Difference between revisions of "Haliotis rufescens"
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==Description== | ==Description== | ||
| − | [[File: | + | [[File:Haliotis rufescens.png|right|200px|link=Haliotis rufescens]] |
| − | * | + | *'''''Haliotis rufescens''''' is one of the most important species for aquaculture in Baja California, México. The longitudinal cross-section of ''H. rufescens'' shows two types of microstructure: an outer prismatic layer, and an inner nacreous layer. The two forms of CaCO3, calcite and aragonite constitute the inorganic component of this ceramic/organic composite. Currently, there is an apparent skewed sex ratio, where males are changing to females, and this affects broodstock availability to obtain larvae at commercial scale. Increasing interest has been put in studying aspects such as reproduction, thermoregulation and endocrinology<ref name="ref1"/><ref name="ref2"/>. |
| − | + | * <font color=blue>'''Common Name:'''</font> '''Red abalone''' | |
| − | + | * [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=6454 <font color=blue>'''NCBI Taxonomy'''</font>] | |
| − | =='''''Different | + | |
| − | === | + | =='''''Different Tissues'''''== |
| + | ===Internal Control Genes=== | ||
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
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! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
| − | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
| Line 18: | Line 19: | ||
|- | |- | ||
|align="center"| HPRTI<ref name="ref1"/> | |align="center"| HPRTI<ref name="ref1"/> | ||
| − | |align="center"| | + | |align="center"| Hypoxanthine phosphoribosyltransferase 1 |
| | | | ||
* Gill and gonad/digestive gland | * Gill and gonad/digestive gland | ||
| Line 30: | Line 31: | ||
|- | |- | ||
|align="center"| RPL5<ref name="ref1"/> | |align="center"| RPL5<ref name="ref1"/> | ||
| − | |align="center"| | + | |align="center"| Ribosomal protein L5 |
| | | | ||
* Head and gonad/digestive gland | * Head and gonad/digestive gland | ||
| Line 42: | Line 43: | ||
|} | |} | ||
| − | === | + | ===Molecular Types=== |
*mRNA | *mRNA | ||
| + | |||
===Evaluation Methods=== | ===Evaluation Methods=== | ||
* [https://genorm.cmgg.be/ '''geNorm method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference'''] | * [https://genorm.cmgg.be/ '''geNorm method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference'''] | ||
| Line 54: | Line 56: | ||
===Citation Statistics=== | ===Citation Statistics=== | ||
| − | Cited by '''5''' (Based on Google Scholar [2017- | + | Cited by [https://scholar.google.com/scholar?cites=4920327290200415835&as_sdt=2005&sciodt=0,5&hl=en '''5'''] (Based on Google Scholar [2017-09-01]) |
| − | |||
=='''References'''== | =='''References'''== | ||
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</ref> | </ref> | ||
</references> | </references> | ||
| − | [[Category:Animals]] | + | =='''Categories'''== |
| + | [[Category:Animals]][[Category:mRNA]][[Category:SYBR]] | ||
| + | [[Category:HPRT]] [[Category:RPL]] | ||
| + | [[Category:Different Tissues]] | ||
| + | |||
| + | [[Category:geNorm]] | ||
| + | [[Category:NormFinder]] | ||
Latest revision as of 08:21, 1 September 2017
Contents
Description
- Haliotis rufescens is one of the most important species for aquaculture in Baja California, México. The longitudinal cross-section of H. rufescens shows two types of microstructure: an outer prismatic layer, and an inner nacreous layer. The two forms of CaCO3, calcite and aragonite constitute the inorganic component of this ceramic/organic composite. Currently, there is an apparent skewed sex ratio, where males are changing to females, and this affects broodstock availability to obtain larvae at commercial scale. Increasing interest has been put in studying aspects such as reproduction, thermoregulation and endocrinology[1][2].
- Common Name: Red abalone
- NCBI Taxonomy
Different Tissues
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| HPRTI[1] | Hypoxanthine phosphoribosyltransferase 1 |
|
KJ188156 |
|
151 | 60 | SYBR |
| RPL5[1] | Ribosomal protein L5 |
|
KJ188157 |
|
152 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Miguel A. Del Río-Portilla
- Email: mdelrio@cicese.mx
- Institution: Selection of reference genes as internal controls for gene expression in tissues of red abalone Haliotis rufescens.
Citation Statistics
Cited by 5 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 Lopez-Landavery EA, Portillo-Lopez A, Gallardo-Escarate C, Del Rio-Portilla MA (2014) Selection of reference genes as internal controls for gene expression in tissues of red abalone Haliotis rufescens (Mollusca, Vetigastropoda; Swainson, 1822). Gene 549, 258-265.
- ↑ Menig R, Meyers MH, Meyers MA, Vecchio KS (2000) Quasi-static and dynamic mechanical response of Haliotis rufescens (abalone) shells. Acta Materialia 48, 2383-2398.
