Description
- Barley (Hordeum vulgare) is one of the most important cereal crops cultivated in the world and, with a large amount of genetic and genomic data available, including its full genome sequence, is a model for cereal genomics studies.
- The average yield of Australian barley is 2 t/ha [1], which is below the world average of 3 t/ha. Barley production in southern Australia is particularly constrained by cyclic and terminal drought in addition to a number of biotic, abiotic and physiochemical subsoil stresses.
- Yield is a complex quantitative trait whose expression is highly influenced by the environment and agronomic management. This makes phenotype-based selection slow and unreliable, especially under environments where multiple abiotic stresses prevail. Developing barley varieties with improved and stable yield in such environments is expected to be more challenging with ongoing climate change, thus requiring substantial changes in agronomic practices and crop improvement approaches. [1] [2].
Various Stress Conditions
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| Hvu-ADP[1]
|
ADP-ribosylation factor 1-like protein
|
- Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
|
NA
|
- F:GCTCTCCAACAACATTGCCAAC
- R:GCTCTCCAACAACATTGCCAAC
|
77
|
60
|
SYBR
|
| Hvu-snoR14[1]
|
Hvu-snoR14
|
- Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
|
NA
|
- F:GATGTTTATGTATGATAGTCTGTC
- R:GATGTTTATGTATGATAGTCTGTC
|
67
|
60
|
SYBR
|
| Hvu-snoR23[1]
|
Hvu-snoR23
|
- Drought, fungal infection, boron toxicity, nutrient deficiency and salinity
|
NA
|
- F:TCGGCAGTGGTGTCATC
- R:TCGGCAGTGGTGTCATC
|
64
|
60
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: S.K. Bhure
- Email: sdbhure@rediffmail.com
- Institution: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, U.P., India
Citation Statistics
Cited by 16 (Based on Google Scholar [2017-06-16])
'Different Developmental stages & Leaf Age'
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| ADP[2]
|
ADP-ribozylation factor 1
|
- drought-induced changes at the seedling stage
- comparison between these two stages
|
NA
|
- F:CGTGACGCTGTGTTGCTTGT
- R: CCGCATTCATCGCATTAGG
|
61
|
60
|
SYBR
|
| UBI[2]
|
ubiquitin
|
- drought-induced changes at the seedling stage
|
NA
|
- F:TCGCCGTCCTCCAGTTCTAC
- R:CCTTCCTGAGCCTGGTTACCT
|
63
|
59
|
SYBR
|
| ACT[2]
|
Actin
|
|
NA
|
- F:CGACAATGGAACCGGAATG
- R: CCCTTGGCGCATCATCTC
|
56
|
58
|
SYBR
|
| GAPDH[2]
|
glyceraldehyde-3-phosphate dehydrogenase
|
|
NA
|
- F:TTCGGCGAGAAGCCAGTTA
- R:CCTCACCCCACGGGATCT
|
64
|
58
|
SYBR
|
| HSP90[2]
|
Heat shock protein 90
|
- comparison between these two stages
|
NA
|
- F: AAGATGGAGGAGGTCGACTAAGC
- R:GCAGGGCATGGACATACGA
|
59
|
59
|
SYBR
|
Moleculer types
Evaluation Methods
Contact
- Name: Marcin Rapacz
- Email: rrrapacz@cyf-kr.edu.pl
- Institute: Department of Plant Physiology, University of Agriculture in Krakow, Podłu_ zna 3, 30-239 Krako´w, Poland
References
- ↑ 1.0 1.1 1.2 1.3
Ferdous J, Li Y, Reid N, et al. (2015) Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions. PLoS One 10, e0118503
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5
Rapacz M, Stępień A, Skorupa K. Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (Hordeum vulgare L.): the effects of developmental stage and leaf age[J]. Acta physiologiae plantarum, 2012, 34(5): 1723-1733.
