AAAGCAACAGCCTCAACGAC
|
1853
|
60~61
|
SYBR
|
| GhPTB[4]
|
polypyrimidine tract-binding protein homolog
|
|
DT571956
|
- F:GGTTACCATTGAGGGTGTGG
- R:GTGCACAAAACCAAATGCAG
|
1511
|
60~61
|
SYBR
|
Moleculer Type
Evaluation Methods
Contact
- Name: Marcio Alves-Ferreira
- Email: alvesfer@biologia.ufrj.br
- Institution: Department of Genetics, Federal University of Rio de Janeiro-UFRJ Av Prof Rodolpho Paulo Rocco, s/n - Prédio do CCS Instituto de Biologia, 2oandar - sala A2-93, 219410-970 - Rio de Janeiro, RJ - Brasil
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5
Wang M, Wang Q, Zhang B. Evaluation and selection of reliable reference genes for gene expression under abiotic stress in cotton (Gossypium hirsutum L.)[J]. Gene, 2013, 530(1): 44-50.
- ↑ 2.0 2.1 2.2 2.3
Tu L L, Zhang X L, Liu D Q, et al. Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis[J]. Chinese Science Bulletin, 2007, 52(22): 3110-3117.
- ↑
Yan R, Liang C, Meng Z, Malik W, Zhu T, Zong X, Guo S, Zhang R. Progress in genome sequencing will accelerate molecular breeding in cotton (Gossypium spp.).
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5
Artico S, Nardeli S M, Brilhante O, et al. Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data[J]. BMC plant biology, 2010, 10(1): 49.
