Hordeum vulgare

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Description

REFqPCR2015021-1.jpg
  • Hordeum vulgare is one of the most important cereal crops cultivated in the world. Its production in southern Australia is particularly constrained by cyclic and plant terminal drought in addition to a number of biotic, abiotic and physiochemical subsoil stresses. As a model plant with a large amount of genetic and genomic data available, it is still challenging to develop varieties with improved and stable yield in strssed environments with ongoing climate change. Substantial changes are needed for crop improvement approaches[1] [2].
  • Common Name: Barley
  • NCBI Taxonomy

Abiotic & Biotic Stress

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
Hvu-ADP[1] ADP-ribosylation factor 1-like protein
  • Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
NA
  • F:GCTCTCCAACAACATTGCCAAC
  • R:GAGACATCCAGCATCATTCATTCC
77 60 SYBR
Hvu-snoR14[1] Hvu-snoR14
  • Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
NA
  • F:GATGTTTATGTATGATAGTCTGTC
  • R:GTCGGGATGTATGCGTGTC
67 60 SYBR
Hvu-snoR23[1] Hvu-snoR23
  • Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
NA
  • F:TCGGCAGTGGTGTCATC
  • R:CTCAGTGGAAAGAGAAGTCG
64 60 SYBR

Molecular Types

  • mRNA & miRNA

Evaluation Methods

Contact

  • Name: S.K. Bhure
  • Email: sdbhure@rediffmail.com
  • Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India

Citation Statistics

Cited by 16 (Based on Google Scholar [2017-06-16])

Different Developmental stages

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ADP[2] ADP-ribozylation factor 1
  • Drought-induced changes at the seedling stage
  • Comparison between seedling and heading stage
NA
  • F:CGTGACGCTGTGTTGCTTGT
  • R: CCGCATTCATCGCATTAGG
61 60 SYBR
UBI[2] ubiquitin
  • Drought-induced changes at the seedling stage
NA
  • F:TCGCCGTCCTCCAGTTCTAC
  • R:CCTTCCTGAGCCTGGTTACCT
63 59 SYBR
ACT[2] Actin
  • During heading
NA
  • F:CGACAATGGAACCGGAATG
  • R: CCCTTGGCGCATCATCTC
56 58 SYBR
GAPDH[2] glyceraldehyde-3-phosphate dehydrogenase
  • During heading
NA
  • F:TTCGGCGAGAAGCCAGTTA
  • R:CCTCACCCCACGGGATCT
64 58 SYBR
HSP90[2] Heat shock protein 90
  • Comparison between seedling and heading stage
NA
  • F: AAGATGGAGGAGGTCGACTAAGC
  • R:GCAGGGCATGGACATACGA
59 59 SYBR

Moleculer types

  • mRNA

Evaluation Methods

Contact

  • Name: Marcin Rapacz
  • Email: rrrapacz@cyf-kr.edu.pl
  • Institution: Department of Plant Physiology, University of Agriculture in Krakow, Podłu_ zna 3, 30-239 Krako´w, Poland

Citation Statistics

Cited by 23 (Based on Google Scholar [2017-06-16])

References

  1. 1.0 1.1 1.2 1.3 Ferdous J, Li Y, Reid N, et al. (2015) Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions. PLoS One 10, e0118503
  2. 2.0 2.1 2.2 2.3 2.4 2.5 Rapacz M, Stępień A, Skorupa K. Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (Hordeum vulgare L.): the effects of developmental stage and leaf age[J]. Acta physiologiae plantarum, 2012, 34(5): 1723-1733.