Difference between revisions of "Lactuca sativa"

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===Citation Statistics===
 
===Citation Statistics===
 
Cited by '''8''' (Based on Google Scholar [2017-08-10])
 
Cited by '''8''' (Based on Google Scholar [2017-08-10])
 +
 +
=='''''Abiotic stresses'''''==
 +
===Internal Control Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 +
|-
 +
! Gene Symbol 
 +
! Gene Name
 +
! style="width=25% font-size:9pt "|Application Scope
 +
! Accession Number
 +
! Primers (5'-3')<br>[Forward/Reverse]
 +
! Size [bp]
 +
! Tm [℃]
 +
! Detection
 +
|-
 +
|align="center"| MIR169<ref name="ref1"/>
 +
|align="center"| PROTEIN PHOSPHATASE 2A-1
 +
|
 +
* Leaves
 +
* Drought, salt, heavy metal, and UV-c irradiation conditions
 +
* Application of abscisic acid (ABA)
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:ATTCATGGTCAATTCTACGATCTGGT
 +
* R:GAATAATACCCGCGATCAACATAATC
 +
|align="center"|  107
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| MIR171/170<ref name="ref1"/>
 +
|align="center"| TAP42-INTERACTING PROTEIN OF 41 kDa
 +
|
 +
* Leaves
 +
* Drought, salt, heavy metal, and UV-c irradiation conditions
 +
* Application of abscisic acid (ABA)
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:TTTGTATGGAGATGAATTGGCTGATA
 +
* R:CGTAAGAGAAGAAACCAACAGCTAGG
 +
|align="center"|  90
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| MIR172<ref name="ref1"/>
 +
|align="center"| TAP42-INTERACTING PROTEIN OF 41 kDa
 +
|
 +
* Leaves
 +
* Drought, salt, heavy metal, and UV-c irradiation conditions
 +
* Application of abscisic acid (ABA)
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:TTTGTATGGAGATGAATTGGCTGATA
 +
* R:CGTAAGAGAAGAAACCAACAGCTAGG
 +
|align="center"|  90
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| PAL<ref name="ref1"/>
 +
|align="center"| TAP42-INTERACTING PROTEIN OF 41 kDa
 +
|
 +
* Leaves
 +
* Drought, salt, heavy metal, and UV-c irradiation conditions
 +
* Application of abscisic acid (ABA)
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:TTTGTATGGAGATGAATTGGCTGATA
 +
* R:CGTAAGAGAAGAAACCAACAGCTAGG
 +
|align="center"|  90
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| HPPD<ref name="ref1"/>
 +
|align="center"| TAP42-INTERACTING PROTEIN OF 41 kDa
 +
|
 +
* Leaves
 +
* Drought, salt, heavy metal, and UV-c irradiation conditions
 +
* Application of abscisic acid (ABA)
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:TTTGTATGGAGATGAATTGGCTGATA
 +
* R:CGTAAGAGAAGAAACCAACAGCTAGG
 +
|align="center"|  90
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|}
 +
 +
===Molecular Types===
 +
*mRNA
 +
===Evaluation Methods===
 +
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 +
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 +
 +
===Contact===
 +
*'''Name''': Cesar Valmor Rombaldi
 +
*'''Email''': cesarvrf@ufpel.edu.br
 +
*'''Institution''':  Faculdade de agronomia eliseu Maciel, campus Universitário s/n, Universidade Federal de Pelotas, caixa Postal 354, Pelotas, rS ceP 96010-900, Brazil
 +
 +
===Citation Statistics===
 +
Cited by '''33''' (Based on Google Scholar [2017-08-10])
  
 
=='''References'''==
 
=='''References'''==

Revision as of 07:49, 14 August 2017

Description

Lactuca sativa.png
  • Lactuca sativa is a leafy vegetable cultivated throughout the course of the year. It has served as model in studies of allelopathic effects owing to its rapid germination, uniformity and sensitivity. It is harvested when plants reach maturity but before they bolt and flower. In order to avoid harvest loss and wastage it is therefore important to be able to predict and anticipate when the crop will start bolting. Understanding changes in gene expression profiles, in particular those of flowering time genes, throughout development will help to develop predictable cropping.[1] [2].
  • Common Name: Lettuce
  • NCBI Taxonomy

Different Developmental Stages

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
LsPP2A-1[1] PROTEIN PHOSPHATASE 2A-1
  • Diurnal and developmental timecourse experiments
NA
  • F:ATTCATGGTCAATTCTACGATCTGGT
  • R:GAATAATACCCGCGATCAACATAATC
107 60 SYBR
LsPP2AA3[1] PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT A3
  • Diurnal and developmental timecourse experiments
NA
  • F:CATGCAATGGTTACAAGACAAGGTAT
  • R:CAAACTCCTCCGCAAGTCTCTTC
80 60 SYBR
LsTIP41[1] TAP42-INTERACTING PROTEIN OF 41 kDa
  • Diurnal and developmental timecourse experiments
NA
  • F:TTTGTATGGAGATGAATTGGCTGATA
  • R:CGTAAGAGAAGAAACCAACAGCTAGG
90 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Stephen Jackson
  • Email: stephen.jackson@warwick.ac.uk
  • Institution: Biology Department, Federal University of Lavras (UFLA), 37.200-000, Lavras, MG, Brazil.

Citation Statistics

Cited by 8 (Based on Google Scholar [2017-08-10])

Abiotic stresses

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
MIR169[1] PROTEIN PHOSPHATASE 2A-1
  • Leaves
  • Drought, salt, heavy metal, and UV-c irradiation conditions
  • Application of abscisic acid (ABA)
NA
  • F:ATTCATGGTCAATTCTACGATCTGGT
  • R:GAATAATACCCGCGATCAACATAATC
107 60 SYBR
MIR171/170[1] TAP42-INTERACTING PROTEIN OF 41 kDa
  • Leaves
  • Drought, salt, heavy metal, and UV-c irradiation conditions
  • Application of abscisic acid (ABA)
NA
  • F:TTTGTATGGAGATGAATTGGCTGATA
  • R:CGTAAGAGAAGAAACCAACAGCTAGG
90 60 SYBR
MIR172[1] TAP42-INTERACTING PROTEIN OF 41 kDa
  • Leaves
  • Drought, salt, heavy metal, and UV-c irradiation conditions
  • Application of abscisic acid (ABA)
NA
  • F:TTTGTATGGAGATGAATTGGCTGATA
  • R:CGTAAGAGAAGAAACCAACAGCTAGG
90 60 SYBR
PAL[1] TAP42-INTERACTING PROTEIN OF 41 kDa
  • Leaves
  • Drought, salt, heavy metal, and UV-c irradiation conditions
  • Application of abscisic acid (ABA)
NA
  • F:TTTGTATGGAGATGAATTGGCTGATA
  • R:CGTAAGAGAAGAAACCAACAGCTAGG
90 60 SYBR
HPPD[1] TAP42-INTERACTING PROTEIN OF 41 kDa
  • Leaves
  • Drought, salt, heavy metal, and UV-c irradiation conditions
  • Application of abscisic acid (ABA)
NA
  • F:TTTGTATGGAGATGAATTGGCTGATA
  • R:CGTAAGAGAAGAAACCAACAGCTAGG
90 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Cesar Valmor Rombaldi
  • Email: cesarvrf@ufpel.edu.br
  • Institution: Faculdade de agronomia eliseu Maciel, campus Universitário s/n, Universidade Federal de Pelotas, caixa Postal 354, Pelotas, rS ceP 96010-900, Brazil

Citation Statistics

Cited by 33 (Based on Google Scholar [2017-08-10])

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Sgamma T, Pape J, Massiah A, Jackson S (2016) Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce. Plant Methods 12, 21.
  2. Silveira GL, Lima MGF, Reis GBD, Palmieri MJ, Andrade-Vieria LF (2017) Toxic effects of environmental pollutants: Comparative investigation using Allium cepa L. and Lactuca sativa L. Chemosphere 178, 359-367.

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